FlyBase curator comment: TI{2A-lexA::p65} represents a DNA segment which contains a viral 2A-like peptide followed by sequence encoding the lexA::p65 driver and which has been inserted into the genome via any technique (such as CRISPR/Cas9 or other targeted mutagenesis) that results in the inserted DNA not being flanked by transposable element ends. This sequence can act as a promoter trap element when it is inserted into an exon of an endogenous locus; if the insertion is in the correct orientation, the lexA::p65 driver will be expressed under the control of the regulatory sequences of the trapped gene. The presence of the viral 2A-like peptide (which promotes ribosomal 'skipping') ensures that the lexA::p65 driver is translated independently and not as a fusion protein with any upstream coding sequence of the endogenous locus. To prevent translational fusion of the lexA::p65 driver with any downstream coding sequence of the trapped gene, a stop codon may also be present in the inserted DNA sequence, or the sequence may be inserted just upstream of an endogenous stop codon. Other sequences may be present in the inserted DNA and the precise sequence inserted may differ for different insertions of the construct. For details of what is inserted into the genome for an individual insertion of TI{2A-lexA::p65}, see the description in the insertion report and in any allele reports associated with that insertion.