FB2024_03 , released June 25, 2024
Allele: Dmel\kugΔICD.3xGFP
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General Information
Symbol
Dmel\kugΔICD.3xGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0326665
Feature type
allele
Associated gene
Dmel\kug
Associated Insertion(s)
TI{TI}kugΔICD.3xGFP
Carried in Construct
Also Known As
fat2ΔICD-3xGFP
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Barlan et al., 2017)
gene targeting by homologous recombination
(Barlan et al., 2017)
Progenitor genotype
Associated Insertion(s)
TI{TI}kugΔICD.3xGFP
Cytology
Description

The intracellular domain of the endogenous kug gene (sequence corresponding to nucleotides 17382-19182) has been replaced with three copies of the GFP tag (this corresponds to the tag being inserted following amino acid residue 4321). A single loxP site is also present.

(Barlan et al., 2017)
Allele components
Component
Use(s)
Tagged with
GFP
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3L:20,022,682..20,024,482 [+]
Comment:

The intracellular domain of the endogenous kug gene (sequence corresponding to nucleotides 17382-19182) has been replaced with three copies of the Avic\GFP tag.

(Barlan et al., 2017)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      kugΔICD.3xGFP mutant cells in mosaic follicular epithelia exhibit induction of leading edge protrusions cell autonomously, as well as non-cell-autonomously in the wild type cells directly behind kugΔICD.3xGFP cells. Protrusion formation only occurs at the leading edges of these cells, not in the lateral cell edges. kugΔICD.3xGFP mutant cells also exhibit significantly elongated basal surfaces, indicative of a defect in trailing edge retraction.

      (Barlan et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      kugΔICD.3xGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (3)