FB2024_03 , released June 25, 2024
Allele: Dmel\aPKCas4
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General Information
Symbol
Dmel\aPKCas4
Species
D. melanogaster
Name
FlyBase ID
FBal0346247
Feature type
allele
Associated gene
Dmel\aPKC
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Hannaford et al., 2019)
gene targeting by homologous recombination
(Hannaford et al., 2019)
Progenitor genotype
Cytology
Description

Amino acid replacement: I342A.

(Hannaford et al., 2019)

Encodes an analogue-sensitive form of the aPKC kinase; the kinase activity of the encoded protein can be specifically inhibited by addition of the 1NA-PP1 ATP analogue, while in the absence of this analogue aPKCas4 flies are viable and fertile (in vitro experiments also show that in the absence of the analogue, a protein carrying the two mutations found in aPKCas4 has comparable kinase activity as the wild-type protein).

(Hannaford et al., 2019)

Amino acid replacement: T405A.

(Hannaford et al., 2019)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
MNV
2R:14,948,291..14,948,292 [-]
Nucleotide change:

AT14948292GC

Reported amino acid change:

I342A

Comment:

Inferred nucleotide change from AT to GC. Site of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Hannaford et al., 2019)
point_mutation
2R:14,948,103..14,948,103 [-]
Nucleotide change:

A14948103G

Amino acid change:

T405A | aPKC-PA; T323A | aPKC-PB; T405A | aPKC-PC; T405A | aPKC-PD; T323A | aPKC-PE; T302A | aPKC-PF; T750A | aPKC-PG; T323A | aPKC-PI; T757A | aPKC-PJ; T318A | aPKC-PK; T302A | aPKC-PL; T302A | aPKC-PM

Reported amino acid change:

T405A

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Hannaford et al., 2019)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Upon application of 1NA-PP1 in ex vivo preparations, aPKCas4/aPKCas4 follicle cells, embryonic and larval neuroblasts show cell polarity defects, paralleled by the mechanical constriction of entire egg chambers, embryonic epithelium, eye discs and larval neuroepithelium. Unlike wild-type controls, the orientation of the division plane is highly variable between cell cycles in treated larval neuroblasts.

      (Hannaford et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      aPKCas4
      (Tokamov et al., 2024, Tokamov et al., 2023, Osswald et al., 2022, Ventura et al., 2020)
      apkcas4
      (Hannaford et al., 2019)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)