A 1045bp Mst27D upstream fragment drives expression of amino acids 1-150 of the Mst27D coding sequence (encodes the N-terminal calponin homology domain), tagged at the C-terminal end with mCherry. An SV40 terminator is present.
Mst27DCH.mCherry fails to rescue Df(2L)ade3/Mst27DLL01793