FlyBase Gene Report: Dmel\apolpp

General Information

Symbol

Dmel\apolpp

Species

D. melanogaster

Name

apolipophorin

Annotation Symbol

CG11064

Feature Type

protein_coding_gene

FlyBase ID

FBgn0087002

Gene Model Status

Current

Stock Availability

20 publicly available

Gene Summary

apolipophorin (apolpp) encodes an apolipoprotein of the ApoB family. It is complexed with lipids to assemble the major hemolymph lipoprotein, lipophorin. Lipophorin transports neutral lipids, phospholipids and sterols between tissues. Lipophorin also carries lipophilic hormones and signaling molecules. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

Rfabg, Lpp, RfaBp, lipophorin, ApoLII

Key Links

Genomic Location

Cytogenetic map

102F7-102F8

Sequence location

4:1,060,969..1,077,273 [+]

Recombination map

4-0

RefSeq locus

NC_004353 REGION:1060969..1077273

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

NCBI

UCSC

Ensembl

Function

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

enables fatty acid binding

inferred from direct assay

(Duncan et al., 1994)

enables heme binding

inferred from direct assay

(Duncan et al., 1999)

enables lipid transporter activity

inferred from mutant phenotype

(Palm et al., 2012)

inferred from direct assay

(Pho et al., 1996)

enables microtubule binding

inferred from direct assay

(Sisson et al., 2000)

enables retinol binding

inferred from direct assay

(Duncan et al., 1994)

enables signaling receptor binding

inferred from physical interaction with FLYBASE:ptc; FB:FBgn0003892

(Callejo et al., 2008)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables lipid transporter activity

inferred from biological aspect of ancestor with PANTHER:PTN001155829

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001747, InterPro:IPR009454, InterPro:IPR015255, InterPro:IPR015816, InterPro:IPR015817, InterPro:IPR015819

(InterPro Project Members, 2004-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in lipid transport involved in lipid storage

inferred from mutant phenotype

(Ugrankar et al., 2019)

involved_in lipid transport

inferred from mutant phenotype

(Palm et al., 2012, Panakova et al., 2005)

inferred from direct assay

(Pho et al., 1996)

involved_in lipoprotein transport

inferred from direct assay

(Panakova et al., 2005)

involved_in positive regulation of canonical Wnt signaling pathway

inferred from mutant phenotype

(Panakova et al., 2005)

involved_in positive regulation of smoothened signaling pathway

inferred from mutant phenotype

(Panakova et al., 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in lipid transport

inferred from electronic annotation with InterPro:IPR001747, InterPro:IPR009454, InterPro:IPR015255, InterPro:IPR015816, InterPro:IPR015819

(InterPro Project Members, 2004-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in basal part of cell

inferred from direct assay

(Palm et al., 2012)

located_in endocytic vesicle

inferred from direct assay

(Callejo et al., 2008)

located_in extracellular space

inferred from direct assay

(Palm et al., 2012)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in extracellular region

non-traceable author statement

(Kutty et al., 1996)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Protein Function (UniProtKB)

Constitutes the major component of lipophorin, which mediates transport for various types of lipids in hemolymph. Acts by forming lipoprotein particles that bind lipoproteins and lipids. Also involved in the transport of hydrophobic ligands like juvenile hormones, pheromone hydrocarbons and carotenoids. Required for morphogens wingless (wg) and hedgehog (hh) function, probably by acting as vehicles for the movement of wg and hh, explaining how covalently lipidated wg and hh can spread over long distances. May also be involved in transport and/or metabolism of heme. Involved in yolk granule formation (PubMed:33891588). May be a component of yolk incorporated into yolk granules via yl/yolkless-mediated endocytosis and the endolysosomal pathway (PubMed:33891588).

