FB2024_03 , released June 25, 2024
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Nipped-A, Ada2b
FlyBase ID
FBig0000097847
Interaction Network
Interactions Browser links
Nipped-A network
Ada2b network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0159212-10.coIP.WB
Reference
Kusch et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Ada2b 

dAda2b

bait
Nipped-A 

dTra1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Nipped-A coimmunoprecipitates with Ada2b, but not with Ada2a.

(Kusch et al., 2003)

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kusch et al., 2003)
FBrf0209543-8.coIP.MS
Reference
Weake et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Ada2b 

Ada2B

bait

FLAG tag, HA tag

Nipped-A 

Tra1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Weake et al., 2009)

The Drosophila SAGA complex is composed of the following subunits: Gcn5, Ada2b, dik, Sgf29, CG6506, Ada1-2, Spt3, Spt20, Nipped-A, e(y)1 (Taf9), Taf10b, Taf12, wda, Saf6, not, Sgf11, e(y)2 and Atxn7.

(Weake et al., 2009)

Tandem affinity purification of bait and identification of prey by MudPIT.

(Weake et al., 2009)
FBrf0214372-24.coIP.MS
Reference
Weake et al., 2011
Description
physical association
Assay
Collection
Source/Stage
embryonic/larval nervous system / embryonic stage 10embryonic stage 17late extended germ band stageembryonic stage 13embryonic stage 11embryonic stage 12embryonic stage 14embryonic stage 15dorsal closure stageembryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ada2b 

Ada2b

bait

HA tag, FLAG tag

Nipped-A 

Tra1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct in nervous system (elav-GAL4); prey produced from endogenous gene.

(Weake et al., 2011)

All SAGA subunits were present in purifications from both muscle and neuronal cells.

(Weake et al., 2011)

Proteins that non-specifically bind FLAG beads were identified in control purifications from untagged extract (OregonR) and excluded from further analysis.

(Weake et al., 2011)
FBrf0214372-7.coIP.MS
Reference
Weake et al., 2011
Description
physical association
Assay
Collection
Source/Stage
embryonic/larval muscle system / embryonic stage 10embryonic stage 17late extended germ band stageembryonic stage 13embryonic stage 11embryonic stage 12embryonic stage 14embryonic stage 15dorsal closure stageembryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ada2b 

Ada2b

bait

FLAG tag

Nipped-A 

Tra1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct in muscle (Mef2-GAL4); prey produced from endogenous gene.

(Weake et al., 2011)

All SAGA subunits were present in purifications from both muscle and neuronal cells.

(Weake et al., 2011)

Proteins that non-specifically bind FLAG beads were identified in control purifications from untagged extract (OregonR) and excluded from further analysis.

(Weake et al., 2011)
FBrf0240107-12.coIP.WB
Reference
Popova et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Nipped-A 

TRRAP

bait
Ada2b 

Ada2b

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Popova et al., 2018)
FBrf0240107-14.coIP.WB
Reference
Popova et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Ada2b 

Ada2b

bait
Nipped-A 

TRRAP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Popova et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)