An sfGFP tag has been inserted into the endogenous cac locus. The tag (flanked by flexible linkers) is inserted at the translational start site of 14 of the 15 annotated cac isoforms, and is incorporated in-frame within the longer N-terminal cytoplasmic domain of the remaining isoform (cac-RN), thus all isoforms of the cac protein are expected to be tagged.
cacsfGFP-N homozygotes are capable of effective flight; their larval neuromuscular junction exhibit normal neurotransmission (i.e. Ca[2+] influx to low or high frequency stimulation, EJP and mEJP amplitudes and quantal content).