UASt regulatory sequences drive expression of full-length rhea (amino acids 1-2836, corresponding to the rhea-PB, rhea-PE and rhea-PF isoforms), that has been mutated to carry the amino acid substitutions T150A and T152A (non-phosphorylatable mutations). The coding sequence is tagged at the N-terminal end with three copies of Tag:FLAG.
Stage 16/17 rhea13-8 embryos show a 'rounded-up' muscle phenotype in which all muscles are detached from one another and from the cuticle. Upon Scer\GAL4Mef2.PU-driven expression of rheaT150A.T152A.UAS.Tag:FLAG (1 or 2 copies) muscle are still detached from the cuticle but not from one another; in addition, striations were lost, suggesting defects in myofibril assembly.
FBal0356120:, rhea13-8 is partially rescued by Scer\GAL4Mef2.PU, rheaT150A.T152A.UAS.Tag:FLAG/rheaT150A.T152A.UAS.Tag:FLAG
Scer\GAL4Mef2.PU/rheaT150A.T152A.UAS.Tag:FLAG partially rescues rhea13-8
Stage 16/17 rhea13-8 embryos show a 'rounded-up' muscle phenotype in which all muscles are detached from one another and from the cuticle. Upon Scer\GAL4Mef2.PU-driven expression of rheaT150A.T152A.UAS.Tag:FLAG (1 or 2 copies) muscle are still detached from the cuticle but not from one another; in addition, striations were lost, suggesting defects in myofibril assembly.