FlyBase Gene Report: Dmel\Or49a

General Information

Symbol

Dmel\Or49a

Species

D. melanogaster

Name

Odorant receptor 49a

Annotation Symbol

CG13158

Feature Type

protein_coding_gene

FlyBase ID

FBgn0033727

Gene Model Status

Current

Stock Availability

6 publicly available

Gene Summary

Odorant receptor 49a (Or49a) encodes a multi-transmembrane chemoreceptor that mediates response to volatile chemicals. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

49a

Key Links

Genomic Location

Cytogenetic map

49A2-49A2

Sequence location

2R:12,393,483..12,394,842 [+]

Recombination map

2-67

RefSeq locus

NT_033778 REGION:12393483..12394842

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (10 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ionotropic olfactory receptor activity

inferred from sequence or structural similarity with UniProtKB:Q9VHQ7

(Gene Ontology Curators, 2002-)

enables odorant binding

inferred from electronic annotation with InterPro:IPR004117

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with FLYBASE:Or43b; FB:FBgn0026393

(Gene Ontology Curators, 2002-)

inferred from sequence or structural similarity with UniProtKB:Q9VHQ7

(Gene Ontology Curators, 2002-)

enables olfactory receptor activity

inferred from electronic annotation with InterPro:IPR004117

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000474402

(GO Reference Genome Project, 2011-)

non-traceable author statement

(Gao and Chess, 1999)

Biological Process (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in detection of chemical stimulus involved in sensory perception of smell

inferred from mutant phenotype

(Kreher et al., 2005)

involved_in olfactory behavior

inferred from mutant phenotype

(Fishilevich et al., 2005)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in detection of chemical stimulus involved in sensory perception of smell

inferred from biological aspect of ancestor with PANTHER:PTN000474402

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:Q9VHQ7

(Gene Ontology Curators, 2002-)

involved_in sensory perception of smell

non-traceable author statement

(Gao and Chess, 1999)

inferred from electronic annotation with InterPro:IPR004117

(InterPro Project Members, 2004-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

part_of cation channel complex

inferred from sequence or structural similarity with UniProtKB:Q9VHQ7

(Gene Ontology Curators, 2002-)

located_in dendrite membrane

inferred from sequence or structural similarity with FLYBASE:Or22a; FB:FBgn0026398

(Gene Ontology Curators, 2002-)

located_in membrane

non-traceable author statement

(Gao and Chess, 1999)

inferred from electronic annotation with InterPro:IPR004117

(InterPro Project Members, 2004-)

is_active_in plasma membrane

inferred from sequence or structural similarity with UniProtKB:Q9VHQ7

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000474402

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

ODORANT RECEPTORS

Protein Family (UniProt)

Belongs to the insect chemoreceptor superfamily. Heteromeric odorant receptor channel (TC 1.A.69) family. Or49a subfamily. (Q9V6A9)

Protein Signatures (InterPro)

Olfactory receptor, insect

Summaries

Gene Snapshot

Odorant receptor 49a (Or49a) encodes a multi-transmembrane chemoreceptor that mediates response to volatile chemicals. [Date last reviewed: 2019-03-14]

Gene Group (FlyBase)

ODORANT RECEPTORS -: Insect odorant receptors (ORs) are a family of chemoreceptors activated by volatile chemicals. ORs form heteromeric complexes, believed to function as non-selective cation channels, comprising an OR and the co-receptor Orco. ORs are expressed in the dendrites of subsets of olfactory neurons in the antenna and maxillary palp. (Adapted from FBrf0168007 and FBrf0220463).

Protein Function (UniProtKB)

Odorant receptor which mediates acceptance or avoidance behavior, depending on its substrates. The odorant receptor repertoire encodes a large collection of odor stimuli that vary widely in identity, intensity, and duration. May form a complex with Orco to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability. Involved in the behavioral responses to butanol and 2-heptanone.

(UniProt, Q9V6A9)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Or49a for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V6A9)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene structure update based on personal communication to FlyBase by Eleanor Whitfield, July 26, 2002.

Gene structure based on FBrf0136024, personal communication to FlyBase from Hugh Robertson.

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Or49a-RA

FBtr0087918

NM_078987

1191

396

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Or49a-PA

FBpp0087029

46.5

396

9.20

Q9V6A9

NP_523711

AAF58519

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with Orco. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway (By similarity).

