The VT008469 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).
Comment: when combined with P{VT019059-GAL4.DBD}
Comment: when combined with P{VT000926-GAL4.DBD} (combination referred to as 'SS46596')
Thermogenetic activation (using TrpA1UAS.cvPa at 30[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT019059 and Hsap\RELAAD.VT008469 results in a small, significant increase in the total distance, but not the bout distance, of backwards walking, with a significant decrease in forwards walking, compared to controls.
Inhibition (using Ctet\TeTxLCTNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT019059 and Hsap\RELAAD.VT008469 does not significantly affect backing-up behavior when adult flies reach a narrow dead end, compared to controls; there is no significant effect on walking direction in unimpeded conditions, compared to controls.