FB2024_03 , released June 25, 2024
Assay: AM_cDNA
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General Information
Name
AM_cDNA
Species
D. melanogaster
Assay type
FlyBase ID
FBlc0000022
Project
Data Provider
Title
cDNA sequences of D. melanogaster, iso-1 strain, mixed stages.
Accessions
    Biosample Source
    Overview
    Strain
    iso-1
    (Yandell et al., 2005)
    Stage
    larval stage
    (Yandell et al., 2005)
    pupal stage
    (Yandell et al., 2005)
    adult stage
    (Yandell et al., 2005)
    Sex
    Tissue isolated
    organism
    (Yandell et al., 2005)
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    Sample preparation

    0-24 hr embryo (derived from 4-6 hr subcollections), late third-instar larva (L3), mixed-stage pupa, and mixed-age adult were pooled.

    Biosamples used (1)
    Biosample
    Type
    Title
    BS_AM_cDNA
    whole organism
    D. melanogaster, iso-1 strain, mixed stages, source for cDNA.
    Assay Details
    Methods
    poly(A) RNA isolation
    (Yandell et al., 2005)
    Key genes
    Protocol

    Poly(A)+ RNA was isolated. RT-PCR products were amplified from template RNA using pairs of gene-specific primers.

    (Yandell et al., 2005)
    Mode of Assay

    Products were directly sequenced from both ends with the same oligos used to prime PCR.

    (Yandell et al., 2005)
    Comments

    This collection of ESTs resulted from direct sequencing of RT-PCR products and were not cloned into a vector are therefore unavailable as reagents.

    (Yandell et al., 2005)
    Associated Data
    Size
    Associated features
    84 Clone(s)
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    Files
    Additional Information
    Synonyms and Secondary IDs (3)
    Reported As
    Symbol Synonym
    AM
    AM_cDNA
    Name Synonyms
    cDNA sequences of D. melanogaster, iso-1 strain, mixed stages.
    (Yandell et al., 2005)
    Secondary FlyBase IDs
      References (1)