FlyBase Allele Report: Dmel\ref(2)P[od2]

General Information

Symbol

Dmel\ref(2)P^od2

Species

D. melanogaster

Name

FlyBase ID

FBal0032567

Feature type

allele

Associated gene

Dmel\ref(2)P

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

diepoxybutane

Nature of the Allele

Allele class

loss of function allele

(Gay and Contamine, 1993)

Mutagen

diepoxybutane

(Gay and Contamine, 1993)

Progenitor genotype

ref(2)P^o

(Gay and Contamine, 1993)

Cytology

Description

31bp deletion associated with an imperfect 12bp duplication at one end of the deletion in the coding region in exon 1. This results in a stop codon in place of amino acid residue 9.

(Gay and Contamine, 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage | progressive

(de Castro et al., 2013)

abnormal stress response

(de Castro et al., 2013)

male sterile

(Bichon et al., 2001, Gay and Contamine, 1993)

short lived

(de Castro et al., 2013)

Phenotype Manifest In

indirect flight muscle cell

(de Castro et al., 2013, Pimenta de Castro et al., 2012)

mitochondrial derivative

(de Castro et al., 2013)

mitochondrial derivative (with ref(2)P^od3)

(Politi et al., 2014)

mitochondrion

(de Castro et al., 2013, Pimenta de Castro et al., 2012)

Nebenkern

(de Castro et al., 2013)

Detailed Description

Statement

Reference

ref(2)P^od2/ref(2)P^od3 mutant midgut cells at 2hr after puparium formation show similar amount of mitochondria as in controls.

(Anding et al., 2018)

Embryos derived from ref(2)P^od2/ref(2)P^od3 females show a moderate delay in the clearance of the paternal mitochondrial derivatives compared to wild type.

(Politi et al., 2014)

Early onion-stage spermatids of mutant males have abnormal nebenkern (mitochondrial derivatives) which envelop the white nuclei instead of being adjacent to them. The mutant spermatids undergo normal elongation, but individualisation is abnormal. There is an increased distance between the axoneme and mitochondrial derivative than normal in post-individualisation cysts, and the mitochondrial derivative contains a large mass of electron-dense material.

The density of mitochondrial DNA nucleoids in the indirect flight muscle is increased in mutant flies compared to wild type.

Mutants show an increase in mitochondrial DNA heteroplasmy compared to wild type in both 3 day old and 25 day old adults.

Mutant animals show a decrease in lifespan compared to wild type, both on normal food, or on food supplemented with either rotenone or paraquat. Locomotor activity is impaired from a young age and progresses with age.

(de Castro et al., 2013)

Mutants show mitochondrial defects in the indirect flight muscles.

(Pimenta de Castro et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ref(2)P^od2 has male sterile phenotype, suppressible by mPges2^GlSbH/Su(P)[+]

(Bichon et al., 2001)

ref(2)P^od2 has male sterile phenotype, suppressible by Su(P)[+]/mPges2^TW2

(Bichon et al., 2001)

ref(2)P^od2 has male sterile phenotype, suppressible by Su(P)[+]/mPges2^db

(Bichon et al., 2001)

ref(2)P^od2 has male sterile phenotype, suppressible by mPges2^hd48/Su(P)[+]

(Bichon et al., 2001)

ref(2)P^od2 has male sterile phenotype, suppressible by mPges2^hd5/Su(P)[+]

(Bichon et al., 2001)

ref(2)P^od2 has male sterile phenotype, suppressible by mPges2^pe/Su(P)[+]

(Bichon et al., 2001)

ref(2)P^od2 has male sterile phenotype, suppressible by mPges2^pr26/Su(P)[+]

(Bichon et al., 2001)

ref(2)P^od2 has male sterile phenotype, suppressible by Su(P)[+]/mPges2^TW9

(Bichon et al., 2001)

NOT Suppressor of

Statement

Reference

ref(2)P^od2 is a non-suppressor of melanotic mass phenotype | recessive | third instar larval stage phenotype of Atg6¹

(Shravage et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

ref(2)P^od2 is a suppressor of mitochondrion phenotype of Pink1^B9

(de Castro et al., 2013)

ref(2)P^od2 is a suppressor of indirect flight muscle cell phenotype of Pink1^B9

(de Castro et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

ref(2)P^od2 fails to suppress the melanotic mass phenotype seen in Atg6¹ mutant third instar larvae.

(Shravage et al., 2013)

The mitochondrial clustering seen in the indirect flight muscles of Pink1^B9 flies is significantly suppressed by ref(2)P^od2.

Expression of park^Scer\UAS.cGa under the control of Scer\GAL4^da.G32 significantly suppresses the penetrance of the indented thorax phenotype seen in Pink1^B9 mutants. This suppression is reduced if the flies are also carrying ref(2)P^od2.

(de Castro et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

99019

w^*; ref(2)P^od2/CyO, P{ActGFP}JMR1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

ODP2

(Gay and Contamine, 1993)

Ref(2)P^od2

(Baumgartner et al., 2021, Ikeda et al., 2019)

Ref(2)p^OD2

(Anding et al., 2018)

p62^od2

(Politi et al., 2014)

ref(2)P^OD2

(Nagai et al., 2021, Shravage et al., 2013)

ref(2)P^od2

(de Castro et al., 2013, Pimenta de Castro et al., 2012, Nezis et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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