CAACAACAACCACAACTCGGTGCCGTTAGGGCTGCGTTTGTTTTCTGCTCCCATTCAGCTGCATAGAAAAGAAAGAAAAG TGAAAAGTGTTCGGAAAACAGGCAGCATGTGAAGCATAATTACTCCACGGAATTACTATCTCAAACGAAATAGTTGTCGC CGAAAAGAAGTGCGTAAAATCGCGAGTGAGCTCGTGAAAATAGAATAAAAAAGCGCTCTGCAATTGAAGAGAAACATTGC TGCAGTCGTGTGTACGTGCGTGTGTTTGTGCGTGCGAGTGTGAGTGTCGCTCTCTGGACGCAATTTCTTTGGCCAAAAGA GTGGGGAAGTGGGGCAGGAGAGTCCCGAATCGAATCCCCGAAACAGGCAATCCGAATCCGAATCCGGTGCATTAAAAATA GCGCAACGAGATGGTGAAGGCGCTGCTAACGAAGATGATAATGTAAATGCCGCATCGACGCATCCCCTTTCTCTTCTCTC TCCAAACGAAAGCGAAATCCCAGCTGGGAAACTGTAGCTCCAGCTAAAACCGAACCAGCAAAACAAAACCGAAATCGAAA CTGCATTCTTTGGACTCATATTTATATCACATAACGACACATATCGCCGACGGAACCGAACATGAGATTCCAGTGGTTCT TATCGGCCAGTGCGAGCTTGTCGCTGTTCCTTCTCCTGGACTTCGTCGTATGGATTCACGGCGAGCGGGACTTCAATGTC AACGACTCCCAGGTCCCTGTAATTGAGCCAAAGGATGTGCCCGCCTACAAACAGGATCCGTATGTTACGGAATTGATGAG TTGCCAAAATACTCCGAGTGAAATTGTGCTTTCACTTTTGTTGAAGAAGCACGACTGGAGTGAGCTGACGGCCACGAAAC GAGCTCATGTCCAGGCCAAGTTGGCCAAGTTCTTTGCCATACCCAAGGAATTCATTTCCCTGGATTCGGTGTCTAAACGC GAACTGAGATCCATGCACAAGTTGGCCATGAGAAAAGGAGGCAAGGGCAACAAGAATATCGAGACCCTGAATCGTCGCCT GGGACGCGCCAGTTTCATGATCGGCTGTGGTCCAAGCTACTTTGTGATGGGCGAACCGATAGCCAAGCAGATTGCCCACC AAATGAAGGATGGCACCATTGGCGCTTTGACGGAGGAGAACTTCGGGCTGTGGTTCATTTGGCGAAAGGAATTGAAATCA AGATCAAACCGCAAGCGACGTCAATCCGAGGGCTCTGGTGCCGATGAAGATGACTACGACTATGGGGATGACGATGAGGA AGTTGCTGAGCCCAGTACGGAAGTGCCACCAGTGACCACACATGCCCATCGACATCATCACGGAGCGCCAAATACTGTGC TTAATGAGCTCGAGCTGAGCACCCAGCTGCCCATCGACGATTTTGAAGGCACTGCTACTGCAACACAGCCAACTGGCACT GCAACTGCAACTGCCATTGCAAGCACAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAACCGCAACAGCCACAACTGC CAATGCATCTGCAACAACAGCAGGCGAAAGCGAATTTCATATAGAGTCCACAACGCACCGCACGATTAGCTCTAAGGAAG GAGGAGAACCCGATGCCATCAGCAGCGGCAACAATAGCCTGGCCAATAATGAGCTGTCGACGCCCGCCACGCCCACATCT TCGGTGGCCACGACCACGGCATCTACTCCGGAGTCGAGCAGCATCAGCAGCAACTTTGTCTCCACCGACTACATGGAACC GCAGCCGGAAGAGAATAGTCCGCCCATCATCAAGACACGCCTGCAGAAGCTGGCTGTCACATCGGGCAAGGCCTTCACCT TCCATGTGCTACCGGAAACGTTTTACGATGCCGAGGATCAGGGTAATCTCCGCCTGGCGCTCACCGACAAGGATGGCCAC