FB2024_04 , released June 25, 2024
Gene: Dmel\wash
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General Information
Symbol
Dmel\wash
Species
D. melanogaster
Name
washout
Annotation Symbol
CG13176
Feature Type
protein_coding_gene
FlyBase ID
FBgn0033692
Gene Model Status
Current
Stock Availability
17 publicly available
Gene Summary
washout (wash) encodes a Wiskott-Aldrich syndrome protein that polymerizes branched actin through activation of the Arp2/3 complex, as well as bundles and/or crosslinks actin and microtubules. It functions as a downstream effector of the product of Rho1 in a context dependent manner. It is involved in nuclear and cytoplasmic cytoskeleton related cellular processes, including ooplasmic streaming, endosome recycing, hemocyte migration, and global nuclear organization. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)
All Summaries
Also Known As

p63, p65

Key Links
Genomic Location
Cytogenetic map
48E6-48E6
Sequence location
Recombination map
2-66
RefSeq locus
NT_033778 REGION:12180493..12182373
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (35 terms)
Molecular Function (6 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
enables chromatin binding
inferred from direct assay
(Verboon et al., 2015)
enables GTPase binding
inferred from physical interaction with FLYBASE:Rho1; FB:FBgn0014020
(Liu et al., 2009)
enables lamin binding
inferred from physical interaction with FLYBASE:Lam; FB:FBgn0002525
(Verboon et al., 2015)
inferred from direct assay
(Verboon et al., 2020)
enables protein binding
inferred from physical interaction with FLYBASE:spir; FB:FBgn0003475
(Liu et al., 2009)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables alpha-tubulin binding
inferred from electronic annotation with InterPro:IPR028290
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN001154810
(GO Reference Genome Project, 2011-)
enables gamma-tubulin binding
inferred from biological aspect of ancestor with PANTHER:PTN001154810
(GO Reference Genome Project, 2011-)
Biological Process (20 terms)
Terms Based on Experimental Evidence (18 terms)
CV Term
Evidence
References
involved_in actin crosslink formation
inferred from direct assay
(Liu et al., 2009)
involved_in actin cytoskeleton organization
inferred from mutant phenotype
(Nagel et al., 2017)
involved_in actin filament bundle assembly
inferred from direct assay
(Liu et al., 2009)
involved_in Arp2/3 complex-mediated actin nucleation
inferred from direct assay
(Liu et al., 2009)
involved_in heterochromatin formation
inferred from mutant phenotype
(Verboon et al., 2015)
involved_in microtubule bundle formation
inferred from direct assay
(Liu et al., 2009)
involved_in nuclear envelope budding
inferred from mutant phenotype
(Verboon et al., 2020)
involved_in nuclear envelope organization
inferred from mutant phenotype
(Verboon et al., 2015)
involved_in nuclear inner membrane organization
inferred from mutant phenotype
(Verboon et al., 2020)
involved_in oogenesis
inferred from mutant phenotype
(Liu et al., 2009)
involved_in positive regulation of cell adhesion mediated by integrin
inferred from mutant phenotype
(Nagel et al., 2017)
involved_in prepupal development
inferred from mutant phenotype
(Linardopoulou et al., 2007)
involved_in regulation of chromosome organization
inferred from mutant phenotype
(Verboon et al., 2015)
involved_in regulation of endocytic recycling
inferred from mutant phenotype
(Nagel et al., 2017)
involved_in regulation of lysosomal lumen pH
inferred from mutant phenotype
(Nagel et al., 2017)
involved_in regulation of nucleus organization
inferred from mutant phenotype
(Verboon et al., 2015)
involved_in retrograde transport, endosome to Golgi
inferred from mutant phenotype
(Dong et al., 2013)
involved_in spiracle morphogenesis, open tracheal system
inferred from mutant phenotype
(Linardopoulou et al., 2007)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in Arp2/3 complex-mediated actin nucleation
inferred from biological aspect of ancestor with PANTHER:PTN001154810
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR028290
(InterPro Project Members, 2004-)
involved_in endocytic recycling
inferred from biological aspect of ancestor with PANTHER:PTN001154810
(GO Reference Genome Project, 2011-)
involved_in exocytosis
inferred from biological aspect of ancestor with PANTHER:PTN001154810