(UniProt, Q9V496)

Summary (Interactive Fly)

Lipophorin - bears lipid-linked morphogens on its surface - required for long-range signaling activity of Wingless and Hedgehog - lipophorin receptors recruit the lipoprotein Lipid Transfer Particle (LTP) to the plasma membrane to mediate lipid uptake

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\apolpp for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V496)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.47

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

apolpp-RA

FBtr0089188

NM_079895

10287

3351

apolpp-RB

FBtr0334627

NM_001272156

11356

3351

apolpp-RC

FBtr0345296

NM_001297791

10338

3351

apolpp-RD

FBtr0345297

NM_001297792

10412

3351

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

apolpp-PA

FBpp0088252

372.7

3351

8.09

FBpp0306689

372.7

3351

8.09

FBpp0311463

372.7

3351

8.09

FBpp0311464

372.7

3351

8.09

Q9V496

NP_001284721

AHN58222

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

3351 aa isoforms: apolpp-PA, apolpp-PB, apolpp-PC, apolpp-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with Nrx-1 (via cytoplasmic domain); the interaction supports apolpp/ApoLI protein stability.

(UniProt, Q9V496)

Post Translational Modification

May be modified covalently by lipidation.

Cleaved into 2 chains by furin protease. However, prevention of cleavage does not impair its function (By similarity).

(UniProt, Q9V496)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\apolpp using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.95

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 12

amnioserosa

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 12

amnioserosa

(Kutty et al., 1996)

embryonic stage 13 -- 16

embryonic/larval fat body

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 14

fat body primordium | lateral

(Kutty et al., 1996)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval fat body

(Palm et al., 2012)

Additional Descriptive Data

At embryonic stage 12, high levels of apolpp transcript accumulates in the amnioserosa. Starting at late stage 14, apolpp is expressed in lateral region of the fat body primordium.

(Kutty et al., 1996)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 12 -- 14

yolk sac

(Kutty et al., 1996)

embryonic stage 14

dorsal apodeme anlage

(Kutty et al., 1996)

ventral apodeme anlage

(Kutty et al., 1996)

muscle attachment site

(Kutty et al., 1996)

second instar larval stage

perineurium

(Brankatschk and Eaton, 2010)

embryonic/larval central nervous system

(Brankatschk and Eaton, 2010)

larval optic anlage

Comment: ApoLII

(Brankatschk and Eaton, 2010)

wandering third instar larval stage

embryonic/larval hemolymph

(Palm et al., 2012)

adult stage

adult fat body

(Baer et al., 2012)

adult posterior midgut

(Baer et al., 2012)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval hemolymph

Comment: component of hemolymph clot

(Scherfer et al., 2004)

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

hemolymph

(Rodríguez-Vázquez et al., 2015)

Additional Descriptive Data

apolpp localizes to the perineurium and CNS. The protein moiety of Lpp, apolipophorin, undergoes furin dependent cleavage, generating apolipophorin I (ApoLI) and apolipophorin II (ApoLII) both of which circulate on low-density lipophorin particles in the hemolymph. Both cross the perineurium and localize to different places in the larval brain (as shown with tagged proteins).

(Brankatschk and Eaton, 2010)

Isolated from a pullout assay for proteins contained in clots from larval hemolymph and identified by mass spec.

(Scherfer et al., 2004)

At embryonic stage 12, apolpp protein is dispersed throughout the yolk sac. At embryonic stage 14, apolpp protein is localized at the developing apodemes

(Kutty et al., 1996)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in basal part of cell

inferred from direct assay

(Palm et al., 2012)

located_in endocytic vesicle

inferred from direct assay

(Callejo et al., 2008)

located_in extracellular space

inferred from direct assay

(Palm et al., 2012)

Expression Deduced from Reporters

Reporter: P{Lpp-GAL4.B}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 14

yolk nucleus

(Palm et al., 2012)

larval stage

embryonic/larval fat body

(Van De Bor et al., 2015, Palm et al., 2012, Brankatschk and Eaton, 2010)

adult stage

adult fat body

(Van De Bor et al., 2015)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\apolpp in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\apolpp

View exonic expression by tissue for Dmel\apolpp

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0087002

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43827/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0087002

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0087002

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11064

Flygut - An atlas of the Drosophila adult midgut

FBgn0087002

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\apolpp

Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 12 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 16 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of apolpp

