FB2024_02 , released April 23, 2024
Gene: Dmel\ScsβA
Open Close
General Information
Symbol
Dmel\ScsβA
Species
D. melanogaster
Name
Succinyl-coenzyme A synthetase β subunit, ADP-forming
Annotation Symbol
CG11963
Feature Type
FlyBase ID
FBgn0037643
Gene Model Status
Stock Availability
Enzyme Name (EC)
succinate--CoA ligase (ADP-forming) (6.2.1.5)
Gene Summary
Succinyl-coenzyme A synthetase β subunit, ADP-forming (ScsβA) encodes a subunit of the ligase that converts succinate to succinyl-CoA in the TCA/Krebs cycle. It contributes to apoptosis and sperm individualization. [Date last reviewed: 2019-09-12] (FlyBase Gene Snapshot)
Also Known As

skap, SkpA Associated Protein, A-Sβ

Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
3-48
RefSeq locus
NT_033777 REGION:8938724..8943027
Sequence
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
Gene Ontology (GO) Annotations (12 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR011761, InterPro:IPR013815
inferred from electronic annotation with InterPro:IPR005811
inferred from electronic annotation with InterPro:IPR011761
inferred from sequence or structural similarity with UniProtKB:F1LM47
inferred from biological aspect of ancestor with PANTHER:PTN000212531
Biological Process (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from mutant phenotype
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000212531
inferred from electronic annotation with InterPro:IPR005809, InterPro:IPR034723
inferred from biological aspect of ancestor with PANTHER:PTN000212531
inferred from sequence or structural similarity with UniProtKB:F1LM47
Cellular Component (4 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from direct assay
located_in mitochondrion
inferred from high throughput direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
is_active_in mitochondrion
inferred from biological aspect of ancestor with PANTHER:PTN000212530
located_in mitochondrion
inferred from electronic annotation with InterPro:IPR034723
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000212531
Gene Group (FlyBase)
Protein Family (UniProt)
-
Catalytic Activity (EC/Rhea)
succinate-CoA ligase (ADP-forming) activity
ATP + CoA + succinate = ADP + phosphate + succinyl-CoA (6.2.1.5)
RHEA 17661:
Summaries
Gene Snapshot
Succinyl-coenzyme A synthetase β subunit, ADP-forming (ScsβA) encodes a subunit of the ligase that converts succinate to succinyl-CoA in the TCA/Krebs cycle. It contributes to apoptosis and sperm individualization. [Date last reviewed: 2019-09-12]
Gene Group (FlyBase)
SUCCINYL-COA SYNTHETASE SUBUNITS -
Succinyl-CoA synthetases are mitochondrial enzymes that catalyze the reversible conversion of succinyl-CoA to succinate in the only step of the tricarboxylic acid cycle coupled with substrate-level phosphorylation of either ADP or GDP. In eukaryotes, the functional enzyme is a heterodimeric complex composed of a single alpha subunit and one of two isoforms of a beta subunit that determines the substrate specificity, resulting in an ATP- or GTP-generating enzyme. (Adapted from PMID:37445899.)
Gene Model and Products
Number of Transcripts
8
Number of Unique Polypeptides
4

Please see the JBrowse view of Dmel\ScsβA for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q95U38)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Gene model reviewed during 6.04

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0081957
1736
502
FBtr0113206
1764
502
FBtr0301786
1767
503
FBtr0301787
1739
503
FBtr0334327
1901
451
FBtr0334328
1873
451
FBtr0347567
1876
452
FBtr0347568
1904
452
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
FBpp0081437
54.8
502
9.05
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

502 aa isoforms: ScsβA-PA, ScsβA-PB
503 aa isoforms: ScsβA-PC, ScsβA-PD
452 aa isoforms: ScsβA-PI, ScsβA-PJ
451 aa isoforms: ScsβA-PG, ScsβA-PH
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ScsβA using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.34

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

ScsbetaA immunoreactivity has a punctate distribution in the cytosol of larval neuroblasts, where it colocalizes with sea protein.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
located_in mitochondrion
inferred from high throughput direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ScsβA in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of ScsβA
Transgenic constructs containing regulatory region of ScsβA
Aberrations (Deficiencies and Duplications) ( 2 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (3)
12 of 14
Yes
Yes
5 of 14
No
No
2 of 14
No
No
1  
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (3)
13 of 14
Yes
Yes
5 of 14
No
No
2 of 14
No
No
Mus musculus (laboratory mouse) (3)
13 of 14
Yes
Yes
4 of 14
No
No
2 of 14
No
No
Xenopus tropicalis (Western clawed frog) (2)
8 of 13
Yes
Yes
4 of 13
No
No
Danio rerio (Zebrafish) (4)
13 of 14
Yes
Yes
5 of 14
No
No
2 of 14
No
No
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (4)
13 of 14
Yes
Yes
5 of 14
No
No
2 of 14
No
No
2 of 14
No
No
Anopheles gambiae (African malaria mosquito) (4)
12 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (6)
12 of 13
Yes
Yes
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (1)
12 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (3)
11 of 12
Yes
Yes
2 of 12
No
No
1 of 12
No
No
Escherichia coli (enterobacterium) (2)
10 of 11
Yes
Yes
1 of 11
No
No
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:ScsβA. Refer to their site for version information.
Paralogs
Paralogs (via DIOPT v9.1)
Drosophila melanogaster (Fruit fly) (2)
9 of 13
2 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Show/hide secondary interactors 
    (data from AllianceMine provided by esyN)
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Other Interaction Browsers

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    Show/hide secondary interactors 
    (data from AllianceMine provided by esyN)
    esyN Network Key:
    Suppression
    Enhancement
    Other Interaction Browsers

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
    KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
    Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
    External Data
    Linkouts
    KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map
    3-48
    Cytogenetic map
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    85B7-85B7
    Limits computationally determined from genome sequence between P{lacW}l(3)L4740L4740 and P{EP}D1EP473
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (10)
    Genomic Clones (18)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (149)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Cell Line Information
    Publicly Available Cell Lines
     
      Other Stable Cell Lines
       
        Other Comments
        Relationship to Other Genes
        Source for database merge of
        Additional comments
        Nomenclature History
        Source for database identify of

        Source for identity of: skap CG11963

        Source for identity of: ScsβA skap

        Nomenclature comments
        Etymology
        Synonyms and Secondary IDs (16)
        Reported As
        Name Synonyms
        ATP-specific SCSβ
        ATP-specific form of the succinyl-CoA synthetase β subunit
        SkpA Associated Protein
        SkpA-associated protein
        Succinyl-coenzyme A synthetase β subunit, ADP-forming
        skpA associated protein
        skpA-associated protein
        succinyl CoA synthetase
        Secondary FlyBase IDs
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          Study result (0)
          Result
          Result Type
          Title
          External Crossreferences and Linkouts ( 75 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
          EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
          FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
          FlyMine - An integrated database for Drosophila genomics
          KEGG Genes - Molecular building blocks of life in the genomic space.
          MARRVEL_MODEL - MARRVEL (model organism gene)
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
          KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
          References (84)