FlyBase Gene Report: Dmel\Pfk

General Information

Symbol

Dmel\Pfk

Species

D. melanogaster

Name

Phosphofructokinase

Annotation Symbol

CG4001

Feature Type

protein_coding_gene

FlyBase ID

FBgn0003071

Gene Model Status

Current

Stock Availability

13 publicly available

Enzyme Name (EC)

6-phosphofructokinase (2.7.1.11)

Gene Summary

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. (UniProt, P52034)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

l(2)06339, 6-phosphofructokinase

Key Links

Genomic Location

Cytogenetic map

46E8-46F1

Sequence location

2R:10,109,740..10,117,457 [+]

Recombination map

2-62

RefSeq locus

NT_033778 REGION:10109740..10117457

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables 6-phosphofructokinase activity

inferred from mutant phenotype

(Currie and Sullivan, 1994)

inferred from direct assay

(Munneke and Collier, 1985)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

enables 6-phosphofructokinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000344216

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR000023, InterPro:IPR009161, InterPro:IPR015912, InterPro:IPR022953, InterPro:IPR035966

(InterPro Project Members, 2004-)

enables AMP binding

inferred from biological aspect of ancestor with PANTHER:PTN000344216

(GO Reference Genome Project, 2011-)

enables ATP binding

inferred from electronic annotation with InterPro:IPR009161

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000344216

(GO Reference Genome Project, 2011-)

enables fructose-6-phosphate binding

inferred from biological aspect of ancestor with PANTHER:PTN000344216

(GO Reference Genome Project, 2011-)

enables identical protein binding

inferred from biological aspect of ancestor with PANTHER:PTN000344216

(GO Reference Genome Project, 2011-)

enables monosaccharide binding

inferred from biological aspect of ancestor with PANTHER:PTN000344216

(GO Reference Genome Project, 2011-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in fructose 1,6-bisphosphate metabolic process

inferred from direct assay

(Munneke and Collier, 1985)

involved_in fructose 6-phosphate metabolic process

inferred from direct assay

(Munneke and Collier, 1985)

involved_in glucose homeostasis

inferred from mutant phenotype

(Ugrankar et al., 2015)

involved_in glycolytic process

inferred from mutant phenotype

(Currie and Sullivan, 1994)

involved_in response to sucrose

inferred from mutant phenotype

(Garrido et al., 2015)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in canonical glycolysis

inferred from biological aspect of ancestor with PANTHER:PTN000344216

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:P47857

(Gene Ontology Curators, 2002-)

involved_in fructose 1,6-bisphosphate metabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000344216

(GO Reference Genome Project, 2011-)

involved_in fructose 6-phosphate metabolic process

inferred from electronic annotation with InterPro:IPR022953

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000344216

(GO Reference Genome Project, 2011-)

involved_in glycolytic process

inferred from electronic annotation with InterPro:IPR000023, InterPro:IPR009161, InterPro:IPR015912, InterPro:IPR022953, InterPro:IPR035966

(InterPro Project Members, 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

part_of 6-phosphofructokinase complex

inferred by curator from GO:0003872

(Currie and Sullivan, 1994)

inferred from biological aspect of ancestor with PANTHER:PTN000344216

(GO Reference Genome Project, 2011-)

located_in cytoplasm

inferred from electronic annotation with InterPro:IPR009161

(InterPro Project Members, 2004-)

located_in cytosol

inferred from sequence or structural similarity with UniProtKB:P47857

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

CARBOHYDRATE KINASES

Protein Family (UniProt)

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily. (P52034)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

6-phosphofructokinase activity

ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6- bisphosphate + H(+) (2.7.1.11)

RHEA 16109:

Summaries

Gene Group (FlyBase)

CARBOHYDRATE KINASES -: Carbohydrate kinases catalyze the transfer of a phosphate group, usually from ATP, to a carbohydrate substrate molecule.