Transgenic constructs containing regulatory region of apolpp

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of larval stage, with Scer\GAL4^αTub84B.PL

apolpp^{RNAi.ApoLII.UAS}

lethal - all die before end of second instar larval stage, with Scer\GAL4^apolpp.PB

apolpp^VDRC.cUa

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

apolpp^GD3156

viable, with Scer\GAL4^Mef2.PR

apolpp^GD3156

viable, with Scer\GAL4^pnr-MD237

apolpp^GD3156

Sterility

Allele

female semi-fertile, with Scer\GAL4^Cg.PA

apolpp^KK106311

Other Phenotypes

Allele

abnormal mitotic cell cycle | larval stage, with Scer\GAL4^apolpp.PB

apolpp^VDRC.cUa

abnormal neuroanatomy | second instar larval stage, with Scer\GAL4^apolpp.PB

apolpp^VDRC.cUa

abnormal size | adult stage, with Scer\GAL4^eya.enh1+E+2

apolpp^HMS00265

decreased cell death | adult stage | female, with Scer\GAL4^Lsp2.3.1

apolpp^HMS00265

decreased fecundity | female, with Scer\GAL4^Lsp2.3.1

apolpp^HMS00265

increased cell death | adult stage | female, with Scer\GAL4^Lsp2.3.1

some die during larval stage, with Scer\GAL4^αTub84B.PL

apolpp^{RNAi.ApoLII.UAS}

some die during second instar larval stage, with Scer\GAL4^apolpp.PB

apolpp^VDRC.cUa

visible | adult stage, with Scer\GAL4^eya.enh1+E+2

apolpp^HMS00265

Phenotype manifest in

Allele

adult gut, with Scer\GAL4^NP0001

apolpp^HM05157

egg chamber, with Scer\GAL4^Cg.PA

apolpp^KK106311

egg chamber, with Scer\GAL4^Lsp2.3.1

embryonic/larval dorsal tracheal branch | larval stage, with Scer\GAL4^btl.PS

apolpp^HM05157

embryonic/larval fat body, with Scer\GAL4^Adh.PF

apolpp^{RNAi.ApoLII.UAS}

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^Adh.PF

apolpp^{RNAi.ApoLI.UAS}

embryonic/larval hemolymph | third instar larval stage, with Scer\GAL4^apolpp.PB

apolpp^{RNAi.ApoLI.UAS}

embryonic/larval midgut | third instar larval stage, with Scer\GAL4^Adh.PF

embryonic/larval optic lobe | second instar larval stage, with Scer\GAL4^apolpp.PB

apolpp^VDRC.cUa

eye, with Scer\GAL4^eya.enh1+E+2

apolpp^HMS00265

fat cell | third instar larval stage, with Scer\GAL4^DcG

apolpp^HM05157

female germline stem cell, with Scer\GAL4^Lsp2.3.1

germarium, with Scer\GAL4^Lsp2.3.1

apolpp^HMS00265

lipid droplet, with Scer\GAL4^Adh.PF

apolpp^{RNAi.ApoLII.UAS}

lipid droplet, with Scer\GAL4^NP0001

apolpp^HM05157

lipid droplet | third instar larval stage, with Scer\GAL4^DcG

apolpp^HM05157

neuropil | second instar larval stage, with Scer\GAL4^apolpp.PB

apolpp^VDRC.cUa

wing disc, with Scer\GAL4^Adh.PF

apolpp^{RNAi.ApoLII.UAS}

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (7)

Hsap\LOC400499

3 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (5)

Rnor\Apob

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (6)

Mmus\Apob

3 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (3)

Xtro\XB6038542

3 of 13

Yes

Xtro\vtga2

1 of 13

Xtro\vwf

1 of 13

Danio rerio (Zebrafish) (15)

Drer\apoba

4 of 14

Yes

Drer\apobb.1

4 of 14

Yes

Drer\apobb.2

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

1 of 14

Drer\si:dkeyp-106c3.1

1 of 14

Drer\sspo

1 of 14

Drer\vwf

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\vit-1

5 of 14

Yes

Cele\vit-3

5 of 14

Yes

Cele\vit-4

5 of 14

Yes

Cele\vit-5

5 of 14

Yes

Cele\vit-2

4 of 14

Cele\vit-6

4 of 14

Cele\pals-24

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (8)

