FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mira, pros
FlyBase ID
FBig0000095500
Interaction Network
Interactions Browser links
mira network
pros network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0098349-1.Y2H
Reference
Shen et al., 1997
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 6embryonic stage 15late extended germ band stageembryonic stage 12embryonic stage 11embryonic stage 8embryonic stage 9early extended germ band stageembryonic stage 13embryonic stage 14embryonic stage 10extended germ band stageembryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pros 

Prospero

bait

fused to LexA DNA-binding domain

pros 
mira 

miranda

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pros 
asymmetric localization domain
sufficient binding region
aa 820-1026
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Shen et al., 1997)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Shen et al., 1997)
FBrf0098349-2.PD.A
Reference
Shen et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pros 

Pros

bait

GST tag

pros 
mira 

Miranda

prey

[35]S label

mira 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pros 
asymmetric localization domain
sufficient binding region
aa 820-1026
mira 
C-terminal region
sufficient binding region
aa 300-830
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Shen et al., 1997)
FBrf0099904-1.Y2H
Reference
Ikeshima-Kataoka et al., 1997
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pros 

Pros

bait

fused to GAL4 DNA-binding domain

pros 
mira 

Miranda

prey

fused to GAL4 activation domain

mira 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pros 
asymmetry domain
necessary binding region
QADSVTAAAEQLNKDLLLASQILDRKSPRTK
mira 
C-terminal region
sufficient binding region
aa 547-830
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Ikeshima-Kataoka et al., 1997)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Ikeshima-Kataoka et al., 1997)
FBrf0103035-2.PD.A
Reference
Shen et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pros 

Pros

bait

GST tag

pros 
mira 

mira

prey

[35]S label

mira 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mira 
region
unspecified role
aa 468-638
pros 
asymmetric localization domain
unspecified role
aa 820-1026
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Prey derived from in vitro translation.

(Shen et al., 1998)

GST pull down.

(Shen et al., 1998)

mira protein binds insc, pros and numb proteins through distinct domains.

(Shen et al., 1998)

Bait expressed and purified from bacteria.

(Shen et al., 1998)
FBrf0155686-7.coIP.WB
Reference
Petritsch et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mira 

Miranda

bait
pros 

Prospero

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Petritsch et al., 2003)

Positive control.

(Petritsch et al., 2003)
FBrf0155686-8.coIP
Reference
Petritsch et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mira 

Miranda

bait
pros 

prospero

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Petritsch et al., 2003)

Positive control. Detection method for pros mRNA was not reported.

(Petritsch et al., 2003)
FBrf0206088-28.PD.WB
Reference
Wirtz-Peitz et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mira 

Miranda

bait

GST tag

pros 

Prospero

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryo extract.

(Wirtz-Peitz et al., 2008)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)