FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Clk, per
FlyBase ID
FBig0000097795
Interaction Network
Interactions Browser links
Clk network
per network
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0110163-5.coIP.A
Reference
Lee et al., 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Clk 

dCLOCK

bait

HA tag, [35]S label

per 

PER

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro translation; prey produced and labeled by in vitro translation.

(Lee et al., 1999)
FBrf0200748-3.coIP.WB
Reference
Kim et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-Invitrogen
Participants
Corresponds to
Reported as
Role
Note
per 

dPER

bait

HA tag

Clk 

dClk

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kim et al., 2007)
FBrf0200748-5.coIP.WB
Reference
Kim et al., 2007
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
per 

dPER

bait
Clk 

dCLK

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Kim et al., 2007)
FBrf0210011-1.coIP.WB
Reference
Menet et al., 2010
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Clk 

CLK

bait

V5 tag

per 

PER

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Menet et al., 2010)
FBrf0210011-4.coIP.WB
Reference
Menet et al., 2010
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
per 

PER

bait
Clk 

CLK

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Menet et al., 2010)
FBrf0212171-2.coIP.WB
Reference
Sun et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
per 

per

bait

V5 tag

per 
Clk 

Clk

prey

V5 or HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
per 
conserved region 4
necessary binding region
aa 926-977

region only necessary in the absence of tim

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sun et al., 2010)
FBrf0212171-4.coIP.WB
Reference
Sun et al., 2010
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Clk 

Clk

bait
per 

per

prey

HA tag

per 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
per 
conserved region 4
necessary binding region
aa 926-977

region necessary only in light conditions, binding unaffected in dark

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Sun et al., 2010)
FBrf0212171-5.coIP.WB
Reference
Sun et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Clk 

Clk

bait

HA tag

per 

per

prey

V5 tag

per 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
per 
conserved region 4
necessary binding region
aa 926-977

region only necessary in the absence of tim

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sun et al., 2010)
FBrf0220792-3.coIP.WB
Reference
Kaasik et al., 2013
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Clk 

dClk

bait
per 

dPer

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Kaasik et al., 2013)
FBrf0225935-2.coIP.WB
Reference
Lee et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Clk 

Clk

bait

HA tag

per 

per

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lee et al., 2014)
FBrf0233199-2.coIP.WB
Reference
Fu et al., 2016
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Clk 

dCLK

bait
per 

dPER

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Positive control.

(Fu et al., 2016)

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Fu et al., 2016)
FBrf0233224-1.coIP.WB
Reference
Lee et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Clk 

dCLK

bait

HA tag

Clk 
per 

PER

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Clk 
PER binding region
necessary binding region
aa 657-707

coordinates relative to Clk-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lee et al., 2016)

The per binding region of Clk that was found to be necessary for coimmunoprecipitation (aa 657-707) was not sufficient in pull down assays. Another region of Clk (aa 380-528) was sufficient for binding in pull down assays but was not necessary in coimmunoprecipitation assays.

(Lee et al., 2016)
FBrf0233224-3.coIP.WB
Reference
Lee et al., 2016
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Clk 

dCLK

bait
Clk 
per 

PER

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Clk 
PER binding region
necessary binding region
aa 657-707

coordinates relative to Clk-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

(Lee et al., 2016)
FBrf0233224-5.PD.A
Reference
Lee et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Clk 

dCLK

bait

GST tag

Clk 
per 

PER

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Clk 
central region
sufficient binding region
aa 380-528

coordinates relative to Clk-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Lee et al., 2016)

The per binding region of Clk that was found to be necessary for coimmunoprecipitation (aa 657-707) was not sufficient in pull down assays. Another region of Clk (aa 380-528) was sufficient for binding in pull down assays but was not necessary in coimmunoprecipitation assays.

(Lee et al., 2016)
FBrf0236441-2.coIP.WB
Reference
Agrawal et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Clk 

CLK

bait

V5 tag

per 

PER

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was bodies of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Agrawal et al., 2017)
FBrf0241434-1.coIP.WB
Reference
Li et al., 2019
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Clk 

CLK

bait
per 

PER

prey

HA tag

per 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
per 
interaction domain residue 942
mutation increasing interaction
aa 942

S942A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Li et al., 2019)
FBrf0241434-2.coIP.WB
Reference
Li et al., 2019
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
per 

PER

bait

HA tag

per 
Clk 

CLK

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
per 
interaction domain residue 942
mutation increasing interaction
aa 942

S942A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Coexpression of sxc reduces the Clk-per interaction.

(Li et al., 2019)

Source was heads of transgenic fly line; bait produced from tagged transgenic construct or endogenous gene; prey produced from endogenous gene.

(Li et al., 2019)
FBrf0241434-3.coIP.WB
Reference
Li et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Clk 

CLK

bait

V5 tag

per 

PER

prey

Myc tag

per 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
per 
interaction domain residue 942
mutation increasing interaction
aa 942

S942A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Coexpression of sxc reduces the Clk-per interaction.

(Li et al., 2019)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Li et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (10)