FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Ada3, Ada2b
FlyBase ID
FBig0000097851
Interaction Network
Interactions Browser links
Ada3 network
Ada2b network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0155965-1.Y2H
Reference
Muratoglu et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ada2b 

dAda2b

bait

fused to LexA DNA-binding domain

Ada3 

dAda3

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

dik (Ada3) interacts with Ada2b, but not with Ada2a.

(Muratoglu et al., 2003)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Muratoglu et al., 2003)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Muratoglu et al., 2003)
FBrf0159212-17.coIP.WB
Reference
Kusch et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Ada3 

dAda3

bait
Ada2b 

dAda2b

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kusch et al., 2003)
FBrf0159212-2.coIP.WB
Reference
Kusch et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Ada2b 

dAda2b

bait
Ada3 

dAda3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kusch et al., 2003)
FBrf0193695-2.Y2H
Reference
Ciurciu et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ada2b 

dAda2b

bait

fused to lexA DNA-binding domain

Ada3 

dAda3

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast lexA-BD/GAL4-AD

(Ciurciu et al., 2006)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Ciurciu et al., 2006)
FBrf0209543-110.coIP.WB
Reference
Weake et al., 2009
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ada3 

Ada3

bait
Ada2b 

Ada2b

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclei of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Weake et al., 2009)

The dik-Ada2b interaction is not affected in Saf6 mutant embryos.

(Weake et al., 2009)
FBrf0209543-2.coIP.MS
Reference
Weake et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Ada2b 

Ada2B

bait

FLAG tag, HA tag

Ada3 

Ada3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Weake et al., 2009)

The Drosophila SAGA complex is composed of the following subunits: Gcn5, Ada2b, dik, Sgf29, CG6506, Ada1-2, Spt3, Spt20, Nipped-A, e(y)1 (Taf9), Taf10b, Taf12, wda, Saf6, not, Sgf11, e(y)2 and Atxn7.

(Weake et al., 2009)

Tandem affinity purification of bait and identification of prey by MudPIT.

(Weake et al., 2009)
FBrf0214372-19.coIP.MS
Reference
Weake et al., 2011
Description
physical association
Assay
Collection
Source/Stage
embryonic/larval nervous system / embryonic stage 10embryonic stage 17late extended germ band stageembryonic stage 13embryonic stage 11embryonic stage 12embryonic stage 14embryonic stage 15dorsal closure stageembryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ada2b 

Ada2b

bait

HA tag, FLAG tag

Ada3 

Ada3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct in nervous system (elav-GAL4); prey produced from endogenous gene.

(Weake et al., 2011)

All SAGA subunits were present in purifications from both muscle and neuronal cells.

(Weake et al., 2011)

Proteins that non-specifically bind FLAG beads were identified in control purifications from untagged extract (OregonR) and excluded from further analysis.

(Weake et al., 2011)
FBrf0214372-2.coIP.MS
Reference
Weake et al., 2011
Description
physical association
Assay
Collection
Source/Stage
embryonic/larval muscle system / embryonic stage 10embryonic stage 17late extended germ band stageembryonic stage 13embryonic stage 11embryonic stage 12embryonic stage 14embryonic stage 15dorsal closure stageembryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ada2b 

Ada2b

bait

FLAG tag

Ada3 

Ada3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct in muscle (Mef2-GAL4); prey produced from endogenous gene.

(Weake et al., 2011)

All SAGA subunits were present in purifications from both muscle and neuronal cells.

(Weake et al., 2011)

Proteins that non-specifically bind FLAG beads were identified in control purifications from untagged extract (OregonR) and excluded from further analysis.

(Weake et al., 2011)
FBrf0221014-2.coIP.WB
Reference
Pankotai et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Ada2b 

dADA2b

bait

FLAG tag

Ada3 

dADA3

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Long and short isoforms of Ada2b can associate with dik.

(Pankotai et al., 2013)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Pankotai et al., 2013)
FBrf0241207-15.coIP.MP
Reference
Torres-Zelada et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Ada2b 

Ada2b

bait

FLAG, HA tag

Ada3 

Ada3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Torres-Zelada et al., 2019)
FBrf0241207-8.Y2H
Reference
Torres-Zelada et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ada2b 

Ada2b

bait

fused to GAL4 DNA-binding domain

Ada3 

Ada3

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Torres-Zelada et al., 2019)

Two-hybrid system: yeast GAL4-BD/GAL4-AD. The Ada2b-PA isoform was used.

(Torres-Zelada et al., 2019)
FBrf0242086-5.CH.MP
Reference
Soffers et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Ada2b 

Ada2b

bait

HA,FLAG tag

Ada3 

Ada3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Soffers et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (8)