GAGCTAAAGGCGAACTCTTGGCTGCAGTTTAACGCCGACAAGAGGGAGCTTTATGGCCTACCTCTGGACGACACGGTATC CCGCTGGCAATATCGCCTGTCGGCCACGGATTCGGGCAACGCCAGTGTCACGGAAACCGTGGAGATAAGTGTGCAGCAGC ATCGAGCGGTTAGGACCATTAACCACGAGATCAGTGTTTTTGTACGGATCAACGAGAAGCCGGGTCACAACATTGATTGG CAGTTGAAGCTCATTAATGCGGTGGCCAGAACCTTGGATGACTCCACCAATTCGGCAGTGGTGGTGCGTGAGATCCGACA GACACCACACGATCCACACAGTGCCACCTTCGTGTATTTTAATGAAACGCTGCCCACCAGCGAGTGTCCCGAAAAGGAAC TAAAGGATATTATTGCACGACTGGATGCCAATAGACTGAGTGATTTGGTTCAGCCACAGCTGGGAATCAAATCTATAACA GGACAGTTAATCGGATCCTGTCAGAAGGATTTAACTCAGGTGAAGCCCACGCAACACATGACCAAGAATGTGCCTCCAAT GCCACGCAACCAGGTGGATCGCGTTAACGCTAGCTTGGGCCAGCTGCTGGTCTACAAAGTGCCGGCGGATACCTTCTATG ATGCGAACGACAATCAATTGACTCTTACGTTGAAAACCAGGGATCACCTTGAGCTTAGCCCACGCCACTGGCTGCAGTTC GACTCCAAGAACGAAGAGTTCTATGGCATTCCGAAAAGCGGTGACATTGGTTCCGAGGAATATCTGTTGGTCGCCGAAGA CAGTGGTGGCCTGAGTGCCCATGATGCCCTGGTTGTAGTGGTTAGCCCAGCGCCAAAGCGTGACTTTGGGTTCTTCTTCA AAGCGTATCTGTCTATCAAACACGAGCGATTCAATGCCGATCTGCAGCGGAAGTTTGTAGAGCGTGTAGCGAAGCTGAAT GGAGATCCTACTACTGGCCAGATCCAGATCCGTTCGATAACCACGCACCACGACTCCGATGGCACCATAGTGAACTTCTA CAATACGACGCTGTACAGGAAGCACAACAGTTGTCGGGAGAAGGAAGTGGCCATGACTAGGAGTGTCTACCTAAACAGTG ACCTCAGCCTGAGGGAGGCAGCCAAACGGGCTCTGGGACCCGAGCTCAATTTGACCAACTTTTCGGTGGTGCCCTTCAGT ATTTGTCATCATACTGAGAACATAGACACCAACCAACTTGATTATATACCTAGTCGCCCCGAAGAGCCTACACATAAGTC CTCTTTCGGCGAGGATTACATGATCACGTTCGTATGGCCCATCGTGATCATTGTGGCGATGCTTGTGGCTGCCTCAATCA TTGCCTGTTGCCTGCACTGGTGTCGCCAAAGGAGCGGCAAAATGGAGCTAGGCGACGAAGAGGAGCGCAAGTCCTTCCGT GCCAAGGGTATTCCCGTCATCTTTCAGGACGAGTACGAGGAGAAGCCCGAGATCGGCAACAAGAGTCCTGTCATACTCAA GGACGAGAAGCCGCCGCTGCTGCCACCATCCTACAATACCTCAAACATGAATGGTGACAACGACGTGGACGATTATGTGC CACCACCATCGGTGGTTGTGGGCGGCCGGGAAGTGCGTGGCAAGTCGCCAGCCACGCCCTCCTACCGCAAACCACCGCCA TATGTGTCGCCATAAATCAGTGTCAAGAGCAGCAGGAAAGCAGCAAACCAGATTGGAAGCAACTGCAACAATAATAACAA TCGCATCGTATTAACCACACAATCTACATAGATATATACATATATATATTTAACCGATTTGGCACAAGTTATATGCAACT AAGCAAACTCTTTTGTATATATCCAAGTGCAAATTGGTTTCTTTGGACACTGTATTCGAATTGAGGGAAACTGCATCCCA