Agam\AgaP_AGAP001826

11 of 12

Yes

3 of 12

3 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:apolpp. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (2)

Apoltp

7 of 13

cv-d

4 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

obesity and cardiomyocyte regulation of systemic lipid levels, APOB-related

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

apolpp^GD3156

model of congenital heart disease

CEA

(Theis et al., 2020)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Hsap\LOC400499

3 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

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Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

apolpp - Ace

cosedimentation, western blot

(Panakova et al., 2005)

apolpp - Adh

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Ald1

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Argk1

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - CG16826

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - CG31343

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - CG32473

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - CG34205

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - CG4390

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - CG4962

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - CG5500

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - CG5506

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Con

cosedimentation, western blot

(Panakova et al., 2005)

apolpp - Cpr64Ac

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - EbpIII

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Fas1

cosedimentation, western blot

(Panakova et al., 2005)

apolpp - Fer1HCH

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Fer2LCH

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Gapdh2

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Gpdh1

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Idgf4

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Lsp2

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Nplp2

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Nrx-1

anti bait coimmunoprecipitation, western blot, two hybrid, pull down

(Tian et al., 2013)

apolpp - Obp44a

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Obp56d

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Obp99c

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - PPO1

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - PPO2

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Pgam1

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Rab1

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

apolpp - Scp1

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Seipin

anti tag coimmunoprecipitation, peptide massfingerprinting

(Bi et al., 2014)

apolpp - Taf9

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Tsf1

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Vajk2

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Yp1

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Yp2

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - Yp3

experimental knowledge based

(Guruharsha et al., 2011)

apolpp - dally

cosedimentation, anti tag western blot, anti tag coimmunoprecipitation, western blot

(Eugster et al., 2007)

apolpp - dlp

anti tag coimmunoprecipitation, western blot, cosedimentation, anti tag western blot

(Eugster et al., 2007)

apolpp - hh

anti bait coimmunoprecipitation, anti tag western blot, western blot, cosedimentation

(Eugster et al., 2007, Panakova et al., 2005)

apolpp - klg

cosedimentation, western blot

(Panakova et al., 2005)

apolpp - ptc

anti tag coimmunoprecipitation, western blot

(Callejo et al., 2008)

apolpp - wg

anti bait coimmunoprecipitation, western blot

(Panakova et al., 2005)

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Interacts with Nrx-1 (via cytoplasmic domain); the interaction supports apolpp/ApoLI protein stability.

(UniProt, Q9V496 )

Linkouts

BioGRID - A database of protein and genetic interactions.

68653

DroID - A comprehensive database of gene and protein interactions.

FBgn0087002

MIST (protein-protein) - An integrated Molecular Interaction Database

43827

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

4-0

Cytogenetic map

102F7-102F8

Sequence location

4:1,060,969..1,077,273 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

102F7-102F8

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

102F-102F

(determined by in situ hybridisation)

(Kutty et al., 1996)

102F-102F

(Kutty et al., 1996)

Experimentally Determined Recombination Data

Location

4-0

(FlyBase, 2016)

(Kutty et al., 1996)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (20)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

94567

w^* P{Lpp-GAL4.B}1

97113

w¹¹¹⁸; P{UAS-apolpp.E}2

27749

y¹; Mi{ET1}apolpp^MB07263

32827

y¹; Mi{MIC}apolpp^MI02094

84317

w^*; P{UAS-GC3Ai}2; P{Lpp-GAL4.B}3

28946

y¹ v¹; P{TRiP.HM05157}attP2

97114

y¹ w¹¹¹⁸; P{UAS-apolpp.E}3/TM2

84297

w^*; P{UAS-CC3Ai}2; P{Lpp-GAL4.B}3/TM6B, Tb¹

84338

w^*; P{UAS-VC3Ai}2; P{Lpp-GAL4.B}3/TM6B, Tb¹

VDRC

v100944

P{KK106311}VIE-260B

v6878

w¹¹¹⁸; P{GD3156}v6878

v318255

PBac{fTRG00900.sfGFP-TVPTBF}VK00033

More stocks available...

