FB2024_04 , released June 25, 2024
Gene: Dmel\Apoltp
Open Close
General Information
Symbol
Dmel\Apoltp
Species
D. melanogaster
Name
Apolipoprotein lipid transfer particle
Annotation Symbol
CG15828
Feature Type
protein_coding_gene
FlyBase ID
FBgn0032136
Gene Model Status
Current
Stock Availability
13 publicly available
Gene Summary
Apolipoprotein lipid transfer particle (Apoltp) encodes a apolipoprotein of the ApoB family. It is assembled with lipids to form high density, low abundance lipoproteins named lipid transfer particles (LTP). LTP catalyzes the transfer of lipids from the gut to circulating lipophorin and from lipophorin to peripheral tissues, such as imaginal discs and ovaries. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

LTP

Key Links
Genomic Location
Cytogenetic map
30C2-30C2
Sequence location
Recombination map
2-36
RefSeq locus
NT_033779 REGION:9634179..9656026
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (9 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables lipid transporter activity
inferred from mutant phenotype
(Palm et al., 2012)
enables lipoprotein particle receptor binding
inferred from physical interaction with FLYBASE:LpR2; FB:FBgn0051092
(Rodríguez-Vázquez et al., 2015)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables lipid transporter activity
inferred from biological aspect of ancestor with PANTHER:PTN001155829
(GO Reference Genome Project, 2011-)
(InterPro Project Members, 2004-)
Biological Process (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in acylglycerol transport
inferred from mutant phenotype
(Palm et al., 2012)
involved_in positive regulation of lipid transport
inferred from mutant phenotype
(Rodríguez-Vázquez et al., 2015)
involved_in sterol transport
inferred from mutant phenotype
(Palm et al., 2012)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in lipid transport
(InterPro Project Members, 2004-)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in basal part of cell
inferred from direct assay
(Palm et al., 2012)
located_in extracellular space
inferred from direct assay
(Palm et al., 2012)
located_in plasma membrane
inferred from direct assay
(Rodríguez-Vázquez et al., 2015)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Apolipoprotein lipid transfer particle (Apoltp) encodes a apolipoprotein of the ApoB family. It is assembled with lipids to form high density, low abundance lipoproteins named lipid transfer particles (LTP). LTP catalyzes the transfer of lipids from the gut to circulating lipophorin and from lipophorin to peripheral tissues, such as imaginal discs and ovaries. [Date last reviewed: 2019-03-07]
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Apoltp for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q7KTG2)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Apoltp-RC
FBtr0303874
NM_205948
13330
4333
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Apoltp-PC
FBpp0292877
494.3
4333
6.28
Q7KTG2
NP_995670
AAS64667
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Apoltp using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.85

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 11 -- 12
fat body/gonad primordium
(Fisher et al., 2012)
yolk nucleus
(Fisher et al., 2012)
embryonic stage 13 -- 16
amnioserosa
(Fisher et al., 2012)
embryonic/larval fat body
(Fisher et al., 2012)
yolk nucleus
(Fisher et al., 2012)
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage
embryonic/larval fat body
(Palm et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage
embryonic/larval hemolymph
(Palm et al., 2012)
wing pouch
(Rodríguez-Vázquez et al., 2015)
salivary gland imaginal ring
(Rodríguez-Vázquez et al., 2015)
embryonic/larval oenocyte
(Rodríguez-Vázquez et al., 2015)
embryonic/larval fat body
(Rodríguez-Vázquez et al., 2015)
gastric caecum
(Rodríguez-Vázquez et al., 2015)
ring gland
(Rodríguez-Vázquez et al., 2015)
adult stage
adult fat body
(Baer et al., 2012)
adult posterior midgut
(Baer et al., 2012)
oogenesis stage S8 -- S10
nurse cell
(Rodríguez-Vázquez et al., 2015)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 5 of adulthood & male -- day 7 of adulthood & male
spermatozoon
(Wasbrough et al., 2010)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage
hemolymph
(Rodríguez-Vázquez et al., 2015)
Additional Descriptive Data

Apoltp protein accumulates at the surface of nurse cells during oogenesis. Significant accumulation begins at oogenesis stage 9 and peaks at stage 10. In the wing pouch, Apoltp accumulates in basolateral cell membranes, and in a few apical vesicles. Larval expression is also observed in the salivary gland imaginal ring, the gastric caecum, the ring gland, oenocytes, and the fat body.