GCCGAGAAGTATTCGCCAAGTCGATGGAGTTTTTTTATGGATAACGTTTTGTATTAGCCGAGAATGGAGAAACAATCATG TGCTAAATTACTGGCAAACATAAAGTCTACACCGTTGGACTATCGAGTATCACTAACACAAAACACAAATCGATGAACTA TGCTGCATATTATTTATTTAGTATGTACTCTAATTTATATGTAAACCACGCGAACCACAAGGACACAAATGGTGTTTGTT AGGACACAAGCATCGGAGCTTGAAAAACACACAGACACATGCACACGAGCCCATTTTAAGCATAGCATTTAAAACTCTTT GTACTAAAATTAAGTTAAACGCGAGAAAGAAGGCACACATGTCCATCGTCCCACGTCCCTGGGCAGCTATTAGACTTTCA GCTTGATTTGCTCAAGCTGAATTGCTTGCATGTTTTTTTTTTTTGAATTATCGGACCACTATGCTGCATATTTCCATCAA AGGATCAGGGGAACCACCGAGGCAGGCTGCGTACTTGCCGCTGTTTGGTGCCCAATTGAGTTTAGTACTTTAGTTGTTTT ACCATTGGCTTAACTTACTGGGACGTACGGCGAAGGACGTGTCCGAAAGGAGCTTGATTATTTTACATTCTAAGAAGCAA TCGAACCCACAATAGTGCTGCCAATAATCCAGGTACTCGGATCGAACTAGCCGTTTGAATACCAAACGATTCCTAATTAC CCACAAGTGCTTCCCACAGACAGACAAACCAACAGACCATATCCACCAGACACACAACATACAACATACAACACACACAC ACACAAATATTTATACGAAAATTGATTACTTAAAGTAGAATCTAGTTTTAAATGATTTTCACAAGCTCCCCTTTGGATAA TTCTATGTGAAATGGTTAGATTAGTTTATATGTAGCTGCTAGTTGGGCAGTACTTACTACACTACTACCTACTACTATCT GCTACCTAATCCAACCGTCCCTTATCCCTGAATCGAACTAACATGTAGTGAGTAAAGCATTGATTTTATATGAACTTATA GTGGAAGTCCAGAGCCTAAGTTAGAGCAATAGTTGCATGAAGGATGGGAAATGAAATTGAAATGGAAATTGAACTTGAAT ATGAAATTGAATTACTTTACGAGAGGACACTGATTTTGTCGTCGTGAAAATGTGCACGAAAGTGCTACGCAATTGCCTAT TAAACTAAGTACGATTAACTAATTTGTGTATTTGCATAAGCTCAACCGCTGAATTATTTACATTATGTAATTTGTAATCG AGCAAACAGCGGTCGCATTAGCAACAACAACAACAACAGCAACAGCAGCAATAACAACAAACGGTATCAATTTATTTTGA ATAAAAAAAATGCCAAAGATACGATTTTAGTTGCATTATTATTTGCCAATATTTGCGAATATTTTTTTAGACAAACAAAA ACACACACACACGAGAAAACACATAGACAAGAGTAAGTACAGAGCTGGAAAATTGTAATTTTTCTCCAAATCAAATAAAT AATTGTGCATATTTGTGAAAAATTGACCGAATGGCCATGGTCGAGTTGCACGTTTAATTAATAGATATATATATAGATAT ATATATTCAGTAAAAATCAGAGAAACTATTGTATATTTAATGCAATCTGTTTGCCATTCGATTGGCTTCAATTGAGCGAG CTGTACCCTCCACAAATACACAGGCAAATCAATAAACACATGCAATAATTATAATAATTATTGAGAATAACTAACTGCGT GAATATAAAGGTACATACATACATACGTACGTATGTACATACATATGTCCGTATAGGATATACGTATGCATATATACATG GATTCTAATAAACATTTATATATTTTGATACAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.
Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.