Genomic Clones (22)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (346)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0087002

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0087002

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Kelly et al., 2022, Eugster et al., 2007)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

In vitro studies reveal RfaBp gene is transcriptionally regulated by retinoic acid.

(Samuel et al., 1998)

RfaBp levels are high in retinoid replete flies, nearly zero in deprived flies and high for retinoic acid supplemented medium. This is important evidence that RfaBp expression is controlled by retinoids and other transcription activators.

(Shim et al., 1997)

Isolated using a rat antibody from a head cDNA library.

(Kutty et al., 1996)

RfaBp encodes a retinoid- and fatty-acid binding glycoprotein that is a member of the proapolipophorin gene family. RfaBp encodes a 3351 amino acid protein. The protein is initially dispersed throughout the embryonic amnioserosa sac and later concentrates at skeletal muscle epidermis apodemeal contact junctions during larval development.

(Kutty et al., 1996)

lipophorin protein has been purified and characterised.

(Pho et al., 1996)

There is preliminary evidence that retinoid (vitamin A) deprivation reduces RfaBp protein levels.

(Picking et al., 1996)

Vitamin A deprivation causes a reduction in the steady state level of retinoid binding protein, RfaBp.

(Picking et al., 1996)

Relationship to Other Genes

Source for database merge of

Source for merge of: RfaBp lipophorin

(Culi, 2007.10.12)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: apolpp Rfabg

(Culi, 2007.10.12)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (45)

Reported As

Symbol Synonym

APOL1

(Koehler and Huber, 2023)

ApoL1

(Reumer et al., 2009)

ApoL2

(Reumer et al., 2009)

ApoLI

(Bi et al., 2014, Brankatschk and Eaton, 2010, Eaton, 2009.8.6, Eugster et al., 2007, Panakova et al., 2005)

ApoLII

(Bi et al., 2014, Brankatschk and Eaton, 2010, Eaton, 2009.8.6, Hijazi et al., 2009, Eugster et al., 2007, Briscoe and Therond, 2005, Panakova et al., 2005)

ApoLPP

(Krejčová et al., 2023)

ApoLpp

(Ugrankar-Banerjee et al., 2023, Ugrankar et al., 2019)

Apolpp

(Rounds et al., 2022, Vásquez-Procopio et al., 2020, Hudson et al., 2019, Zhao and Karpac, 2017)

DRBP

(Picking et al., 1996)

LPP

(Lehmann, 2021, Meschi and Delanoue, 2021)

Lipophorin

(Eaton, 2009.8.6, Panakova and Eaton, 2006)

Lpp

(Zhao et al., 2023, Prince et al., 2020, Rommelaere et al., 2019, Franz et al., 2018, Staats et al., 2018, Hebbar et al., 2017, Matsuoka et al., 2017, Zhao and Karpac, 2017, Wong et al., 2016, Khaliullina et al., 2015, Bi et al., 2014, Palm et al., 2013, Baer et al., 2012, Brankatschk and Eaton, 2010, Khaliullina et al., 2009)

RFABG

(Tian et al., 2013, Vandenborre et al., 2010, Scherzer et al., 2003, Sisson et al., 2000, Vricella et al., 2000, Duncan et al., 1999, Kutty et al., 1999, Samuel et al., 1998, Shim et al., 1997, Kutty, 1996.7.3, Kutty et al., 1996)

RFABP

(Shibata et al., 2010)

RFBP

(Mann et al., 1999)

RfaBP

(Ballard et al., 2010, Podemski et al., 2004, Locke et al., 2000)

RfaBp

(Awofala et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Ballard et al., 2010, Müller et al., 2010, Deng et al., 2009, Reumer et al., 2009, Callejo et al., 2008, Riddle et al., 2008, Maines et al., 2007, Beller et al., 2006, Kim et al., 2005)

Rfabg

(Yang et al., 2024, Hope et al., 2020, Liu et al., 2017, Zhao and Karpac, 2017, Sarov et al., 2016, Ashwal-Fluss et al., 2014, Bi et al., 2014, Stern et al., 2014, Westholm et al., 2014, Chen et al., 2012, Riddle et al., 2012, Toku et al., 2011, Kallappagoudar et al., 2010, Kong et al., 2010, Wasbrough et al., 2010, Sharma and Singh, 2009, Fisher et al., 2008)

