Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Tandem affinity purification of bait and identification of prey by MudPIT.
Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct in muscle (Mef2-GAL4); prey produced from endogenous gene.
All SAGA subunits were present in purifications from both muscle and neuronal cells.
Proteins that non-specifically bind FLAG beads were identified in control purifications from untagged extract (OregonR) and excluded from further analysis.
Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct in nervous system (elav-GAL4); prey produced from endogenous gene.
All SAGA subunits were present in purifications from both muscle and neuronal cells.
Proteins that non-specifically bind FLAG beads were identified in control purifications from untagged extract (OregonR) and excluded from further analysis.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.