FB2024_03 , released June 25, 2024
Allele: Dmel\GlosGD17786
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General Information
Symbol
Dmel\GlosGD17786
Species
D. melanogaster
Name
FlyBase ID
FBal0336670
Feature type
allele
Associated gene
Dmel\Glos
Associated Insertion(s)
Carried in Construct
P{GD17786}
Also Known As
Glos RNAi, UAS.Glos-IRDEX
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Lanfranco et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Lanfranco et al., 2017)
Mutagen
in vitro construct
(Lanfranco et al., 2017)
Progenitor genotype
Carried in construct
P{GD17786}
Cytology
Description

UASt regulates expression of an dsRNA construct targeting Glos for RNAi-mediated degradation.

(Lanfranco et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD17786
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of GlosGD17786 under the control of Scer\GAL4Mef2.PR has no effect on flight ability of adult flies, mobility of the third instar larvae or shape of pupae and Scer\GAL4elav-C155-driven expression does not impair adult viability.

      Expression of GlosGD17786, together with UAS-Dicer2 to enhance RNAi efficiency, is lethal at both 25[o]C or 29[o]C if driven by Scer\GAL4αTub84B.PL, Scer\GAL4da.G32, Scer\GAL4how-24B or Scer\GAL4c179 and at 25[o]C only if driven by Scer\GAL4insc-Mz1407 (but only partially lethal at 29[o]C). Adult viability is not significantly reduced if GlosGD17786 is controlled by Scer\GAL4RapGAP1-OK6, Scer\GAL4elav-C155, Scer\GAL4G7 or Scer\GAL4Mef2.PR. Scer\GAL4αTub84B.PL-, Scer\GAL4da.G32- or Scer\GAL4c179-driven expression is lethal at both temperatures (and at just 29[o]C with Scer\GAL4how-24B) even without the UAS-Dicer2.

      The climbing (especially at late adulthood) as well as the flight ability of flies expressing GlosGD17786 (with UAS-Dicer2) under the control of either Scer\GAL4Mef2.PR or Scer\GAL4elav-C155 is strongly reduced and the adult survival rate is also significantly decreased with the Scer\GAL4elav-C155 driver.

      (Lanfranco et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Gem3ΔN.Scer\UAS together with GlosGD17786 are adult lethal and leads to strongly reduced mobility in third instar larvae and more elongated shape of the puparium with the Scer\GAL4Mef2.PR driver and to reduced viability with Scer\GAL4elav-C155.

      (Lanfranco et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      GlosGD17786
      Name Synonyms
      Secondary FlyBase IDs
        References (4)