Protein Function (UniProtKB)

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

(UniProt, P52034)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Pfk for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P52034)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Pfk-RA

FBtr0088420

NM_078952

3217

950

Pfk-RB

FBtr0088422

NM_165746

3508

788

Pfk-RC

FBtr0088421

NM_165747

3188

788

Pfk-RD

FBtr0304925

NM_001259305

3217

950

Pfk-RE

FBtr0333067

NM_001273918

3133

922

Additional Transcript Data and Comments

Reported size (kB)

3.25, 3.0, 2.85 (northern blot)

(Currie and Sullivan, 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Pfk-PA

FBpp0087506

105.2

950

8.65

NP_523676

AAF58840

Pfk-PB

FBpp0087508

86.6

788

6.81

FBpp0087507

86.7

788

6.74

FBpp0293464

105.2

950

8.66

FBpp0305281

102.5

922

8.58

A0A0B4KFI7

NP_001260847

AGB93380

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

787 (aa)

(Currie and Sullivan, 1994)

Comments

External Data

Subunit Structure (UniProtKB)

Homotetramer.

(UniProt, P52034)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pfk using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pfk_1
Pfk_2

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

2.26

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 13 -- 16

dorsal pharyngeal muscle cell

(Fisher et al., 2012)

embryonic/larval muscle system

(Fisher et al., 2012)

embryonic/larval somatic muscle cell

(Fisher et al., 2012)

embryonic stage 15

somatic muscle cell

(Tixier et al., 2013)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Currie and Sullivan, 1994)

Additional Descriptive Data

Pfk transcripts are detected at a high level in early embryos, decline at mid embryogenesis, and increase to a plateau during larval stages. They decrease in abundance during pupation and increase just prior to emergence. mRNA levels rapidly decline after emergence even though Pfk enzyme activity remains high in adults.

(Currie and Sullivan, 1994)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

enzyme assay or biochemical detection

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage -- adult stage

(Munneke and Collier, 1985)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 15

muscle cell

(Tixier et al., 2013)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010, Dorus et al., 2006)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Pfk specific activity was found to be very low during larval and early pupal stages. Activity plateaus at 24 hours posteclosion.

(Munneke and Collier, 1985)

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Pfk in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Pfk

View exonic expression by tissue for Dmel\Pfk

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003071

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36060/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003071

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003071

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003071

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4001

Flygut - An atlas of the Drosophila adult midgut

FBgn0003071

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Pfk

Stages(s) 1-3

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Pfk

Transgenic constructs containing regulatory region of Pfk

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(2R)X1

(Goldstein et al., 2001, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Gene not disrupted in

Df(2R)12

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2R)en-A

(BDGP Project Members, 1994-1999)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Act5C.PU

Pfk^GD1508

lethal (with Df(2R)X1)

Pfk⁰⁶³³⁹

lethal | recessive

Pfk⁰⁶³³⁹

lethal - all die before end of larval stage, with Scer\GAL4^da.PU

Pfk^GD1508

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Pfk^GD1508

viable, with Scer\GAL4^Mef2.PR

Pfk^GD1508

viable, with Scer\GAL4^pnr-MD237

Pfk^GD1508

viable, with Scer\GAL4^tin.CΔ4

Pfk^GD1508

Other Phenotypes

Allele

abnormal developmental rate | nutrition conditional, with Scer\GAL4^Cg.PA

Pfk^GD1508

decreased efficacy of long-term memory | adult stage, with Scer\GAL4^GMR54H02

decreased efficacy of medium-term memory | adult stage, with Scer\GAL4^GMR54H02

Pfk^KK101887

decreased size | adult stage, with Scer\GAL4^C-765

Pfk^GD1508

flightless, with Scer\GAL4^Mef2.PR

Pfk^GD1508

short lived, with Scer\GAL4^da.PU

Pfk^GD1508

Phenotype manifest in

Allele

embryonic/larval somatic muscle cell

Pfk⁰⁶³³⁹

embryonic/larval somatic muscle cell, with Scer\GAL4^kirre.PU

Pfk^KK101887

embryonic myoblast

Pfk⁰⁶³³⁹

embryonic myoblast, with Scer\GAL4^kirre.PU

Pfk^KK101887

wing, with Scer\GAL4^C-765

Pfk^GD1508

wing disc dorsal compartment, with Scer\GAL4^ap-md544

Pfk^GD1508

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (3)

