FB2024_03 , released June 25, 2024
Clone: Dmel\AM02208
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General Information
Symbol
Dmel\AM02208
Species
D. melanogaster
Name
AM02208
FlyBase ID
FBcl0000903
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG34039
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    AM_cDNA
    • cDNA sequences of D. melanogaster, iso-1 strain, mixed stages.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      organism

      Comment: 0-24 hr AEL

      (Yandell et al., 2005)
      third instar larval stage
      organism
      (Yandell et al., 2005)
      pupal stage
      organism
      (Yandell et al., 2005)
      adult stage
      organism
      (Yandell et al., 2005)
      Sequence Data of the Insert
      3' Sequence
      Total bases
      168
      NCBI
      CX309426
      3' sequence dataSequence Downloader
      TGCCGCAGCCATGTGCAGAGTCCTCCAAAAGCANTNCTNNNCCATAAGGCAAACACTTATAAACAAATCTTTCAGAATTA
ACGAACTGACAAGCGTCCGAGACATATCTACGCCAGGGCAAACGCCTCCAAACTACGCCACCTTGGAACTGAAGATCTCG
CAAGAACT
      5' Sequence
      Total bases
      181
      NCBI
      CX309480
      5' sequence dataSequence Downloader
      GTTTGCCCTGGCGTNNNNTGTCTCGGACGCTTGTCAGTTCGTTAATNNNNNNNNATTTGTTTATAAGTGTTTGCCTTATG
GTGGCGGAATGCTTTTGGAGGACTCTGCACATGGCTGCGGCATGGTCCTTGGGAAGTCCCAATTGCTGGATCTCCTCGCC
AAAAATGCTTTCACTACAGCT
      Library Information (1)
      Library: AM_cDNA
      Description

      RT-PCR products amplified with pairs of gene-specific primers from template RNA representing pooled Drosophila stages.

      (Yandell et al., 2005)
      Sample preparation

      0-24 hr embryo (derived from 4-6 hr subcollections), late third-instar larva (L3), mixed-stage pupa, and mixed-age adult were pooled.

      Protocol

      Poly(A)+ RNA was isolated. RT-PCR products were amplified from template RNA using pairs of gene-specific primers.

      (Yandell et al., 2005)
      Comment

      This collection of ESTs resulted from direct sequencing of RT-PCR products and were not cloned into a vector are therefore unavailable as reagents.

      (Yandell et al., 2005)
      Reported As
      Symbol Synonyms
      AM
      AM_cDNA
      References (1)
      Research paper (1)
      Yandell et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(5): 1566--1571
      A computational and experimental approach to validating annotations and gene predictions in the Drosophila melanogaster genome. [FBrf0183655]
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)