UASt regulatory sequences drive expression of a mutated form of Spt5 in which amino acid residues 779-784 have all been mutated to Ala. The protein is tagged at the C-terminal end with two tandem copies of Tag:FLAG. Synonymous mutations have been introduced into the sequence so that it is resistant to the Spt5HMS00153 RNAi line.
Scer\GAL4Act5C.PI/Spt5779-784A.UAS.Tag:FLAG fails to rescue Spt5HMS00153
The co-expression of Spt5779-784A.Scer\UAS.T:Zzzz\FLAG fails to rescue the lethality induced by the expression of Spt5HMS00153 under the control of Scer\GAL4Act5C.PI.