Hsap\PFKM

14 of 14

Yes

Hsap\PFKL

13 of 14

Yes

Hsap\PFKP

13 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (3)

Rnor\Pfkl

13 of 14

Yes

Rnor\Pfkm

13 of 14

Yes

Rnor\Pfkp

13 of 14

Yes

Mus musculus (laboratory mouse) (3)

Mmus\Pfkm

14 of 14

Yes

Mmus\Pfkp

13 of 14

Yes

Mmus\Pfkl

12 of 14

Yes

Xenopus tropicalis (Western clawed frog) (4)

Xtro\pfkm

8 of 13

Yes

Xtro\pfkp

7 of 13

Yes

Xtro\cmss1

1 of 13

Yes

Xtro\slc35f4

1 of 13

Danio rerio (Zebrafish) (6)

Drer\pfkma

14 of 14

Yes

Drer\pfkla

13 of 14

Yes

Drer\pfkmb

13 of 14

Yes

Drer\pfkpa

13 of 14

Yes

Drer\pfklb

12 of 14

Yes

Drer\pfkpb

12 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\pfk-1.1

13 of 14

Yes

Cele\pfk-1.2

7 of 14

Yes

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP007642

12 of 12

Yes

Arabidopsis thaliana (thale-cress) (12)

Atha\PFK7

7 of 13

Yes

Atha\PFK1

6 of 13

Yes

Atha\PFK3

6 of 13

Yes

Atha\PFK4

6 of 13

Yes

Atha\PFK5

6 of 13

Yes

Atha\PFK6

6 of 13

Yes

Atha\PFK2

5 of 13

Yes

Atha\AT1G12000

2 of 13

Yes

Atha\AT1G20950

2 of 13

Yes

Atha\AT1G76550

2 of 13

Yes

Atha\MEE51

2 of 13

Yes

Atha\AT4G08876

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\PFK2

12 of 13

Yes

Scer\PFK1

11 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\pfk1

11 of 12

Yes

Escherichia coli (enterobacterium) (1)

Ecol\pfkA

8 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Pfk. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Pfk^GD1508

model of disease of metabolism

CEA

(Devilliers et al., 2021)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

PFKM; phosphofructokinase, muscle

model of glycogen storage disease VII

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Pfk^HMS01324

ameliorates carcinoma

modeled by yki^{UAS.cOa.Tag:V5}, l(2)gl⁴

(Enzo et al., 2015)

exacerbates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^{G298S.UAS.YFP}

(Manzo et al., 2019)

modeled by Hsap\TARDBP^UAS.YFP.cEa

(Manzo et al., 2019)

ameliorates combined D-2- and L-2-hydroxyglutaric aciduria

modeled by sea^Δ24

(Li et al., 2018)

ameliorates congenital disorder of glycosylation Ij

modeled by Alg7^HMC03443

(Dalton et al., 2022)

Pfk^UAS.cTa

ameliorates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Besson et al., 2015)

DOES NOT ameliorate Leigh disease

modeled by ND-23^KK102085

(Cabirol-Pol et al., 2018)

ameliorates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^{G298S.UAS.YFP}

(Manzo et al., 2019)

modeled by Hsap\TARDBP^UAS.YFP.cEa

(Manzo et al., 2019)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PFKM; phosphofructokinase, muscle

14 of 14

610681

GLYCOGEN STORAGE DISEASE VII; GSD7

glycogen storage disease VII

PFKL; phosphofructokinase, liver type

13 of 14

171860

PFKP; phosphofructokinase, platelet

13 of 14

171840

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

l(2)gl|yki

suppressible

Pfk

(Enzo et al., 2015)

suppressible

(Li et al., 2020)

suppressible

(Besson et al., 2015)

Pdha

suppressible

Pfk

(Besson et al., 2015)

suppressible

(Li et al., 2020)

suppressible

(Devilliers et al., 2021)

trc

enhanceable

Pfk

(Hudson et al., 2023)

External Data

Subunit Structure (UniProtKB)

Homotetramer.

