Source was embryos of transgenic fly line; bait produced from endogenous gene; prey produced from tagged endogenous gene.

cnn and asl interact as part of a Sas-4-containing complex.

Sas-4-containing complexes were affinity-purified, then subjected to immunoprecipitation by antibodies to cnn protein.

Source was embryos of transgenic fly line; bait produced from endogenous gene; prey produced from tagged endogenous gene.

asl and cnn interact as part of a Sas-4-containing complex.

Sas-4-containing complexes were affinity-purified, then subjected to immunoprecipitation by antibodies to asl protein.

Source was embryos of transgenic fly line; proteins produced from endogenous gene.

Characterization of Sas-4 complexes shows that cnn and asl cosediment in a narrow range of fractions at 7S.

TAP-tagged Sas-4 was immunoprecipitated from "high-speed lysates" depleted of mature centrosomes, and purified complexes were subjected to velocity sedimentation.

Source was embryos of wild-type fly line; proteins produced from endogenous gene.

cnn and asl co-fractionate as part of a Sas-4-containing S-CAP complex (centrosomal scaffold).

Sas-4 complexes were immunoprecipitated from "high-speed lysates" depleted of mature centrosomes and different Sas-4-containing complexes were fractionated on a sucrose gradient.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).