FlyBase Allele Report: Hsap\FUS[UAS.cIa]

General Information

Symbol

Hsap\FUS^UAS.cIa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Ishiguro

FlyBase ID

FBal0327174

Feature type

allele

Associated gene

Hsap\FUS

Associated Insertion(s)

Carried in Construct

P{UAS-hFUS.I}

Also Known As

UAS-FUS

Key Links

Transgenic product class

wild_type

(Ishiguro et al., 2017)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ishiguro et al., 2017)

Mutagen

in vitro construct

(Ishiguro et al., 2017)

Progenitor genotype

Carried in construct

P{UAS-hFUS.I}

Cytology

Description

UAS regulatory sequences drive expression of Hsap\FUS.

(Ishiguro et al., 2017)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\FUS

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis

CEA

(Ishiguro et al., 2017)

model of amyotrophic lateral sclerosis type 6

CEA

(Lo Piccolo et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of amyotrophic lateral sclerosis

is ameliorated by Zzzz\UGGAA^22.UAS

(Ishiguro et al., 2017)

ameliorates spinocerebellar ataxia type 31

modeled by Zzzz\UGGAA^W.UAS

(Ishiguro et al., 2017)

modeled by Zzzz\UGGAA^W.UAS, TBPH^GD6943

(Ishiguro et al., 2017)

modeled by Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

modeled by Zzzz\UGGAA^S.UAS, TBPH^GD6943

(Ishiguro et al., 2017)

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 1

modeled by Zzzz\GGGGCC^42.UAS

(Fujino et al., 2023)

modeled by Zzzz\GGGGCC^89.UAS

(Fujino et al., 2023)

model of amyotrophic lateral sclerosis type 6

is ameliorated by lncRNA:Hsrω^EP93D

(Lo Piccolo et al., 2017)

is ameliorated by lncRNA:Hsrω^RNAi.Sym.UAS

(Lo Piccolo et al., 2019, Lo Piccolo et al., 2017)

is ameliorated by csul^UAS.Tag:MYC

(Lo Piccolo et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PS

(Ishiguro et al., 2017)

eye | progressive, with Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

eye disc | third instar larval stage, with Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

Detailed Description

Statement

Reference

The expression of Hsap\FUS^Scer\UAS.cIa under the control of Scer\GAL4^GMR.PS leads to a mild rough eye phenotype, as compared to controls.

(Ishiguro et al., 2017)

The progressive eye degeneration and the associated rough eye appearance, ommatidial fusion and pigmentation loss characteristic for adult flies expressing Hsap\FUS^Scer\UAS.cIa under the control of Scer\GAL4^GMR.PS is accelerated at 28[o]C when it is clearly visible already at day 1 post-eclosion, whereas at 25[o]C the phenotype is not manifested until day 6. The eye degeneration phenotype is even stronger when two copies of the Hsap\FUS^Scer\UAS.cIa transgene are used and the flies cultured at 28[o]C - under these extreme conditions the ommatidial lattice is completely obliterated.

A significant increase in apoptosis (assessed by [*]Casp3 immunolabelling) is observed in third larval instar eye discs expressing Hsap\FUS^Scer\UAS.cIa under Scer\GAL4^GMR.PS.

(Lo Piccolo et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has visible | adult stage phenotype, non-enhanceable by Atg8a^d4/Atg8a[+]

(Lo Piccolo et al., 2017)

Suppressed by

Statement

Reference

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has visible | adult stage phenotype, suppressible | partially by Zzzz\UGGAA^22.UAS, Scer\GAL4^GMR.PS

(Ishiguro et al., 2017)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has abnormal eye color phenotype, suppressible | partially by Zzzz\UGGAA^22.UAS, Scer\GAL4^GMR.PS

(Ishiguro et al., 2017)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has visible | adult stage phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has increased cell death | third instar larval stage phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

Atg8a^d4/Atg8a[+], Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has visible | adult stage phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

NOT suppressed by

Statement

Reference

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has visible | adult stage phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has visible | adult stage phenotype, non-suppressible by Diap1^UAS.cHa, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has visible | adult stage phenotype, non-suppressible by Hsap\LAMP1^UAS.HRP, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

