FlyBase Allele Report: Scer\GAL4[DBD.wrapper.932i]

General Information

Symbol

Scer\GAL4^{DBD.wrapper.932i}

Species

S. cerevisiae

Name

FlyBase ID

FBal0344748

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{wrapper-GAL4.DBD.932i}

Also Known As

wrapper932i-Gal4DBD

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Coutinho-Budd et al., 2017)

Progenitor genotype

Carried in construct

P{wrapper-GAL4.DBD.932i}

Cytology

Description

932bp fragment from wrapper ( 2R:18267450-18268381 from BAC CH322-90D04, corresponding to the 1st intron of the wrapper gene used in P{GMR54H02-GAL4}) drives expression of a GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Coutinho-Budd et al., 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR54H02

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 12

embryonic/larval cell body glial cell

Comment: when combined with P{nrv2-VP16.AD}

(Coutinho-Budd et al., 2017)

embryonic stage 15

embryonic/larval cell body glial cell

Comment: when combined with P{nrv2-VP16.AD}

(Coutinho-Budd et al., 2017)

first instar larval stage

embryonic/larval cell body glial cell

Comment: when combined with P{nrv2-VP16.AD}

(Coutinho-Budd et al., 2017)

second instar larval stage

embryonic/larval cell body glial cell

Comment: when combined with P{nrv2-VP16.AD}

(Coutinho-Budd et al., 2017)

third instar larval stage

embryonic/larval cell body glial cell

Comment: when combined with P{nrv2-VP16.AD}

(Coutinho-Budd et al., 2017)

adult stage

adult brain cell body glial cell

Comment: when combined with P{nrv2-VP16.AD}

(Coutinho-Budd et al., 2017)

Additional Information

Statement

Reference

Marker for

Reflects expression of

wrapper

(Coutinho-Budd et al., 2017)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | third instar larval stage, with Hsim\VP16^AD.nrv2, spz3^KK106772

(Coutinho-Budd et al., 2017)

abnormal locomotor behavior | third instar larval stage, with αSnap^KK107910, Hsim\VP16^AD.nrv2

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with CG9702^GD3131, Hsim\VP16^AD.nrv2

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Cka^JF01432, Hsim\VP16^AD.nrv2

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Cka^KK100338, Hsim\VP16^AD.nrv2

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, fry^GD10439

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, fry^KK101368

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Nsf2^GD1327

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Nsf2^HMS01262

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Nup98-96^GD6897

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Nup98-96^KK100388

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab1^JF02609

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab1^S25N.UAS.YFP

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab5^HMS00147

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab5^JF03335

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab5^KK102895

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab5^{S43N.UASp.YFP}

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab11^GD11761

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab11^JF02812

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab11^KK108297

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab11^{S25N.UASp.YFP}

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab35^JF02978

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab35^KK108660

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab35^S22N.UAS.YFP

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, spz3^GD4913

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, spz3^KK106772

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Syx5^JF03330

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Hsim\VP16^AD.nrv2, Syx5^KK100952

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with αSnap^JF03266, Hsim\VP16^AD.nrv2

(Coutinho-Budd et al., 2017)

abnormal neuroanatomy | third instar larval stage, with αSnap^KK107910, Hsim\VP16^AD.nrv2

(Coutinho-Budd et al., 2017)

chemical sensitive | adult stage, with CtsL1^GD5803, Hsim\VP16^AD.nrv2

(Lee et al., 2019)

chemical sensitive | adult stage, with CtsL1^HMS00725, Hsim\VP16^AD.nrv2

(Lee et al., 2019)

decreased cell number | third instar larval stage, with αSnap^KK107910, Hsim\VP16^AD.nrv2

(Coutinho-Budd et al., 2017)

increased cell death | third instar larval stage, with Hsim\VP16^AD.nrv2, spz3^KK106772

(Coutinho-Budd et al., 2017)

increased cell death | third instar larval stage, with αSnap^KK107910, Hsim\VP16^AD.nrv2

(Coutinho-Budd et al., 2017)

Phenotype Manifest In

embryonic/larval astrocyte-like glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, spz3^KK106772

(Coutinho-Budd et al., 2017)

embryonic/larval astrocyte-like glial cell | third instar larval stage, with αSnap^KK107910, Hsim\VP16^AD.nrv2

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with CG9702^GD3131, Hsim\VP16^AD.nrv2

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Cka^JF01432, Hsim\VP16^AD.nrv2

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Cka^KK100338, Hsim\VP16^AD.nrv2

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, fry^GD10439

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, fry^KK101368

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Nsf2^GD1327

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Nsf2^HMS01262

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Nup98-96^GD6897

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Nup98-96^KK100388

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab1^JF02609

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab1^S25N.UAS.YFP

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab5^HMS00147

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab5^JF03335

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab5^KK102895

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab5^{S43N.UASp.YFP}

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab11^GD11761

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab11^JF02812

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab11^KK108297

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab11^{S25N.UASp.YFP}

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab35^JF02978

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab35^KK108660

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Rab35^S22N.UAS.YFP

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, spz3^GD4913

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, spz3^KK106772

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Syx5^JF03330

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with Hsim\VP16^AD.nrv2, Syx5^KK100952

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with αSnap^JF03266, Hsim\VP16^AD.nrv2

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell | third instar larval stage, with αSnap^KK107910, Hsim\VP16^AD.nrv2

(Coutinho-Budd et al., 2017)

larval ventral nerve cord | third instar larval stage, with Hsim\VP16^AD.nrv2, spz3^KK106772

(Coutinho-Budd et al., 2017)

larval ventral nerve cord | third instar larval stage, with αSnap^KK107910, Hsim\VP16^AD.nrv2

(Coutinho-Budd et al., 2017)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

αSnap^KK107910, Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i} has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Ncra\QF^QF2.nSyb/spz3^QUAS.EGFP

(Coutinho-Budd et al., 2017)

NOT suppressed by

Statement

Reference

αSnap^KK107910, Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i} has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Ecol\lexA^GMR75E05/drpr^RNAi.lexAop

(Coutinho-Budd et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

αSnap^KK107910, Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i} has embryonic/larval cell body glial cell | third instar larval stage phenotype, suppressible | partially by Ncra\QF^QF2.nSyb/spz3^QUAS.EGFP

(Coutinho-Budd et al., 2017)

NOT suppressed by

Statement

Reference

αSnap^KK107910, Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i} has embryonic/larval astrocyte-like glial cell | third instar larval stage phenotype, non-suppressible by Ecol\lexA^GMR75E05/drpr^RNAi.lexAop

(Coutinho-Budd et al., 2017)

αSnap^KK107910, Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i} has larval ventral nerve cord | third instar larval stage phenotype, non-suppressible by Ecol\lexA^GMR75E05/drpr^RNAi.lexAop

(Coutinho-Budd et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

spz3^UAS.RR.EGFP, Scer\GAL4^{DBD.wrapper.932i}, Hsim\VP16^AD.nrv2 partially rescues spz3^KK106772

(Coutinho-Budd et al., 2017)

spz3^UAS.EGFP, Scer\GAL4^{DBD.wrapper.932i}, Hsim\VP16^AD.nrv2 partially rescues spz3^EY06670

(Coutinho-Budd et al., 2017)

Fails to rescue

spz3^UAS.EGFP, Scer\GAL4^{DBD.wrapper.932i}, Hsim\VP16^AD.nrv2 fails to rescue spz3^KK106772

(Coutinho-Budd et al., 2017)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^{DBD.wrapper.932i}

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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