(GO Reference Genome Project, 2011-)
involved_in retrograde transport, endosome to Golgi
inferred from biological aspect of ancestor with PANTHER:PTN001154810
(GO Reference Genome Project, 2011-)
Cellular Component (9 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Verboon et al., 2015, Rodriguez-Mesa et al., 2012)
located_in endosome
inferred from direct assay
(Dong et al., 2013)
colocalizes_with nuclear envelope
inferred from direct assay
(Verboon et al., 2020)
located_in nuclear envelope
inferred from direct assay
(Verboon et al., 2015)
located_in nucleoplasm
inferred from direct assay
(Verboon et al., 2020)
located_in nucleus
inferred from direct assay
(Verboon et al., 2015, Rodriguez-Mesa et al., 2012)
located_in perinuclear region of cytoplasm
inferred from direct assay
(Rodriguez-Mesa et al., 2012)
part_of WASH complex
inferred from direct assay
(Jia et al., 2010)
inferred from direct assay
(Verboon et al., 2020)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
is_active_in early endosome
inferred from biological aspect of ancestor with PANTHER:PTN001154810
(GO Reference Genome Project, 2011-)
located_in early endosome
inferred from electronic annotation with InterPro:IPR028290
(InterPro Project Members, 2004-)
is_active_in recycling endosome
inferred from biological aspect of ancestor with PANTHER:PTN001154810
(GO Reference Genome Project, 2011-)
part_of WASH complex
inferred from biological aspect of ancestor with PANTHER:PTN001154810
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity with UniProtKB:A8K0Z3
(Derivery and Gautreau, 2010)
inferred from electronic annotation with InterPro:IPR028290
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the WASH1 family. (Q7JW27)
Protein Signatures (InterPro)
Summaries
Gene Snapshot
washout (wash) encodes a Wiskott-Aldrich syndrome protein that polymerizes branched actin through activation of the Arp2/3 complex, as well as bundles and/or crosslinks actin and microtubules. It functions as a downstream effector of the product of Rho1 in a context dependent manner. It is involved in nuclear and cytoplasmic cytoskeleton related cellular processes, including ooplasmic streaming, endosome recycing, hemocyte migration, and global nuclear organization. [Date last reviewed: 2019-03-21]
Gene Group (FlyBase)
WISKOTT-ALDRICH SYNDROME PROTEINS -
The Wiskott-Aldrich Syndrome Proteins (WASP) are a family of nucleation-promoting factors. WASP proteins share a conserved Arp2/3-complex-activating C-terminal VCA/WCA (verprolin/WASP, cofilin, acidic) domain. WASP members participate in cytoskeleton organization, notably through the stimulation of the actin-nucleating activity of the Arp2/3 complex. (Adapted from FBrf0230860 and FBrf0217509).
WASP AND SCAR HOMOLOG COMPLEX -
The WASP and SCAR Homologue complex (WASH-C) is a multi-subunit complex that activates Arp2/3-mediated actin nucleation. WASH-C contains the nucleation-promoting factor wash, a member of the WASP family. (Adapted from FBrf0232381 and PMID:19922875).
Protein Function (UniProtKB)
Acts as a nucleation-promoting factor by activating the Arp2/3 complex to induce actin polymerization (PubMed:19633175). Participates in both linear- and branched-actin networks (PubMed:19633175). Functions in linear-filament (bundled F-actin) by acting downstream of Rho1 and regulating actin and microtubule organization during oogenesis (PubMed:19633175). Nucleates actin in an Arp2/3-dependent manner and exhibits F-actin and microtubule bundling and cross-linking activity in the egg chamber (PubMed:19633175). During embryogenesis, acts downstream of Rho1 to activate the Arp2/3 complex which is necessary for the developmental migration of tail hemocytes anteriorly along the ventral midline (PubMed:25739458). Its function in hemocyte transmigration is independent of the WASH complex (PubMed:25739458). May play a role in endosomal sorting; its assembly in the WASH complex may regulate its nucleation-promoting factor (NPF) activity.
(UniProt, Q7JW27)
Summary (Interactive Fly)

cytoskeletal protein - acts upstream of Arp2/3 which in turn mediates actin nucleation activity - functions downstream of Rho1 and the formin Cappuccino to control actin and microtubule dynamics during oogenesis - bundles and crosslinks F-actin and microtubules

