FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Plp, cnn
FlyBase ID
FBig0000097183
Interaction Network
Interactions Browser links
Plp network
cnn network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0212603-11.coIP.WB
Reference
Conduit et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Plp 

Plp

bait
cnn 

cnn

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Conduit et al., 2010)
FBrf0212603-3.coIP.WB
Reference
Conduit et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cnn 

cnn

bait
Plp 

Plp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Conduit et al., 2010)
FBrf0216370-16.coIP.WB
Reference
Gopalakrishnan et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 12blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cnn 

CNN

bait
Plp 

D-PLP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from endogenous gene; prey produced from tagged endogenous gene.

(Gopalakrishnan et al., 2011)

cnn and cp309 interact as part of a Sas-4-containing complex.

(Gopalakrishnan et al., 2011)

Sas-4-containing complexes were affinity-purified, then subjected to immunoprecipitation by antibodies to cnn protein.

(Gopalakrishnan et al., 2011)
FBrf0216370-25.CS.WB
Reference
Gopalakrishnan et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 12blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cnn 

CNN

neutral component
cnn 

CNN

neutral component
cnn 

CNN

neutral component
Plp 

D-PLP

neutral component
Plp 

D-PLP

neutral component
Plp 

D-PLP

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; proteins produced from endogenous gene.

(Gopalakrishnan et al., 2011)

Characterization of Sas-4 complexes shows that cnn and cp309 cosediment in a narrow range of fractions at 7S.

(Gopalakrishnan et al., 2011)

TAP-tagged Sas-4 was immunoprecipitated from "high-speed lysates" depleted of mature centrosomes, and purified complexes were subjected to velocity sedimentation.

(Gopalakrishnan et al., 2011)
FBrf0219801-13.CS.WB
Reference
Gopalakrishnan et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cnn 

Cnn

neutral component
cnn 

Cnn

neutral component
cnn 

Cnn

neutral component
Plp 

D-PLP

neutral component
Plp 

D-PLP

neutral component
Plp 

D-PLP

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; proteins produced from endogenous gene.

(Gopalakrishnan et al., 2012)

cnn and cp309 co-fractionate as part of a Sas-4-containing S-CAP complex (centrosomal scaffold).

(Gopalakrishnan et al., 2012)

Sas-4 complexes were immunoprecipitated from "high-speed lysates" depleted of mature centrosomes and different Sas-4-containing complexes were fractionated on a sucrose gradient.

(Gopalakrishnan et al., 2012)
FBrf0228958-1.Y2H
Reference
Lerit et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cnn 

Cnn

bait

fused to GAL4 DNA-binding domain

cnn 
cnn 
cnn 
cnn 
Plp 

D-Plp

prey

fused to GAL4 activation domain

Plp 
Plp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Plp 
middle region
sufficient binding region
aa 584-1301

construct "F2A", coordinates relative to Plp-PF

cnn 
Conserved Motif 2
sufficient binding region
aa 1087-1148

construct "CM2", coordinates relative to cnn-PA

cnn 
Conserved Motif 2
necessary binding region
aa 1087-1148

construct "CM2", coordinates relative to cnn-PA

cnn 
Conserved Motif 2 residue R1141
mutation disrupting interaction
aa 1141

R1141H, coordinates relative to cnn-PA

Plp 
C-terminal region
sufficient binding region
aa 2539-2895

construct "F5", coordinates relative to Plp-PF

cnn 
N-terminal region
sufficient binding region
aa 1-365

construct "F1", coordinates relative to cnn-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Lerit et al., 2015)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Lerit et al., 2015)

Two interaction interfaces were observed between Plp and cnn. Interaction 1 - Plp middle region binds to cnn C-terminal "Conserved Motif 2". Interaction 2 - Plp C-terminus binds to cnn N-terminus.

(Lerit et al., 2015)
FBrf0229262-3.Y2H
Reference
Richens et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cnn 

Cnn

bait

fused to GAL4 DNA-binding domain

cnn 
Plp 

D-Plp

prey

fused to GAL4 activation domain

Plp 
Plp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cnn 
C-terminal region
sufficient binding region
aa 602-1148

construct "C1/3", coordinates relative to cnn-PA

Plp 
C-terminal region
sufficient binding region
aa 2255-2471

construct "C1", coordinates relative to Plp-PF

Plp 
middle region
sufficient binding region
aa 1196-1878

construct "M4", coordinates relative to Plp-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Richens et al., 2015)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Richens et al., 2015)
FBrf0229262-4.Y2H
Reference
Richens et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Plp 

D-Plp

bait

fused to GAL4 DNA-binding domain

Plp 
cnn 

Cnn

prey

fused to GAL4 activation domain

cnn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cnn 
N-terminal region
sufficient binding region
aa 1-608

construct "N2/3", coordinates relative to cnn-PA

Plp 
C-terminal region
sufficient binding region
aa 2465-2895

construct "C2-3", coordinates relative to Plp-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Richens et al., 2015)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Richens et al., 2015)
FBrf0233282-94.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cnn 

Cnn

bait

fused to GAL4 DNA-binding domain

cnn 
Plp 

PLP

prey

fused to GAL4 activation domain

Plp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cnn 
N-terminal region
sufficient binding region
aa 1-365

coordinates relative to cnn-PA

Plp 
C-terminal region
sufficient binding region
aa 2539-2895

coordinates relative to Plp-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)
FBrf0233282-95.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Plp 

PLP

bait

fused to GAL4 DNA-binding domain

Plp 
cnn 

Cnn

prey

fused to GAL4 activation domain

cnn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Plp 
C-terminal region
sufficient binding region
aa 2539-2895

coordinates relative to Plp-PF

cnn 
N-terminal region
sufficient binding region
aa 1-365

coordinates relative to cnn-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)
FBrf0237751-2.Y2H
Reference
Citron et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Plp 

PLP

bait

fused to GAL4 DNA-binding domain

Plp 
cnn 

CNN

prey

fused to GAL4 activation domain

cnn 
cnn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cnn 
CM2 domain
sufficient binding region
aa 1087-1148

coordinates relative to cnn-PA

Plp 
interaction domain
sufficient binding region
aa 583-740

coordinates relative to Plp-PF

cnn 
CM2 domain residues 1131,1137
mutation disrupting interaction
aa 1131,1137

L1131A,L1137A, coordinates relative to cnn-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Citron et al., 2018)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Citron et al., 2018)
FBrf0237751-5.NMR
Reference
Citron et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cnn 

Cnn

neutral component
cnn 

Cnn

neutral component
cnn 
cnn 

Cnn

neutral component
cnn 
Plp 

PLP

neutral component
Plp 
Plp 

PLP

neutral component
Plp 

PLP

neutral component
Plp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cnn 
CM2 domain
sufficient binding region
aa 1090-1148

coordinates relative to cnn-PA

Plp 
interaction domain
sufficient binding region
aa 583-740

coordinates relative to Plp-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

(Citron et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (7)