UAS regulatory sequences drive expression of an shRNA construct that targets sisRNA:3 for RNAi-mediated degradation. (Cloned into the 'Valium22' plasmid.)
Individuals expressing sisRNA:3dsRNA.shRNA.UAS.V22.1 under the control of Scer\GAL4da.PU develop normally during embryogenesis and hatch at similar rates to controls but show a significant delay in pupa maturation.