UASp regulatory sequences drive expression of Graf cDNA (BDGP clone LD28528 with correction of three mutations back to wild-type) with deletion of the RhoGAP domain (residues 1177-1764). Tagged at the C-terminal with GFP.
Scer\GAL4VP16.nanos.UTR/GrafΔRhoGAP.UASp.EGFP fails to rescue GrafCR57