(UniProt, Q9V6A9)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Olfactory receptor, insect

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

AE013599 / AAF58519

UniProt

Q9V6A9

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Or49a using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

NA

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{Or49a-GAL4.F}

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

larval olfactory receptor neuron Or49a

(Masuda-Nakagawa et al., 2009)

larval antennal lobe glomerulus 49a

(Masuda-Nakagawa et al., 2009)

adult stage

antennal basiconic sensillum ab10

(Larter et al., 2016)

Reporter: P{Or49a-GAL4.K}

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

olfactory receptor neuron of dorsal organ

(Kreher et al., 2005)

olfactory receptor neuron of terminal organ | faint

(Kreher et al., 2005)

olfactory receptor neuron of embryonic/larval antennal lobe

(Kreher et al., 2005)

Reporter: P{Or49a-GAL4.L}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult olfactory receptor neuron Or49a/85f

(Li et al., 2013)

Reporter: P{Or49a-Mmus\Cd8a.GFP}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult olfactory receptor neuron Or49a/85f

(Couto et al., 2005)

antennal basiconic sensillum ab10

(Couto et al., 2005)

antennal lobe glomerulus DL4

(Couto et al., 2005)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Or49a in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Or49a

View exonic expression by tissue for Dmel\Or49a

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36350/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0033727

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0033727

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0033727

Flygut - An atlas of the Drosophila adult midgut

FBgn0033727

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Or49a

Transgenic constructs containing regulatory region of Or49a

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2R)Exel6061

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Or49a^e02161

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Or49a^GD743

viable, with Scer\GAL4^Mef2.PR

Or49a^GD743

viable, with Scer\GAL4^pnr-MD237

Or49a^GD743

Sterility

Allele

fertile

Or49a^e02161

Other Phenotypes

Allele

abnormal smell perception, with Scer\GAL4^Or22a.8197

Or49a^UAS.Tag:MYC

visible, with Scer\GAL4^pnr-MD237

Or49a^GD743

Phenotype manifest in

Allele

trichogen cell, with Scer\GAL4^pnr-MD237

Or49a^GD743

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (71)

Agam\GPROR8

5 of 12

Yes

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\GPROR61

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Or49a. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (59)

6 of 13

6 of 13

6 of 13

6 of 13

6 of 13

5 of 13

5 of 13

5 of 13

4 of 13

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

MIST Molecular Interaction Search Tool

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Interacts with Orco. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway (By similarity).

(UniProt, Q9V6A9 )

Linkouts

BioGRID - A database of protein and genetic interactions.

62127

DroID - A comprehensive database of gene and protein interactions.

FBgn0033727

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-67

Cytogenetic map

49A2-49A2

Sequence location

2R:12,393,483..12,394,842 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

49A2-49A2

Limits computationally determined from genome sequence between P{lacW}l(2)k11404^k11404 and P{lacW}fdl^k17040

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-67

(FlyBase, 2016)

2-68.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (6)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

9985

w^*; P{Or49a-GAL4.F}47.2A

18020

w¹¹¹⁸; PBac{RB}Or49a^e02161

64581

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05600}attP40

NIG-Fly

2RG-0316

y² VhaAC39-1^cho-1 v¹; P{U6-Or49a.NIG.sgRNA}attP40/CyO

VDRC

v108800

P{KK105568}VIE-260B

v24498

w¹¹¹⁸; P{GD743}v24498

Genomic Clones (10)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (0)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0033727

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0033727

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Or49a is necessary for larval chemotaxis to the odour 1-hexanol, but is sufficient only in concert with another olfactory sensory neuron.

(Fishilevich et al., 2005)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Or49a CG13158

(Warr and Vosshall, 2000.5.19)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

49A.1

(Warr and Vosshall, 2000.5.19)

49a

(Masuda-Nakagawa et al., 2009, Vogt et al., 2002)

CG13158

(National Institute of Genetics Fly Stocks, 2022-, Keleman et al., 2009.8.5, Perkins et al., 2009, Robertson, 2009, Whitfield, 2002.7.26)

DOR10

(Warr and Vosshall, 2000.5.19)

DmOr49a

(Haverkamp et al., 2018)

OR49a

(Walker et al., 2023, Das et al., 2019, Aranha and Vasconcelos, 2018, Störtkuhl and Fiala, 2011, Stocker, 2006)

Name Synonyms

Odorant receptor 49a

(Cho et al., 2005)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 21 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36350

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAF58519

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V6A9

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V6A9

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.