secreted growth factor - Tsg dislodges latent BMPs bound to Short gastrulation thus activating BMP signaling - makes a tripartite complex with Sog and a Dpp/Scw heterodimer - a favoured substrate for Tolloid, which processes Sog and liberates the Dpp/Scw heterodimer
Please see the JBrowse view of Dmel\tsg for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.39
Gene model reviewed during 5.41
Gene model reviewed during 5.45
1.0 (northern blot)
There is only one protein coding transcript and one polypeptide associated with this gene
249 (aa)
249 (aa); 27 (kD predicted)
Component of a complex composed of dpp, sog and tsg.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\tsg using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: reference states 1.5-3 hr AEL
tsg transcripts are expressed only in early embryos.
tsg transcripts are detected at 1.5-3hr of embryonic development and not at any other time during development. They first appear at 1hr and 45min of development in two domains, a broad dorsal saddle and an anterior cap. During cellularization, expression disappears from the dorsal midline and refines into a series of 4 diffuse stripes along the A/P axis. As the germband extends during stages 7 and 8, anterior expression fades and the mid dorsal stripes are located between the anterior transverse furrow (ATF) and the posterior transverse furrow (PTF). As the PTF deepens, the tsg-expressing cells become incorporated into the PTF. Double staining with ftz shows that the domain of tsg expression lies between parasegments 4 and 10.
JBrowse - Visual display of RNA-Seq signals
View Dmel\tsg in JBrowse1-38
1-37.1
1-36.79
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
The expression pattern of tsg from 5 Drosophila species is studied. Evidence for recent changes in the expression pattern between sibling species diverged only 2-5 million years ago is found and examples of changes that have been fixed to certain lineages 60 million years ago.
tsg protein is secreted and can act over a long range.
The restricted effect of tsg mutants, in contrast to those of dpp, tld and scw, and the structural features of a secreted protein suggest an alternative to the single morphogen (dpp) gradient model. Namely that tsg represents a second class of peptide growth factor that may be part of a combinatorial signalling mechanism that specifies dorsal patterning.
Zygotically active locus involved in the terminal developmental program in the embryo.
Involved in the regulatory hierarchy responsible for the asymmetric distribution and function of zygotic regulatory gene products along the DV axis of early embryos. tsg is required for the refinement of the zen expression pattern during cellularization and gastrulation: zen products do not become restricted to the presumptive amnioserosa.
Mutants are embryonic lethals and show abnormal gastrulation, with deep dorsal folds resulting in temporary blockage of germband extension. Later, dorsal folds released, but posterior midgut abnormal in position, dorsal cells very thick and cephalic folds very deep. At the end of embryonic development, tsg cuticle shows head defects and condensed, retracted posterior spiracles. Ventral nervous system split posteriorly. No particular cell must be wild type for survival in tsg/+ mosaics, but wild-type cells on dorsal side are most effective in rescue.
tsg mutants display abnormal gastrulation, head and posterior spiracles are defective in larval cuticle, ventral nervous system is split anteriorly.
Source for merge of: tsg l(1)11Ac
Source for identity of: tsg CG1502
