Imprecise excision of P{EPgy2}Rab2EY02998 results in a 2047bp deletion which removes most of the protein coding sequences of both predicted Rab2 isoforms.
Inferred position of a 2047 bp deletion resulting from the imprecise excision of P{EPgy2}Rab2EY02998 that removes most of the Rab2 coding sequences.
Rab2d42 mutants die as second or third instar larvae and the mutant larval fat body cells show deficit in the formation of autolysosomes upon starvation (as assessed by LysoTracker and other marker stainings): both the number and size of marker-positive dots is reduced compared to controls. Instead of the degrading autolysosomes, the Rab2d42 mutant cells accumulate double-membrane autophagosomes. Basal autophagic degradation in well-fed mutant larvae is also disrupted. The mutant larval nephrocytes also show defects in the endosomal trafficking and degradation: aberrant late endosomes accumulate in the cells and the total number of lysosomes as well as the proportion of acid phosphatase-positive lysosomes is reduced relative to controls.
Rab2d42 is rescued by Scer\GAL4Rab2.ATG/Rab2UAS.YFP
The larval lethality of Rab2d42 mutants can be rescued by expression of Rab2Scer\UAS.T:Avic\GFP-YFP under the control of Scer\GAL4Rab2.ATG in the mutant background.