(UniProt, P52034 )

Linkouts

BioGRID - A database of protein and genetic interactions.

61888

DroID - A comprehensive database of gene and protein interactions.

FBgn0003071

MIST (protein-protein) - An integrated Molecular Interaction Database

36060

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Glycolysis

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-62

Cytogenetic map

46E8-46F1

Sequence location

2R:10,109,740..10,117,457 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

46E8-46F1

Limits computationally determined from genome sequence between P{PZ}14-3-3ζ⁰⁷¹⁰³ and P{PZ}Pfk⁰⁶³³⁹&P{PZ}CCS⁰³²²¹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

46F1-46F2

(determined by in situ hybridisation)

(Spradling et al., 1999)

46D-46E

(determined by in situ hybridisation)

(Currie and Sullivan, 1994)

46F1-46F2

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-62

(FlyBase, 2016)

2-60.3

(Comeron et al., 2012)

2-85.2

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

60675

w¹¹¹⁸; P{UAS-Pfk.T}3

12031

cn¹ P{PZ}Pfk⁰⁶³³⁹/CyO; ry⁵⁰⁶

78873

y¹ w^*; TI{CRIMIC.GT14}Pfk^CR00064

22605

y¹ w^67c23; P{EPgy2}Pfk^EY23126/CyO

36782

y¹ sc^* v¹ sev²¹; P{TRiP.GL00298}attP2

85757

y¹ sc^* v¹ sev²¹; P{TOE.GS01578}attP40

34336

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01324}attP2

77331

y¹ sc^* v¹ sev²¹; P{TKO.GS00945}attP40/CyO

NIG-Fly

HMS01324

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01324}attP2

VDRC

v105666

P{KK101887}VIE-260B

v3016

w¹¹¹⁸; P{GD1508}v3016

v3017

w¹¹¹⁸; P{GD1508}v3017

More stocks available...

Genomic Clones (26)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (118)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI18643

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0003071

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0003071

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Pfk is a single copy gene with a coding region that spans 6.5kb, composed of 8 exons and 7 intron. Alternate polyadenylation sites generate 3 developmentally regulated transcripts. Transcription initiation occurs at a single site.

(Currie and Sullivan, 1994)

Produces the glycolytic enzyme phosphofructokinase (PFK). Only one electrophoretic form has been found in larvae and adults.

Relationship to Other Genes

Source for database merge of

Source for merge of: Pfk l(2)06339

(Spradling et al., 1999)

Source for merge of: Pfk BcDNA:GH12192

(FlyBase, 1996-)

Additional comments

FlyBase curator comment: The "l(2)46Eb" complementation group is stated to correspond to Pfk in FBrf0145131, since members of this complementation group fail to complement the Pfk⁰⁶³³⁹ insertion allele (the insertion in the Pfk⁰⁶³³⁹ allele is within Pfk and multiple precise excisions can be obtained from this line, suggesting that the P-element insertion is responsible for the lethality of the line). However, this contradicts data from FBrf0076706 which suggests instead that the "l(2)46Fb" complementation group corresponds to Pfk. "l(2)46Fb", "l(2)46Eb" and Pfk have been kept as 3 separate genes in FlyBase until this discrepancy is resolved.

(Goldstein et al., 2001)

Source for merge of Pfk BcDNA:GH12192 was sequence comparison ( date:990717 ).

(FlyBase, 1996-)

FlyBase curator comment: The "l(2)46Fb" complementation group is stated to correspond to Pfk in FBrf0076706, since animals which are heterozygous for "l(2)46Fb" mutations show an approximately 50% reduction in Pfk activity compared to wild-type controls. However, this contradicts data from FBrf0145131, which suggests instead that the "l(2)46Eb" complementation group corresponds to Pfk. "l(2)46Fb", "l(2)46Eb" and Pfk have been kept as 3 separate genes in FlyBase until this discrepancy is resolved.

