p62, Ref2P, SQSTM1, ref, Ref(2)P/p62
adaptor protein for delivering cargo marked by polyubiquitin to autophagosomes - homolog of the sequestosome marker SQSTM1/p62 - together with Dachs, continuously downregulated by autophagy in enterocytes, ensuring gut homeostasis in the non-infected state - a component of the lysosomal-autophagic compartment - a chaperone that regulates tau solubility thereby preventing tau aggregation
Please see the JBrowse view of Dmel\ref(2)P for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.52
2.4, 2.3 (unknown)
The group(s) of polypeptides indicated below share identical sequence to each other.
115-125 (kD observed); 76 (kD predicted)
Interacts with aPKC and Traf6.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ref(2)P using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: maternally deposited
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
JBrowse - Visual display of RNA-Seq signals
View Dmel\ref(2)P in JBrowse
2-54
2-53.7
2-54.0
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
ref(2)P is required for the normal clearance of the paternal mitochondrial derivatives in developing embryos.
The N-terminal domain of the ref(2)P protein is required for its activity in vivo. Conformation-dependent epitopes common to those of the Sigma map to a site distinct from the site involved in virus restriction. Two distinct domains of the ref(2)P protein are involved in the control of Sigma multiplication.
The ref(2)P locus shows unusual variability at the molecular level.
Invasion hypothesis that Type II virus (resistant to ref(2)Pp genotype) is spreading by vertical transmission from central France through European populations is confirmed by data from Tubingen where Type II clones increased from 0.27 to 0.93 between 1985 and 1991. However over the same period no increase in the frequency of infected flies was observed.
Development of the P-M system of transposable elements shows interesting correlations with the distribution of sigma rhabdovirus in D.melanogaster populations.
ref(2)P thought to act at level of sigma virus replication.
Males homozygous for null alleles sterile; elongating spermatids display degeneration of axonemes.
Strains of sigma virus capable of multiplication in restrictive strains can be selected; P+ virus strains multiply in ref(2)Pp hosts, whereas P- strains do not; no such distinction in ref(2)Po hosts. Also 5-FU-induced host-adapted strains of virus (haP) capable of replication in ref(2)Pp; many are temperature-sensitive, suggesting that ref(2)Pp interacts with a viral protein.
ref(2)Pp is the first refractaire mutant found and the most thoroughly studied. Allele frequency about 0.2 in Paris population and approaches the same incidence in caged populations with or without the presence of sigma virus.
Mutant that interferes with the normal multiplication of some strains of sigma virus, as indicated by delayed acquisition of CO2 sensitivity following viral injection.
permissive and restrictive alleles are codominant.
