FB2024_03 , released June 25, 2024
Allele: Dmel\TBPHKO.hTARDBP.WT.DsR+
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General Information
Symbol
Dmel\TBPHKO.hTARDBP.WT.DsR+
Species
D. melanogaster
Name
FlyBase ID
FBal0335733
Feature type
allele
Associated gene
Dmel\TBPH
Associated Insertion(s)
TI{TI}TBPHKO.hTARDBP.WT.DsR+
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Chang and Morton, 2017)
gene targeting by homologous recombination
(Chang and Morton, 2017)
Progenitor genotype
Associated Insertion(s)
TI{TI}TBPHKO.hTARDBP.WT.DsR+
Cytology
Description

The endogenous TBPH coding region is replaced with fly codon-optimized Hsap\TARDBP cDNA with an N-terminal Tag:FLAG tag and a Disc\RFPDsRed.3xP3.cUa marker flanked by loxP sites at the C-terminal. This results in the Hsap\TARDBP sequence being expressed under the control of the endogenous TBPH regulatory regions.

(Chang and Morton, 2017)
Allele components
Component
Use(s)
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  amyotrophic lateral sclerosis type 10
      CEA
      (Lehmkuhl et al., 2021)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      TBPHKO.hTARDBP.WT.DsR+ homozygotes of either sex are viable and display no significant changes in developmental timing, longevity or fecundity compared to controls. Neither larval (distance travelled in 5min) not adult locomotion is perturbed (the adults show normal diurnal activity pattern and both the overall daily activity and climbing assay performance as they age is comparable to controls).

      (Chang and Morton, 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      TBPHKO.hTARDBP.WT.DsR+
      Name Synonyms
      Secondary FlyBase IDs
        References (4)