FlyBase Allele Report: Hsap\FUS[UAS.cMa]

General Information

Symbol

Hsap\FUS^UAS.cMa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Matsukawa

FlyBase ID

FBal0327464

Feature type

allele

Associated gene

Hsap\FUS

Associated Insertion(s)

Carried in Construct

P{UAS-hFUS.M}

Also Known As

FUS wt

Key Links

Transgenic product class

cDNA

(Matsukawa et al., 2016)

wild_type

(Matsukawa et al., 2016)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Matsukawa et al., 2016)

wild_type

(Matsukawa et al., 2016)

Mutagen

in vitro construct

(Matsukawa et al., 2016)

Progenitor genotype

Carried in construct

P{UAS-hFUS.M}

Cytology

Description

UAS regulatory sequences drive expression of full-length Hsap\FUS cDNA.

(Matsukawa et al., 2016)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\FUS

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis type 6

CEA

(An et al., 2022, Kishino et al., 2022, Matsumoto et al., 2018, Matsukawa et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of amyotrophic lateral sclerosis type 6

is NOT exacerbated by Hsap\PFN1^C71G.UAS

(Matsukawa et al., 2016)

is NOT exacerbated by Hsap\PFN1^UAS.cMa

(Matsukawa et al., 2016)

is NOT exacerbated by Hsap\PFN1^M114T.UAS

(Matsukawa et al., 2016)

is exacerbated by Hsap\FUS^ΔC.UAS

(Matsumoto et al., 2018)

is exacerbated by Hsap\FUS^R495X.UAS

(Matsumoto et al., 2018)

is ameliorated by Hsap\CSNK1D^UAS.Tag:HA

(Kishino et al., 2022)

is exacerbated by Hrb87F^RNAi.UAS.cUa

(An et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^GMR.PU

(Matsukawa et al., 2016)

decreased size | adult stage, with Scer\GAL4^GMR.PU

(Matsukawa et al., 2021)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PU

(Matsukawa et al., 2016)

retina, with Scer\GAL4^GMR.PU

(Matsukawa et al., 2016)

retina | adult stage, with Scer\GAL4^GMR.PU

(Matsukawa et al., 2021)

Detailed Description

Statement

Reference

The expression of Hsap\FUS^UAS.cMa under the control of Scer\GAL4^GMR.PU leads to the thinning of the adult retina.

(Matsukawa et al., 2021)

The expression of Hsap\TARDBP^Scer\UAS.cIa under the control of Scer\GAL4^GMR.PU leads to a significant decrease in the retinal thickness in 5 days old adults, as compared to controls.

(Matsukawa et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Hsap\FUS^UAS.cMa, Scer\GAL4^GMR.PU has abnormal size phenotype, non-enhanceable by Hsap\PFN1^UAS.cMa, Scer\GAL4^GMR.PU

(Matsukawa et al., 2016)

Hsap\FUS^UAS.cMa, Scer\GAL4^GMR.PU has abnormal size phenotype, non-enhanceable by Hsap\PFN1^C71G.UAS, Scer\GAL4^GMR.PU

(Matsukawa et al., 2016)

Hsap\FUS^UAS.cMa, Scer\GAL4^GMR.PU has abnormal size phenotype, non-enhanceable by Hsap\PFN1^M114T.UAS, Scer\GAL4^GMR.PU

(Matsukawa et al., 2016)

NOT suppressed by

Statement

Reference

Hsap\FUS^UAS.cMa, Scer\GAL4^GMR.PU has decreased size | adult stage phenotype, non-suppressible by Hsap\NEAT1^UAS.1, Scer\GAL4^GMR.PU

(Matsukawa et al., 2021)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Hsap\FUS^UAS.cMa, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by Hsap\PFN1^UAS.cMa, Scer\GAL4^GMR.PU

(Matsukawa et al., 2016)

Hsap\FUS^UAS.cMa, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by Hsap\PFN1^C71G.UAS, Scer\GAL4^GMR.PU

(Matsukawa et al., 2016)

Hsap\FUS^UAS.cMa, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by Hsap\PFN1^M114T.UAS, Scer\GAL4^GMR.PU

(Matsukawa et al., 2016)

NOT suppressed by

Statement

Reference

Hsap\FUS^UAS.cMa, Scer\GAL4^GMR.PU has retina | adult stage phenotype, non-suppressible by Hsap\NEAT1^UAS.1, Scer\GAL4^GMR.PU

(Matsukawa et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The retinal thinning observed in 5 days old adults upon the expression of Hsap\FUS^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PU is not exacerbated by the co-expression of Hsap\PFN1^Scer\UAS.cMa, Hsap\PFN1^{C71G.Scer\UAS} or Hsap\PFN1^{M114T.Scer\UAS}.

(Matsukawa et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\FUS^Scer\UAS.cMa

Hsap\FUS^UAS.cMa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Matsukawa

Secondary FlyBase IDs

References (5)

Report Sections

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