Blocking protein synthesis (using Rcom\RA[cs2.UAS] at 30[o]C) for the full 24 h, or the first 12 h, but not the first 8 or 10 h, nor other 12 h intervals, of the 24 h between spaced, but not massed, training and testing in Scer\GAL4^BG01228 neurons, significantly impairs aversive olfactory long term memory in adults, compared to controls; there is no effect when protein synthesis is instead blocked for 24 h prior to training.

Blocking protein synthesis (using Rcom\RA[cs2.UAS] at 30[o]C) for the full 24 h between spaced training and testing in the combined set of Scer\GAL4^BG01228 neurons and Scer\GAL4^VT044170 neurons, or Scer\GAL4^BG01228 neurons and Scer\GAL4^VT016811 neurons, or Scer\GAL4^BG01228 neurons, Scer\GAL4^VT044170 neurons and Scer\GAL4^VT016811 neurons, significantly impairs aversive olfactory long term memory in adults, compared to controls, with similar effect sizes, which are similar to effect sizes seen with any single-driver set alone.

Blocking synaptic transmission (using shi^1.Scer\UAS at 32[o]C) from Scer\GAL4^GMR66C08 (but not Scer\GAL4^BG01228) neurons significantly abolishes naive odor avoidance behavior (to MCH or OCT at 1:1000 ), compared to controls.