FlyBase Gene Report: Dmel\Plod

(Lee and Thomas, 2011)

(Bunt et al., 2011)

embryonic stage 13 -- 16

(Fisher et al., 2012)

embryonic stage 13 -- 17

(Lee and Thomas, 2011)

fat body primordium

(Bunt et al., 2011)

oogenesis stage S7 -- S8 & adult stage | female

follicle cell

(Lerner et al., 2013)

nurse cell

(Lerner et al., 2013)

Additional Descriptive Data

Plod transcript is not detected until embryonic stage 12. Plod transcript expression is high in hemocytes and fat bodies at embryonic stages 15-17.

(Bunt et al., 2011)

Little or no Plod expression is observed in early embryos. Plod transcripts are detected in hemocytes starting in embryonic stage 12 and continuing throughout embryogenesis. Expression is found in hemocytes populating the whole embryo including those in the head and surrounding the CNS. Expression in fat body is seen at stage 13 and occurs at high levels in later stages.

(Lee and Thomas, 2011)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 12 -- 17

(Lee and Thomas, 2011)

(Bunt et al., 2011)

embryonic stage 13 -- 17

(Lee and Thomas, 2011)

fat body primordium

(Bunt et al., 2011)

embryonic stage 16

(Lee and Thomas, 2011)

embryonic stage 16 -- 17

(Bunt et al., 2011)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Plod protein is not detected until embryonic stage 12. Plod protein expression is high in hemocytes and fat bodies at embryonic stages 15-17. Expression is also observed in the anal pads of late-stage embryos.

(Bunt et al., 2011)

No Plod protein is observed in early embryos. Plod protein is detected in hemocytes starting in embryonic stage 12 and continuing throughout embryogenesis. Expression is found in hemocytes throughout the embryo. Expression in fat body is seen from stage 13 and continues throughout embryogenesis. High levels of Plod protein are observed in anal pads in late embryos.

(Lee and Thomas, 2011)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in endoplasmic reticulum

inferred from direct assay

(Bunt et al., 2011)

located_in extracellular space

inferred from direct assay

(Bunt et al., 2011)

located_in perinuclear region of cytoplasm

inferred from direct assay

(Bunt et al., 2011)

Expression Deduced from Reporters

Reporter: P{GawB}Plod^NP5633

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 15 -- 17

fat body primordium

(Bunt et al., 2011)

embryonic stage 16

(Lee and Thomas, 2011)

(Lee and Thomas, 2011)

(Lee and Thomas, 2011)

embryonic stage 16 -- 17

(Bunt et al., 2011)

View Dmel\Plod in JBrowse

(Bunt et al., 2011)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Plod

View exonic expression by tissue for Dmel\Plod

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0036147

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39265/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0036147

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0036147

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0036147

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6199

Flygut - An atlas of the Drosophila adult midgut

FBgn0036147

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Plod

Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Plod

Transgenic constructs containing regulatory region of Plod

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Plod^EY11195

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Plod^GD5882

viable, with Scer\GAL4^Mef2.PR

Plod^GD5882

viable, with Scer\GAL4^pnr-MD237

Plod^GD5882

viable, with Scer\GAL4^tin.CΔ4

Plod^GD5882

Sterility

Allele

fertile

Plod^EY11195

Phenotype manifest in

Allele

egg

Plod^N26-5

egg, with Scer\GAL4^NP1624

Plod^GD5882

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

Plod^HMS01259

extracellular matrix | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

Plod^HMS01259

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (7)

Hsap\PLOD1

13 of 14

Yes

Hsap\PLOD3

13 of 14

Yes

Hsap\PLOD2

12 of 14

Yes

Hsap\OGFOD2

2 of 14

Yes

Hsap\CERCAM

1 of 14

Hsap\COLGALT1

1 of 14

Hsap\COLGALT2

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (7)

Rnor\Plod1

13 of 14

Yes

Rnor\Plod3

13 of 14

Yes

Rnor\Plod2

12 of 14

Yes

Rnor\Ogfod2

2 of 14

Yes

Rnor\Cercam

1 of 14

Rnor\Colgalt1

1 of 14

Rnor\Colgalt2

1 of 14

Mus musculus (laboratory mouse) (7)

Mmus\Plod3

13 of 14

Yes

Mmus\Plod1

12 of 14

Yes

Mmus\Plod2

12 of 14

Yes

Mmus\Ogfod2

2 of 14

Yes

Mmus\Cercam

1 of 14

Mmus\Colgalt1

1 of 14

Mmus\Colgalt2

1 of 14

Xenopus tropicalis (Western clawed frog) (8)

Xtro\plod2

9 of 13

Yes

Xtro\plod3

9 of 13

Yes

Xtro\plod1

7 of 13

Yes

Xtro\lhfpl4

1 of 13

Xtro\ogfod2

1 of 13

Yes

Xtro\pcdhgc4

1 of 13

Yes

Xtro\ufm1

1 of 13

Xtro\uqcrc2

1 of 13

Danio rerio (Zebrafish) (9)

Drer\plod1a

13 of 14

Yes

Drer\plod3

13 of 14

Yes

Drer\plod2

12 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\let-268

14 of 14

Yes

Cele\D2045.9

1 of 14

Anopheles gambiae (African malaria mosquito) (3)

Agam\AgaP_AGAP001507

12 of 12

Yes

Agam\AgaP_AGAP012933

1 of 12

Agam\GLT25_ANOGA

1 of 12

Arabidopsis thaliana (thale-cress) (6)

Atha\AT1G48740

3 of 13

Yes

Atha\AT5G43660

3 of 13

Yes

Atha\AT1G48700

2 of 13

Yes

Atha\AT3G18210

2 of 13

Yes

Atha\AT1G22950

1 of 13

Yes

Atha\AT1G47940

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Plod. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

CG31915

3 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

PLOD1; procollagen-lysine,2-oxoglutarate 5-dioxygenase 1

model of Ehlers-Danlos syndrome kyphoscoliotic type 1

IEA

(FlyBase, 2019-)

PLOD2; procollagen-lysine,2-oxoglutarate 5-dioxygenase 2

model of Bruck syndrome

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PLOD1; procollagen-lysine,2-oxoglutarate 5-dioxygenase 1

13 of 14

153454

EHLERS-DANLOS SYNDROME, KYPHOSCOLIOTIC TYPE, 1; EDSKSCL1

Ehlers-Danlos syndrome kyphoscoliotic type 1

PLOD3; procollagen-lysine,2-oxoglutarate 5-dioxygenase 3

13 of 14

603066

BONE FRAGILITY WITH CONTRACTURES, ARTERIAL RUPTURE, AND DEAFNESS; BCARD

PLOD2; procollagen-lysine,2-oxoglutarate 5-dioxygenase 2

12 of 14

601865

BRUCK SYNDROME 2; BRKS2

Bruck syndrome

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

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(data from AllianceMine provided by esyN)

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Circle

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Legend:

Protein

RNA

Selected Interactor(s)

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

MIST Molecular Interaction Search Tool

View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Plod - Agbe

(Guruharsha et al., 2011)

Plod - CG4872

(Guruharsha et al., 2011)

Plod - CG7049

(Guruharsha et al., 2011)

Plod - Col4a1

(Guruharsha et al., 2011)

Plod - Hsp68

(Guruharsha et al., 2011)

Plod - Swip-1

(Guruharsha et al., 2011)

Plod - Tep4