(Currie and Sullivan, 1994)

Nomenclature History

Source for database identify of

Source for identity of: Pfk CG4001

(Shue, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

BcDNA.GH12192

(Rubin, 1999.4.23)

BcDNA:GH12192

CG4001

(National Institute of Genetics Fly Stocks, 2022-, Homem et al., 2014, Chambers et al., 2012, Cammarato et al., 2011, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Derré et al., 2007, Pile et al., 2003)

PFK

(Rabah et al., 2023, de Tredern et al., 2021, Liguori et al., 2021, Manzo et al., 2019, Cabirol-Pol et al., 2018, Besson et al., 2015, Currie and Sullivan, 1994)

PFK1

(Garrido et al., 2015)

Pfk

(Bamgbose and Tulin, 2024, Fisher et al., 2023, Hudson et al., 2023, Livelo et al., 2023, Dalton et al., 2022, Ding et al., 2022, Hunt and Demontis, 2022, Zhu et al., 2022, Dar et al., 2021, Oka et al., 2021, Solana-Manrique et al., 2021, Beebe et al., 2020, Carvalho-Santos et al., 2020, Li et al., 2020, Solana-Manrique et al., 2020, van den Ameele and Brand, 2019, Wong et al., 2019, Bost et al., 2018, Crofton et al., 2018, Gene Disruption Project members, 2018-, Ugrankar et al., 2018, Valsecchi et al., 2018, Song et al., 2017, Transgenic RNAi Project members, 2017-, David-Morrison et al., 2016, Kuleesha et al., 2016, Aradska et al., 2015, Cogni et al., 2015, Enzo et al., 2015, Kwon et al., 2015, Ugrankar et al., 2015, Van Bortle et al., 2015, Zirin et al., 2015, Chen et al., 2014, de la Cova et al., 2014, Homem et al., 2014, Lavington et al., 2014, Whitaker et al., 2014, Yasunaga et al., 2014, Nirala et al., 2013, Tixier et al., 2013, Tixier et al., 2013, Chambers et al., 2012, Friedman et al., 2011, Tennessen et al., 2011, Gan et al., 2010, Wasbrough et al., 2010, Derré et al., 2007, Dietzl et al., 2007, Behm-Ansmant et al., 2006, Dorus et al., 2006, Rehwinkel et al., 2006)

l(2)06339

(Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

pfk

(Pang et al., 2023, Martínez-Morentin et al., 2015, Currie, 1994)

Name Synonyms

6-phosphofructokinase

(Sankaranarayanan and Weil, 2020, Li and White, 2003)

Phosphofructokinase

(Rice et al., 2020, de la Cova et al., 2014, Cammarato et al., 2011, Tennessen et al., 2011, Barnes et al., 2010)

Phosphofructokinase 1

(Garrido et al., 2015)

Phosphofructosekinase

(Krejčová et al., 2024)

phosphofructokinase

(Besson et al., 2015)

Secondary FlyBase IDs

FBgn0010618

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 83 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36060

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P52034

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P52034

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P52034

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003071

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36060/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003071

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003071

FlyMine - An integrated database for Drosophila genomics

FBgn0003071

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG4001

MARRVEL_MODEL - MARRVEL (model organism gene)

36060

Linkouts

BioGRID - A database of protein and genetic interactions.

61888

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0003071

DroID - A comprehensive database of gene and protein interactions.

FBgn0003071

DRSC - Results frm RNAi screens

FBgn0003071

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pfk_1
Pfk_2

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003071

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0003071

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4001

Flygut - An atlas of the Drosophila adult midgut

FBgn0003071

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0003071

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC005855

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

36060

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Glycolysis

References (136)

The following external sites may use different assemblies or annotations than FlyBase.

2.26

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