(Rodríguez-Vázquez et al., 2015)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in basal part of cell
inferred from direct assay
(Palm et al., 2012)
located_in extracellular space
inferred from direct assay
(Palm et al., 2012)
located_in plasma membrane
inferred from direct assay
(Rodríguez-Vázquez et al., 2015)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Apoltp in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 7 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Apoltp
Transgenic constructs containing regulatory region of Apoltp
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(2L)Exel6024
(Ryder, 2004.4.26)
Inferred from location ( 0 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (7)
Hsap\LOC400499
8 of 14
Yes
Yes
Hsap\APOB
3 of 14
No
Yes
0  
Hsap\MUC2
1 of 14
No
No
Hsap\MUC5B
1 of 14
No
No
Hsap\MUC19
1 of 14
No
No
Hsap\OTOGL
1 of 14
No
No
Hsap\SSPOP
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (5)
Rnor\Apob
4 of 14
Yes
Yes
Rnor\Ccnf
1 of 14
No
No
Rnor\Fcgbp
1 of 14
No
No
Rnor\Muc2
1 of 14
No
No
Rnor\Muc5b
1 of 14
No
No
Mus musculus (laboratory mouse) (6)
Mmus\Apob
5 of 14
Yes
Yes
Mmus\Fcgbp
1 of 14
No
No
Mmus\Muc2
1 of 14
No
No
Mmus\Muc5b
1 of 14
No
No
Mmus\Otogl
1 of 14
No
No
Mmus\Vwf
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (3)
Xtro\XB6038542
7 of 13
Yes
Yes
Xtro\vtga2
1 of 13
No
No
Xtro\vwf
1 of 13
No
No
Danio rerio (Zebrafish) (15)
Drer\apoba
5 of 14
Yes
Yes
Drer\apobb.1
5 of 14
Yes
Yes
Drer\apobb.2
5 of 14
Yes
Yes
Drer\si:dkeyp-106c3.1
4 of 14
No
Yes
Drer\vtg1
4 of 14
No
No
Drer\vtg2
4 of 14
No
No
Drer\vtg4
4 of 14
No
No
Drer\vtg5
4 of 14
No
No
Drer\vtg6
4 of 14
No
No
Drer\vtg7
4 of 14
No
No
Drer\vtg3
3 of 14
No
No
Drer\vtg8
3 of 14
No
No
Drer\si:dkey-65b12.6
1 of 14
No
No
Drer\sspo
1 of 14
No
No
Drer\vwf
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (6)
Cele\vit-1
4 of 14
Yes
No
Cele\vit-3
4 of 14
Yes
No
Cele\vit-4
4 of 14
Yes
No
Cele\vit-5
4 of 14
Yes
No
Cele\vit-2
3 of 14
No
No
Cele\vit-6
3 of 14
No
No
Anopheles gambiae (African malaria mosquito) (9)
Agam\AgaP_AGAP008807
12 of 12
Yes
Yes
Agam\AgaP_AGAP008810
8 of 12
No
Yes
Agam\AgaP_AGAP001826
2 of 12
No
No
Agam\AgaP_AGAP002032
2 of 12
No
No
Agam\AgaP_AGAP008369
2 of 12
No
No
Agam\AgaP_AGAP008809
2 of 12
No
Yes
Agam\AgaP_AGAP004203
1 of 12
No
No
Agam\AgaP_AGAP013109
1 of 12
No
No
Agam\AgaP_AGAP013503
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Apoltp. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (2)
apolpp
7 of 13
cv-d
4 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    APOB; apolipoprotein B
    model of  familial hypobetalipoproteinemia 1
    IEA
    (FlyBase, 2019-)
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Hsap\LOC400499
    8 of 14
        APOB; apolipoprotein B
        3 of 14
        107730
         
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Show/hide secondary interactors 
        (data from AllianceMine provided by esyN)
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Other Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        Apoltp - LpR2
        anti tag coimmunoprecipitation, Identification by mass spectrometry, western blot
        (Rodríguez-Vázquez et al., 2015)
        Summary of Genetic Interactions
        esyN Network Diagram
        Other Interaction Browsers
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2L
        Recombination map
        2-36
        Cytogenetic map
        30C2-30C2
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        30C2-30C2
        Limits computationally determined from genome sequence between P{EP}EP361&P{EP}peloEP2160 and P{lacW}zf30Ck02506&P{EP}undEP424
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        2-36

        (FlyBase, 2016)

        2-31.3

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (13)
        Genomic Clones (33)
        cDNA Clones (25)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        Antibody Information
        Laboratory Generated Antibodies
         

        polyclonal

        (Palm et al., 2012)
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments

            Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days.

            (Claridge-Chang et al., 2001)
            Relationship to Other Genes
            Source for database merge of
            Additional comments
            Nomenclature History
            Source for database identify of
            Nomenclature comments
            Etymology
            Synonyms and Secondary IDs (14)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 40 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            FlyMine - An integrated database for Drosophila genomics
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            MIST (protein-protein) - An integrated Molecular Interaction Database
            References (70)