FlyBase Gene Report: Dmel\Ent1

General Information

Symbol

Dmel\Ent1

Species

D. melanogaster

Name

Equilibrative nucleoside transporter 1

Annotation Symbol

CG11907

Feature Type

protein_coding_gene

FlyBase ID

FBgn0031250

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

21C1-21C1

Sequence location

2L:355,565..357,874 [-]

Recombination map

2-0.7

RefSeq locus

NT_033779 REGION:355565..357874

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables nucleoside transmembrane transporter activity

inferred from biological aspect of ancestor with PANTHER:PTN000035696

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR002259

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with HGNC:11003

(Sankar et al., 2002)

inferred from sequence or structural similarity with HGNC:11004

(Sankar et al., 2002)

inferred from sequence or structural similarity with UniProtKB:Q9VMB6

(Gene Ontology Curators, 2002-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in nucleoside transmembrane transport

inferred from sequence or structural similarity with UniProtKB:Q9VMB6

(Gene Ontology Curators, 2002-)

inferred from electronic annotation with InterPro:IPR002259

(InterPro Project Members, 2004-)

involved_in nucleoside transport

inferred from sequence or structural similarity with HGNC:11003

(Sankar et al., 2002)

inferred from sequence or structural similarity with HGNC:11004

(Sankar et al., 2002)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in membrane

inferred from electronic annotation with InterPro:IPR002259

(InterPro Project Members, 2004-)

inferred from sequence model

(Sankar et al., 2002)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000035696

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

SLC29 FAMILY OF EQUILIBRATIVE NUCLEOSIDE TRANSPORTERS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

SLC29 FAMILY OF EQUILIBRATIVE NUCLEOSIDE TRANSPORTERS -: Solute carrier family 29 (SLC29) transporters transport nucleosides across membranes by facilitated diffusion. (Adapted from FBrf0152285).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ent1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VPP0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.44

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ent1-RA

FBtr0078094

NM_164381

1972

476

Ent1-RB

FBtr0078095

NM_134675

1923

476

Ent1-RC

FBtr0330651

NM_001272891

1905

476

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Ent1-PA

FBpp0077753

53.1

476

5.21

FBpp0077754

53.1

476

5.21

FBpp0303501

53.1

476

5.21

Q9VPP0

NP_001259820

AGB92357

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

476 aa isoforms: Ent1-PA, Ent1-PB, Ent1-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ent1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ent1_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.64

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | faint | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Ent1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ent1

View exonic expression by tissue for Dmel\Ent1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0031250

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33207/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0031250

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0031250

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0031250

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11907

Flygut - An atlas of the Drosophila adult midgut

FBgn0031250

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ent1

Transgenic constructs containing regulatory region of Ent1

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | pharate adult stage, with Scer\GAL4^Act5C.PI

Ent1^GD783

lethal | pupal stage, with Scer\GAL4^Act5C.PI

Ent1^GD783

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Ent1^GD783

viable, with Scer\GAL4^pnr-MD237

Ent1^GD783

viable, with Scer\GAL4^tin.CΔ4

Ent1^GD783

Other Phenotypes

Allele

visible, with Scer\GAL4^Bx-MS1096

Ent1^GD783

visible, with Scer\GAL4^pnr-MD237

Ent1^GD783

Phenotype manifest in

Allele

scutellar bristle, with Scer\GAL4^Act5C.PI

Ent1^GD783

scutellar bristle | ectopic, with Scer\GAL4^Act5C.PI

Ent1^GD783

trichogen cell, with Scer\GAL4^pnr-MD237

Ent1^GD783

wing, with Scer\GAL4^Bx-MS1096

Ent1^GD783

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (4)

Hsap\SLC29A1

11 of 14

Yes

Hsap\SLC29A3

11 of 14

Yes

Hsap\SLC29A2

10 of 14

Yes

Hsap\SLC29A4

3 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (4)

Rnor\Slc29a1

13 of 14

Yes

Rnor\Slc29a2

11 of 14

Yes

Rnor\Slc29a3

9 of 14

Yes

Rnor\Slc29a4

3 of 14

Mus musculus (laboratory mouse) (4)

Mmus\Slc29a1

12 of 14

Yes

Mmus\Slc29a2

9 of 14

Yes

Mmus\Slc29a3

9 of 14

Yes

Mmus\Slc29a4

3 of 14

Xenopus tropicalis (Western clawed frog) (3)

Xtro\slc29a1

7 of 13

Yes

Xtro\slc29a2

6 of 13

Yes

Xtro\slc29a3

1 of 13

Yes

Danio rerio (Zebrafish) (7)

Drer\slc29a1a

12 of 14

Yes

Drer\slc29a1b

12 of 14

Yes

Drer\slc29a2

11 of 14

Yes

Drer\slc29a3

9 of 14

Yes

Drer\slc29a4b

3 of 14

Drer\slc29a4a

2 of 14

Drer\LOC100334018

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\ent-6

8 of 14

Yes

7 of 14

7 of 14

6 of 14

6 of 14

6 of 14

6 of 14

Anopheles gambiae (African malaria mosquito) (4)