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\wash for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q7JW27)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
wash-RA
FBtr0087989
NM_136895
1751
499
wash-RB
FBtr0339595
NM_001299405
1796
499
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
wash-PA
FBpp0087097
53.2
499
6.10
Q7JW27
NP_610739
AAF58562
wash-PB
FBpp0308667
53.2
499
6.10
Q7JW27
NP_001286334
AHN56132
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

499 aa isoforms: wash-PA, wash-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Component of the WASH complex (PubMed:20498093). Interacts with spir and capu (PubMed:19633175). Interacts (via N-terminus) with Rho1 (via N-terminus) (PubMed:25739458).

(UniProt, Q7JW27)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\wash using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

5.13

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
embryonic/larval hemocyte
(Verboon et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Verboon et al., 2015, Rodriguez-Mesa et al., 2012)
located_in endosome
inferred from direct assay
(Dong et al., 2013)
colocalizes_with nuclear envelope
inferred from direct assay
(Verboon et al., 2020)
located_in nuclear envelope
inferred from direct assay
(Verboon et al., 2015)
located_in nucleoplasm
inferred from direct assay
(Verboon et al., 2020)
located_in nucleus
inferred from direct assay
(Verboon et al., 2015, Rodriguez-Mesa et al., 2012)
located_in perinuclear region of cytoplasm
inferred from direct assay
(Rodriguez-Mesa et al., 2012)
part_of WASH complex
inferred from direct assay
(Jia et al., 2010)
inferred from direct assay
(Verboon et al., 2020)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\wash in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 3 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 20 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of wash
Transgenic constructs containing regulatory region of wash
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(2R)BSC699
(Verboon et al., 2018)
Inferred from location ( 0 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal - all die during embryonic stage
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
viable (with Df(2R)BSC699)
Sterility
Allele
female fertile
female semi-sterile
fertile
Other Phenotypes
Allele
abnormal cell migration, with Scer\GAL4Pxn.PS
abnormal cell migration | prepupal stage
abnormal immune response | prepupal stage
abnormal size | larval stage
abnormal wound healing | prepupal stage
enhancer of variegation, with In(1)wm4
short lived | adult stage | nutrition conditional
suppressor of variegation, with Dp(?;2)bwD
suppressor of variegation, with In(2R)bwVDe2
Phenotype manifest in
Allele
autolysosome | third instar larval stage | nutrition conditional
autophagosome | third instar larval stage
autophagosome | third instar larval stage | nutrition conditional
Cajal body | larval stage
cell projection, with Scer\GAL4Pxn.PS
chromosome | larval stage
cortical actin cytoskeleton | oogenesis
cortical actin cytoskeleton | oogenesis, with Scer\GAL4VP16.mat.αTub67C
cortical actin cytoskeleton | oogenesis (with Df(2R)BSC699)
egg chamber
egg chamber, with Scer\GAL4VP16.mat.αTub67C
egg chamber (with Df(2R)BSC699)
embryonic/larval hemocyte, with Scer\GAL4Pxn.PS
embryonic/larval plasmatocyte | prepupal stage
embryonic/larval plasmatocyte | pupal stage
embryonic/larval plasmatocyte | pupal stage, with Scer\GAL4Hml.Δ
embryonic/larval plasmatocyte | third instar larval stage
embryonic/larval salivary gland | larval stage
embryonic/larval tracheal dorsal trunk
fat body | third instar larval stage
fat body | third instar larval stage | nutrition conditional
female germline ring canal
female germline ring canal, with Scer\GAL4VP16.mat.αTub67C
female germline ring canal (with Df(2R)BSC699)
focal adhesion | pupal stage
lysosome | pupal stage
lysosome | third instar larval stage
lysosome | third instar larval stage | nutrition conditional
nuclear envelope | larval stage
nucleolus | larval stage
nucleus | larval stage
oocyte
oocyte, with Scer\GAL4VP16.mat.αTub67C
oocyte (with Df(2R)BSC699)
oocyte | oogenesis stage S7, with Scer\GAL4VP16.mat.αTub67C
salivary gland | larval stage
vacuole | increased number, with Scer\GAL4Pxn.PS
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (5)
Hsap\WASHC1
13 of 14
Yes
Yes
Hsap\WASH6P
11 of 14
No
Yes
Hsap\WASH2P
9 of 14
No
Yes
Hsap\WASH3P
9 of 14
No
Yes
Hsap\WASH4P
9 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (1)
Rnor\Washc1
13 of 14
Yes
Yes
Mus musculus (laboratory mouse) (1)
Mmus\Washc1
13 of 14
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\washc1
11 of 13
Yes
Yes
Danio rerio (Zebrafish) (1)
Drer\wash1
14 of 14
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\ddl-2
7 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP010756
12 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:wash. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    WASHC1; WASH complex subunit 1
    13 of 14
    613632
        WASH6P; WASP family homolog 6, pseudogene
        11 of 14
            WASH2P; WASP family homolog 2, pseudogene
            9 of 14
                WASH3P; WASP family homolog 3, pseudogene
                9 of 14
                    WASH4P; WASP family homolog 4, pseudogene
                    9 of 14
                        Functional Complementation Data
                        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                        Interactions
                        Summary of Physical Interactions
                        esyN Network Diagram
                        Show neighbor-neighbor interactions:
                        Show/hide secondary interactors 
                        (data from AllianceMine provided by esyN)
                        Select Layout:
                        Legend:
                        Protein
                        RNA
                        Selected Interactor(s)