Rfabp

(Koya and Meller, 2015)

(Vierstraete et al., 2004)

apoLI

(Bi et al., 2014, Palm et al., 2013)

apoLII

(Bi et al., 2014, Palm et al., 2013)

apoLPP

(Rodríguez-Vázquez et al., 2015)

apoLpp

(Peng et al., 2024, Deshpande et al., 2022, Ding et al., 2022, Ding et al., 2022, Ding et al., 2021, Moraes and Montagne, 2021, Ramond et al., 2020, Lee et al., 2017, Baer et al., 2012, Palm et al., 2012)

apolpp

(Carrera et al., 2024, Krejčová et al., 2024, Huang et al., 2023, Hudson et al., 2023, Deshpande et al., 2022, Fourth Chromosome Resource Project and Newfeld, 2022-, Gao et al., 2022, Wu et al., 2022, Bilder et al., 2021, Juarez-Carreño et al., 2021, Pang et al., 2021, Tanaka et al., 2021, Cho et al., 2020, Ghosh et al., 2020, Theis et al., 2020, Franchet et al., 2019, Gene Disruption Project members, 2018-, Palm et al., 2012)

chr4:1088013..1088173

(Will et al., 2013)

lipophorin

(Kounatidis and Ligoxygakis, 2012)

rfabg

(Li et al., 2015, Pospisilik et al., 2010)

Name Synonyms

Apolipophorin

(Chen et al., 2012)

Lipophorin

(Palm and Rodenfels, 2020, Khaliullina et al., 2015, Mbodj et al., 2013, Eugster et al., 2007, Panakova and Eaton, 2006)

Retinoid and Fatty Acid Binding Glycoprotein

(Shim et al., 1997)

Retinoid and fatty-acid binding protein

(Ballard et al., 2010)

Retinoid- and Fatty Acid-binding Glycoprotein

(Kutty et al., 1996)

Retinoid- and fatty acid-binding glycoprotein

Retinoid- and fatty acid-binding protein

(Reumer et al., 2009)

Retinoid- and fatty-acid binding protein

(Cammarato et al., 2011)

Retinoid-fatty acid-binding glycoprotein

(Zeng et al., 2015)

RfaBp

(Kong et al., 2010)

apolipophorin

(Solis et al., 2013, Culi, 2007.10.12)

apolipophorin I

(Panakova et al., 2005)

apolipophorin II

(Panakova et al., 2005)

apolipophorin-2

(Bilousov et al., 2012)

lipophorin

(Wong et al., 2016, Rodríguez-Vázquez et al., 2015, Theopold et al., 2014, Parra-Peralbo and Culi, 2011, Brankatschk and Eaton, 2010, Reumer et al., 2009, Coudreuse and Korswagen, 2007, Culi, 2007.10.12)

retinoid and fatty acid binding protein

(Culi, 2007.10.12)

retinoid- and fatty acid-binding gene

(Kutty, 1996.7.3)

retinoid- and fatty acid-binding glycoprotein

(Jaspers et al., 2012, Kutty et al., 1996)

retinoid-fatty acid binding protein

(Xiao et al., 2002)

Secondary FlyBase IDs

FBgn0016724
FBgn0021778

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 71 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43827

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V496

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V496

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

L0MPS3

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V496

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0087002

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43827/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0087002

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0087002

FlyMine - An integrated database for Drosophila genomics

FBgn0087002

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG11064

MARRVEL_MODEL - MARRVEL (model organism gene)

43827

Linkouts

BioGRID - A database of protein and genetic interactions.

68653

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0087002

DroID - A comprehensive database of gene and protein interactions.

FBgn0087002

DRSC - Results frm RNAi screens

FBgn0087002

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0087002

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11064

Flygut - An atlas of the Drosophila adult midgut

FBgn0087002

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC034740

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

43827

References (200)

The following external sites may use different assemblies or annotations than FlyBase.

-0.95

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