Agam\AgaP_AGAP009114

12 of 12

Yes

Agam\AgaP_AGAP003892

6 of 12

Agam\AgaP_AGAP011796

3 of 12

Agam\AgaP_AGAP012748

3 of 12

Arabidopsis thaliana (thale-cress) (8)

Atha\ENT1

9 of 13

Yes

Atha\ENT6

9 of 13

Yes

Atha\ENT7

9 of 13

Yes

Atha\ENT4

8 of 13

Yes

Atha\FUR1

8 of 13

Yes

Atha\AT1G02630

7 of 13

Atha\AT3G09990

7 of 13

Yes

Atha\AT4G05140

6 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\FUN26

13 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Ent1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (2)

Ent2

10 of 13

Ent3

4 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

histiocytosis-lymphadenopathy plus syndrome

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

SLC29A3; solute carrier family 29 member 3

model of histiocytosis-lymphadenopathy plus syndrome

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SLC29A1; solute carrier family 29 member 1 (Augustine blood group)

11 of 14

602193

SLC29A3; solute carrier family 29 member 3

11 of 14

612373

HISTIOCYTOSIS-LYMPHADENOPATHY PLUS SYNDROME

histiocytosis-lymphadenopathy plus syndrome

SLC29A2; solute carrier family 29 member 2

10 of 14

602110

SLC29A4; solute carrier family 29 member 4

3 of 14

609149

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

MIST Molecular Interaction Search Tool

View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Ent1 - CG14717

experimental knowledge based

(Guruharsha et al., 2011)

Ent1 - CG4325

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

59463

DroID - A comprehensive database of gene and protein interactions.

FBgn0031250

MIST (protein-protein) - An integrated Molecular Interaction Database

33207

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-0.7

Cytogenetic map

21C1-21C1

Sequence location

2L:355,565..357,874 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

21C1-21C1

Limits computationally determined from genome sequence between P{lacW}U2af38^k14504 and P{lacW}RpI135^k16513&P{lacW}ebi^k16213

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-0.7

(FlyBase, 2016)

2-0.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

82733

y¹ v¹; P{TKO.GS05076}attP40

51055

y¹ v¹; P{TRiP.HMJ21190}attP40

78945

y¹ w^*; TI{CRIMIC.TG4.0}Ent1^{CR00546-TG4.0}

17019

w¹¹¹⁸; P{EP}Ent1^EP2066 asRNA:CR45366^EP2066

NIG-Fly

M2L-2904

y¹ w¹¹¹⁸; Ent1^SK1 P{neoFRT}40A/CyO-Tb

M2L-2905

y¹ w¹¹¹⁸; Ent1^SK2 P{neoFRT}40A/CyO-Tb

2LG-2290

y² VhaAC39-1^cho-1 v¹; P{U6-Ent1.NIG.sgRNA}attP40/CyO

VDRC

v109885

P{KK101972}VIE-260B

v49328

w¹¹¹⁸ P{GD783}v49328

Genomic Clones (10)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (38)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD19162

Other clones

DPiM_LD19162

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0031250

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0031250

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: CG11907 anon- EST:fe2G2

(FlyBase, 1996-)

Additional comments

Source for merge of CG11907 anon- EST:fe2G2 was sequence comparison ( date:000605 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Ent1 CG11907

(Sankar et al., 2002)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

CG11907

(National Institute of Genetics Fly Stocks, 2022-, Fleischmannova et al., 2012, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Molnar et al., 2006, Bianchi-Frias et al., 2004, Giot et al., 2003, Sankar et al., 2002)

DmENT1

(Sankar et al., 2002)

DmEnt1

(Fleischmannova et al., 2012)

ENT1

(Machado et al., 2007)

Ent1

(Ceder and Fredriksson, 2022, Gene Disruption Project members, 2018-, Velentzas et al., 2018, Transgenic RNAi Project members, 2017-, Korenjak et al., 2014, Japanese National Institute of Genetics, 2012.5.21, Featherstone, 2011, Molnar et al., 2006)

anon-EST:fe2G2

dENT1

(Cliffe et al., 2009)

ent1

(Klaus et al., 2023, Knight et al., 2010)

Name Synonyms

Equilibrative nucleoside transporter 1

(Sankar et al., 2002)

anon-fast-evolving-2G2

Secondary FlyBase IDs

FBgn0025211

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 45 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33207

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VPP0

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VPP0

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0031250

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33207/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0031250

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0031250

FlyMine - An integrated database for Drosophila genomics

FBgn0031250

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG11907

MARRVEL_MODEL - MARRVEL (model organism gene)

33207

Linkouts

BioGRID - A database of protein and genetic interactions.

59463

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0031250

DroID - A comprehensive database of gene and protein interactions.

FBgn0031250

DRSC - Results frm RNAi screens

FBgn0031250

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ent1_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0031250

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0031250

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11907

Flygut - An atlas of the Drosophila adult midgut

FBgn0031250

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0031250

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC008473

MIST (protein-protein) - An integrated Molecular Interaction Database

33207

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (47)

The following external sites may use different assemblies or annotations than FlyBase.

-0.64

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