                        Other Interaction Browsers
                        View in FlyBase Interactions Browser   View in MIST tool (external link)
                        Please see the Physical Interaction reports below for full details
                        protein-protein
                        Physical Interaction
                        Assay
                        References
                        wash
                        pull down, autoradiography
                        (Verboon et al., 2020, Liu et al., 2009)
                        wash - Btk
                        anti tag coimmunoprecipitation, anti tag western blot, western blot
                        (Tsarouhas et al., 2019)
                        wash - CCDC53
                        pull down, autoradiography, blue native page, western blot, tandem affinity purification, Identification by mass spectrometry, anti bait coimmunoprecipitation
                        (Davidson et al., 2023, Verboon et al., 2020, Jia et al., 2010)
                        wash - CG5446
                        two hybrid
                        (Hu et al., 2017.6.13)
                        wash - E(bx)
                        anti bait coimmunoprecipitation, western blot
                        (Kwon et al., 2016)
                        wash - FAM21
                        anti bait coimmunoprecipitation, western blot, comigration in non denaturing gel electrophoresis, blue native page, tandem affinity purification, Identification by mass spectrometry
                        (Davidson et al., 2023, Verboon et al., 2020, Jia et al., 2010)
                        wash - Lam
                        anti bait coimmunoprecipitation, western blot, pull down, autoradiography, blue native page, proximity ligation assay
                        (Verboon et al., 2020, Verboon et al., 2015)
                        wash - Rho1
                        pull down, western blot, autoradiography, anti bait coimmunoprecipitation, anti tag western blot
                        (Verboon et al., 2015, Liu et al., 2009)
                        wash - SWIP
                        tandem affinity purification, Identification by mass spectrometry
                        (Jia et al., 2010)
                        wash - Strump
                        blue native page, western blot, anti bait coimmunoprecipitation, tandem affinity purification, Identification by mass spectrometry
                        (Verboon et al., 2020, Jia et al., 2010)
                        wash - Vha55
                        anti tag coimmunoprecipitation, anti tag western blot
                        (Nagel et al., 2017)
                        wash - capu
                        anti bait coimmunoprecipitation, western blot
                        (Liu et al., 2009)
                        wash - cher
                        phenotype-based detection assay, fluorescence microscopy, inferred by author
                        (Green et al., 2018)
                        wash - pav
                        pull down, autoradiography, anti tag coimmunoprecipitation, Identification by mass spectrometry, comigration in non denaturing gel electrophoresis, western blot, anti bait coimmunoprecipitation
                        (Davidson et al., 2023)
                        wash - spir
                        pull down, autoradiography, anti bait coimmunoprecipitation, western blot
                        (Liu et al., 2009)
                        wash - tum
                        anti bait coimmunoprecipitation, western blot, pull down, autoradiography, comigration in non denaturing gel electrophoresis
                        (Davidson et al., 2023)
                        Summary of Genetic Interactions
                        esyN Network Diagram
                        Other Interaction Browsers
                        Starting gene(s)
                        Interaction type
                        Interacting gene(s)
                        Reference
                        Starting gene(s)
                        Interaction type
                        Interacting gene(s)
                        Reference
                        External Data
                        Subunit Structure (UniProtKB)
                        Component of the WASH complex (PubMed:20498093). Interacts with spir and capu (PubMed:19633175). Interacts (via N-terminus) with Rho1 (via N-terminus) (PubMed:25739458).
                        (UniProt, Q7JW27 )
                        Linkouts
                        BioGRID - A database of protein and genetic interactions.
                        DroID - A comprehensive database of gene and protein interactions.
                        MIST (protein-protein) - An integrated Molecular Interaction Database
                        Pathways
                        Signaling Pathways (FlyBase)
                        Metabolic Pathways
                        External Data
                        Linkouts
                        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                        Genomic Location and Detailed Mapping Data
                        Chromosome (arm)
                        2R
                        Recombination map
                        2-66
                        Cytogenetic map
                        48E6-48E6
                        Sequence location
                        FlyBase Computed Cytological Location
                        Cytogenetic map
                        Evidence for location
                        48E6-48E6
                        Limits computationally determined from genome sequence between P{PZ}SmD302833 and P{PZ}Cam03909
                        Experimentally Determined Cytological Location
                        Cytogenetic map
                        Notes
                        References
                        Experimentally Determined Recombination Data
                        Location

