The snRNA:U6:96Ac promoter drives expression of two sgRNAs, both of which target Tbc1d8-9 (target sequences are CTACTACAAGGATAAAGTGG and CGTGGCCATGGGGAATCACG).
Rab5Q88L.UASp.YFP, Scer\GAL4Ugt36A1.PK, Spyo\Cas9UAS.P2, Tbc1d8-9U6.gRNA.A has nephrocyte phenotype
Rab5Q88L.UASp.YFP, Scer\GAL4Ugt36A1.PK, Spyo\Cas9UAS.P2, Tbc1d8-9U6.gRNA.A has intracellular vesicle phenotype