Suppressor of

Statement

Reference

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor of abnormal eye color phenotype of Scer\GAL4^GMR.PS, Zzzz\GGGGCC^42.UAS

(Fujino et al., 2023)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor of increased mortality during development phenotype of Scer\GAL4^GMR.PS, Zzzz\GGGGCC^42.UAS

(Fujino et al., 2023)

Hsap\FUS^UAS.cIa, Scer\GAL4^{elav.Switch.PO} is a suppressor of decreased rate of adult locomotory behavior | RU486 conditional phenotype of Scer\GAL4^{elav.Switch.PO}, Zzzz\GGGGCC^42.UAS

(Fujino et al., 2023)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor of decreased size | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\GGGGCC^89.UAS

(Fujino et al., 2023)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor of abnormal eye color phenotype of Scer\GAL4^GMR.PS, Zzzz\GGGGCC^89.UAS

(Fujino et al., 2023)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^W.UAS

(Ishiguro et al., 2017)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor | partially of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^W.UAS

(Ishiguro et al., 2017)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor | partially of abnormal eye color phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^W.UAS

(Ishiguro et al., 2017)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PS, TBPH^GD6943, Zzzz\UGGAA^W.UAS

(Ishiguro et al., 2017)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor | partially of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PS, TBPH^GD6943, Zzzz\UGGAA^W.UAS

(Ishiguro et al., 2017)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor of lethal phenotype of Scer\GAL4^GMR.PS, TBPH^GD6943, Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

TBPH^GD6943/Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

TBPH^GD6943/Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor | partially of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

TBPH^GD6943/Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor | partially of abnormal eye color phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

NOT Suppressor of

Statement

Reference

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a non-suppressor of abnormal eye color phenotype of Scer\GAL4^GMR.PS, Zzzz\poly-GR^PO-36.UAS

(Fujino et al., 2023)

Other

Statement

Reference

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS, Zzzz\poly-GR^PO-100.UAS has lethal phenotype

(Fujino et al., 2023)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has eye phenotype, non-enhanceable by Atg8a^d4/Atg8a[+]

(Lo Piccolo et al., 2017)

Suppressed by

Statement

Reference

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has eye phenotype, suppressible | partially by Zzzz\UGGAA^22.UAS, Scer\GAL4^GMR.PS

(Ishiguro et al., 2017)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has ommatidium | adult stage phenotype, suppressible | partially by Zzzz\UGGAA^22.UAS, Scer\GAL4^GMR.PS

(Ishiguro et al., 2017)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has retina | adult stage phenotype, suppressible | partially by Zzzz\UGGAA^22.UAS, Scer\GAL4^GMR.PS

(Ishiguro et al., 2017)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has eye phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has eye disc | third instar larval stage phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

Atg8a^d4/Atg8a[+], Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has eye phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

NOT suppressed by

Statement

Reference

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has eye phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has eye phenotype, non-suppressible by Diap1^UAS.cHa, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS has eye phenotype, non-suppressible by Hsap\LAMP1^UAS.HRP, Scer\GAL4^GMR.PS

(Lo Piccolo et al., 2017)

Suppressor of

Statement

Reference

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor of eye phenotype of Scer\GAL4^GMR.PS, Zzzz\GGGGCC^42.UAS

(Fujino et al., 2023)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor of eye phenotype of Scer\GAL4^GMR.PS, Zzzz\GGGGCC^89.UAS

(Fujino et al., 2023)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor of embryonic/larval salivary gland | larval stage phenotype of Scer\GAL4^GMR.PS, Zzzz\GGGGCC^89.UAS

(Fujino et al., 2023)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor of eye disc phenotype of Scer\GAL4^GMR.PS, Zzzz\GGGGCC^89.UAS

(Fujino et al., 2023)

TBPH^GD6943/Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor | partially of eye | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor | partially of eye | adult stage phenotype of Scer\GAL4^GMR.PS, Zzzz\UGGAA^W.UAS

(Ishiguro et al., 2017)

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a suppressor of eye | adult stage phenotype of Scer\GAL4^GMR.PS, TBPH^GD6943, Zzzz\UGGAA^W.UAS

(Ishiguro et al., 2017)

NOT Suppressor of

Statement

Reference

Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PS, Zzzz\poly-GR^PO-36.UAS

(Fujino et al., 2023)