                        2-66

                        (FlyBase, 2016)

                        2-67.4

                        (Comeron et al., 2012)
                        Left of (cM)
                        Right of (cM)
                        Notes
                        Stocks and Reagents
                        Stocks (17)
                        Genomic Clones (15)
                         

                        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

                        cDNA Clones (65)
                         

                        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

                        cDNA clones, fully sequenced
                        BDGP DGC clones
                        Other clones
                          Drosophila Genomics Resource Center cDNA clones

                          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                          cDNA Clones, End Sequenced (ESTs)
                          BDGP DGC clones
                          Other clones
                          RNAi and Array Information
                          Linkouts
                          DRSC - Results frm RNAi screens
                          Antibody Information
                          Laboratory Generated Antibodies
                           

                          monoclonal

                          (Verboon et al., 2018, Verboon et al., 2015, Rodriguez-Mesa et al., 2012, Liu et al., 2009)
                          Commercially Available Antibodies
                           
                          Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
                          Cell Line Information
                          Publicly Available Cell Lines
                           
                            Other Stable Cell Lines
                             
                              Other Comments

                              Identification: as a protein found in a Trf2-containing complex.

                              (Hochheimer et al., 2002)
                              Relationship to Other Genes
                              Source for database merge of
                              Additional comments
                              Nomenclature History
                              Source for database identify of

                              Source for identity of: wash CG13176

                              (Linardopoulou et al., 2007)
                              Nomenclature comments
                              Etymology

                              The gene symbol "wash" is an acronym formed from "Wiskott-Aldrich Syndrome Protein and SCAR Homolog".

                              (Linardopoulou et al., 2007)
                              Synonyms and Secondary IDs (8)
                              Datasets (0)
                              Study focus (0)
                              Experimental Role
                              Project
                              Project Type
                              Title
                              Study result (0)
                              Result
                              Result Type
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                              External Crossreferences and Linkouts ( 44 )
                              Sequence Crossreferences
                              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                              UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                              UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                              Other crossreferences
                              AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                              BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                              DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                              EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                              FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                              FlyMine - An integrated database for Drosophila genomics
                              InterPro - A database of protein families, domains and functional sites
                              KEGG Genes - Molecular building blocks of life in the genomic space.
                              MARRVEL_MODEL - MARRVEL (model organism gene)
                              Linkouts
                              BioGRID - A database of protein and genetic interactions.
                              Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                              DroID - A comprehensive database of gene and protein interactions.
                              DRSC - Results frm RNAi screens
                              Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
                              Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                              FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                              FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                              Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                              Flygut - An atlas of the Drosophila adult midgut
                              iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                              Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                              KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                              MIST (protein-protein) - An integrated Molecular Interaction Database
                              Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                              References (63)