A sequence cassette has been introduced into the attP site present in TI{TI}DATattP, restoring the deleted DAT sequence and tagging isoform A with Tag:V5. The tag is followed by a 2A-lexA::p65 cassette, resulting in the lexA::p65 driver being expressed as a separate protein under the control of the endogenous DAT regulatory sequences (only the expression of isoform A is trapped by lexA::p65). A single loxP site is present downstream of the driver sequence (markers present in the progenitor insertion and donor plasmid have been removed using P1cre).