Other

Statement

Reference

Hsap\FUS^UAS.cIa, Lamp1^MI15062/Lamp1[+], Scer\GAL4^GMR.PS, lncRNA:Hsrω^RNAi.Sym.UAS has eye disc | third instar larval stage phenotype

(Lo Piccolo et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The additional expression of Hsap\FUS^Scer\UAS.cIa leads to partial decreases in the severity of the eye defects either resulting from the Scer\GAL4^GMR.PS-driven expression of Zzzz\UGGAA^W.Scer\UAS alone or the combined expression of Zzzz\UGGAA^W.Scer\UAS and TBPH^GD6943 (i.e. reduced size, loss of pigmentation, defective morphology and ommatidial abnormalities).

The co-expression of Hsap\FUS^Scer\UAS.cIa rescues the lethality resulting from the co-expression of Zzzz\UGGAA^S.Scer\UAS and TBPH^GD6943 under the control of Scer\GAL4^GMR.PS; the rescued adults exhibit significantly less severe eye phenotypes, as compared to individuals only expressing Zzzz\UGGAA^S.Scer\UAS under the control of Scer\GAL4^GMR.PS.

The eye defects observed upon the expression of Hsap\FUS^Scer\UAS.cIa under the control of Scer\GAL4^GMR.PS are partially suppressed by the co-expression of Zzzz\UGGAA^22.Scer\UAS.

(Ishiguro et al., 2017)

The eye degeneration and the associated rough eye appearance, ommatidial fusion and pigmentation loss characteristic for adult flies expressing Hsap\FUS^Scer\UAS.cIa under the control of Scer\GAL4^GMR.PS is completely rescued by co-expression of lncRNA:Hsrω^{dsRNA.Sym.Scer\UAS} but not BacA\p35^Scer\UAS.cHa, Diap1^Scer\UAS.cHa) or Hsap\LAMP1^{Scer\UAS.T:Arus\HRP}. The complete rescue of the eye defects by lncRNA:Hsrω^{dsRNA.Sym.Scer\UAS} expression is however prevented by combination with a single copy of Lamp1^MI15062 as a vast majority of Lamp1^MI15062 heterozygotes expressing both Hsap\FUS^Scer\UAS.cIa and lncRNA:Hsrω^{dsRNA.Sym.Scer\UAS} under Scer\GAL4^GMR.PS display areas of degeneration in the eyes.

The eye degeneration induced by Hsap\FUS^Scer\UAS.cIa expression (one copy) under Scer\GAL4^GMR.PS is not exacerbated by combination with Atg8a^d4/+ and the eye defects in the Scer\GAL4^GMR.PS/Atg8a^d4;Hsap\FUS^Scer\UAS.cIa/+ flies can still be rescued by expression of lncRNA:Hsrω^{dsRNA.Sym.Scer\UAS} (one copy).

The extreme eye degeneration phenotype (complete loss of the ommatidial lattice), observed when two copies of Hsap\FUS^Scer\UAS.cIa are expressed and the flies kept at 28[o]C, is rather weakly suppressed by expression of one copy of lncRNA:Hsrω^{dsRNA.Sym.Scer\UAS} and more strongly when two copies are used but even then the rescue remains incomplete under these conditions.

The eye degeneration induced by Hsap\FUS^Scer\UAS.cIa expression (one copy) is not exacerbated by combination with Atg8a^d4/+ and the eye defects in the Scer\GAL4^GMR.PS/Atg8a^d4;Hsap\FUS^Scer\UAS.cIa/+ flies can still be rescued by expression of lncRNA:Hsrω^{dsRNA.Sym.Scer\UAS} (one copy).

The increase in the number of apoptotic ([*]Casp3-positive) cells in third instar larval eye discs observed upon Scer\GAL4^GMR.PS-expression of Hsap\FUS^Scer\UAS.cIa is partially suppressed by co-expression of lncRNA:Hsrω^{dsRNA.Sym.Scer\UAS}.

(Lo Piccolo et al., 2017)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\FUS^Scer\UAS.cIa

Hsap\FUS^UAS.cIa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Ishiguro

Secondary FlyBase IDs

References (4)

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