Source was larval nuclear extract of wild-type males; bait produced from endogenous gene; prey produced from endogenous gene.
Source was larval nuclear extract of wild-type males; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction in vitro; bait produced and labeled by in vitro translation; prey produced and labeled by in vitro translation.
Interaction in vitro; bait produced and labeled by in vitro translation; prey produced and labeled by in vitro translation.
Source was cell extract of SL2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
M14K
L28P
L32P
L35P
L39R
S40P
C41R
C44G
P51L
S53P
C67Y
C107R
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was cell extract of SL2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Positive control.
Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Positive control.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic construct.
M113A,V114A,M115A,L116A, coordinates relative to msl-1-PA
aa D132A,E134A, coordinates relative to msl-1-PA.
aa I128A,L135A, coordinates relative to msl-1-PA
Interaction in vitro; bait derived from transgenic fly extract; prey produced and labeled by in vitro translation.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and insect cell system; prey produced in baculovirus and insect cell system.
Source was cell extract of SL2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from endogenous gene.
V46E, M75E, M77E triple mutation
Source was cell extract of Kc cell line; bait produced from transfected construct; prey produced from endogenous gene.
Positive control.
Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Positive control.
Source was cell extract of SL2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Factors were detected on polytene chromosomes using antibodies (to proteins) or biotinylated antisense probes (to ncRNA). The PLA signal develops only if probes to the two factors are in close proximity to each other (within tens of nanometers).
Source was larval salivary gland of wild-type fly line; proteins produced from endogenous gene.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD. Positive control.
Interaction in vitro; proteins co-produced and purified as recombinant fusion proteins in a baculovirus and Sf21 cell system.
Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell nuclear extract.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from Kc167 cell nuclear extract.
