FB2024_03 , released June 25, 2024
Allele: Hsap\LAMP2UAS.cIa
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General Information
Symbol
Hsap\LAMP2UAS.cIa
Species
H. sapiens
Name
UAS construct a of Issa
FlyBase ID
FBal0343214
Feature type
allele
Associated gene
Hsap\LAMP2
Associated Insertion(s)
Carried in Construct
P{UAS-hLAMP2.I}
Key Links
Transgenic product class
cDNA
(Issa et al., 2018)
wild_type
(Issa et al., 2018)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Issa et al., 2018)
wild_type
(Issa et al., 2018)
Mutagen
in vitro construct
(Issa et al., 2018)
Progenitor genotype
Carried in construct
P{UAS-hLAMP2.I}
Cytology
Description

UASt regulates expression of the Hsap\LAMP2 cDNA (clone H05D091G15. (The sequence upstream of the ATG initiation codon was modified to match the Drosophila translation start consensus sequence.)

(Issa et al., 2018)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Hsap\LAMP2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Individuals expressing Hsap\LAMP2UAS.cIa under the control of Scer\GAL4elav-C155 develop normally to adults and do not show apparent behavioral defects. Adults show longer lifespan upon starvation or paraquat-induced oxidative stress, and increased climbing ability but no change in lifespan under control conditions. These individuals show significantly increased numbers of KFERQ-positive puncta (marker of late endosomes and lysosomes) in the adult brain under fed conditions, but not under starvation, nor in the larval fat body under fed or starvation conditions.

      (Issa et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\LAMP2UAS.cIa
      Name Synonyms
      UAS construct a of Issa
      Secondary FlyBase IDs
        References (2)