FlyBase Allele Report: Dmel\Cdk9[D199N.UAS.SR]

General Information

Symbol

Dmel\Cdk9^D199N.UAS.SR

Species

D. melanogaster

Name

FlyBase ID

FBal0327026

Feature type

allele

Associated gene

Dmel\Cdk9

Associated Insertion(s)

Carried in Construct

P{UAS-Cdk9.SR.D199N}

Key Links

Transgenic product class

missense_variant

(Liu et al., 2017)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Liu et al., 2017)

Mutagen

in vitro construct

(Liu et al., 2017)

Progenitor genotype

Carried in construct

P{UAS-Cdk9.SR.D199N}

Cytology

Description

UASt regulatory sequences drive a shmiRNA resistant (silent nucleotide mutations), kinase-dead (D199N) form of full-length Cdk9 cDNA (DGRC GH21935).

(Liu et al., 2017)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Cdk9

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Cdk9^{D199N.Scer\UAS.SR} under the control of either Scer\GAL4^insc-Mz1407 or Scer\GAL4^pnt-14-94 leads to third instar larval brain neuroblasts which do not exhibit obvious changes in cortical polarity or in number per brain lobe, as compared to controls.

(Liu et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of increased cell number | third instar larval stage phenotype of Scer\GAL4^insc-Mz1407, Su(Tpl)^{UAS.SR.Tag:MYC}, lilli^{UAS.SR.Tag:FLAG}

(Liu et al., 2017)

Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^insc-Mz1407, Su(Tpl)^{UAS.SR.Tag:MYC}, lilli^{UAS.SR.Tag:FLAG}

(Liu et al., 2017)

Cdk9^D199N.UAS.SR, Scer\GAL4^pnt-14-94 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^pnt-14-94, Su(H)^VP16.UAS

(Liu et al., 2017)

Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^insc-Mz1407, Su(H)^VP16.UAS

(Liu et al., 2017)

Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of increased cell number | third instar larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

E(spl)mγ-HLH^UAS.cLb/Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of increased cell number | third instar larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

E(spl)mγ-HLH^UAS.cLb/Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of increased cell number | third instar larval stage phenotype of E(spl)mγ-HLH^UAS.cLb, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of E(spl)mγ-HLH^UAS.cLb, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

Cdk9^D199N.UAS.SR, Scer\GAL4^pnt-14-94 is a suppressor of increased cell number | third instar larval stage phenotype of Scer\GAL4^pnt-14-94, Su(H)^VP16.UAS

(Liu et al., 2017)

Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of increased cell number | third instar larval stage phenotype of Scer\GAL4^insc-Mz1407, Su(H)^VP16.UAS

(Liu et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^pnt-14-94/Cdk9^D199N.UAS.SR is a suppressor of neuroblast | third instar larval stage phenotype of Scer\GAL4^insc-Mz1407, Su(H)^VP16.UAS

(Liu et al., 2017)

Scer\GAL4^insc-Mz1407/Cdk9^D199N.UAS.SR is a suppressor of neuroblast | third instar larval stage phenotype of Scer\GAL4^pnt-14-94, Su(H)^VP16.UAS

(Liu et al., 2017)

Scer\GAL4^pnt-14-94/Cdk9^D199N.UAS.SR is a suppressor of embryonic/larval brain | third instar larval stage phenotype of Scer\GAL4^insc-Mz1407, Su(H)^VP16.UAS

(Liu et al., 2017)

Scer\GAL4^insc-Mz1407/Cdk9^D199N.UAS.SR is a suppressor of embryonic/larval brain | third instar larval stage phenotype of Scer\GAL4^pnt-14-94, Su(H)^VP16.UAS

(Liu et al., 2017)

Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of type II neuroblast | third instar larval stage phenotype of Scer\GAL4^insc-Mz1407, Su(Tpl)^{UAS.SR.Tag:MYC}, lilli^{UAS.SR.Tag:FLAG}

(Liu et al., 2017)

Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of neuroblast | third instar larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

E(spl)mγ-HLH^UAS.cLb/Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of neuroblast | third instar larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of embryonic/larval brain | third instar larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

E(spl)mγ-HLH^UAS.cLb/Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of embryonic/larval brain | third instar larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of embryonic/larval brain | third instar larval stage phenotype of Scer\GAL4^insc-Mz1407, Su(Tpl)^{UAS.SR.Tag:MYC}, lilli^{UAS.SR.Tag:FLAG}

(Liu et al., 2017)

Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of neuroblast | third instar larval stage phenotype of E(spl)mγ-HLH^UAS.cLb, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of embryonic/larval brain | third instar larval stage phenotype of E(spl)mγ-HLH^UAS.cLb, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Cdk9^{D199N.Scer\UAS.SR} fully suppresses the increased number of type II neuroblasts in third instar larval brains resulting from either the expression of N^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} alone, E(spl)mγ-HLH^Scer\UAS.cLb alone or the co-expression of lilli^{Scer\UAS.SR.T:Zzzz\FLAG} and Su(Tpl)^{Scer\UAS.SR.T:Hsap\MYC}, under the control of Scer\GAL4^insc-Mz1407. The combined expression of Cdk9^{D199N.Scer\UAS.SR} and E(spl)mγ-HLH^Scer\UAS.cLb partially suppresses the increased number of neuroblasts in third instar larval brains resulting from the expression of N^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^insc-Mz1407.

The co-expression of Cdk9^{D199N.Scer\UAS.SR} also fully suppresses the increased number of neuroblasts in third instar larval brain lobes resulting from the expression of Su(H)^{Scer\UAS.T:Hsim\VP16} under the control of either Scer\GAL4^pnt-14-94 or Scer\GAL4^insc-Mz1407.

(Liu et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Cdk9^{D199N.Scer\UAS.SR}

Cdk9^D199N.UAS.SR

Name Synonyms

Secondary FlyBase IDs

References (1)

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