UASp regulatory sequences drive expression of the lola-F isoform coding sequence. The protein is tagged at the N-terminal end with Tag:FLAG.
abnormal neuroanatomy | embryonic stage, with Scer\GAL4elav-C155
axon | embryonic stage, with Scer\GAL4elav-C155
larval ventral nerve cord | embryonic stage, with Scer\GAL4elav-C155
Expressing lolaUASp.F.Tag:FLAG under the control of Scer\GAL4elav-C155 results in disrupted axonal tracks in the ventral nerve cord of stage 15/16 embryos.
Scer\GAL4elav-C155/lolaUASp.F.Tag:FLAG rescues lolaF.stop
Scer\GAL4elav-C155/lolaUASp.F.Tag:FLAG rescues lolaF.znf
Scer\GAL4Tdc2.PU/lolaUASp.F.Tag:FLAG fails to rescue lolaKO.O