FB2024_03 , released June 25, 2024
Allele: Dmel\LpinΔPAP.UAS
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General Information
Symbol
Dmel\LpinΔPAP.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0324127
Feature type
allele
Associated gene
Dmel\Lpin
Associated Insertion(s)
Carried in Construct
P{UAS-Lpin.ΔPAP}
Key Links
Transgenic product class
missense_variant
(Schmitt et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Schmitt et al., 2015)
Mutagen
in vitro construct
(Schmitt et al., 2015)
Progenitor genotype
Carried in construct
P{UAS-Lpin.ΔPAP}
Cytology
Description

UASt regulatory sequences drive expression of Lpin with a mutation in the catalytic motif previously shown to result in complete loss of phosphatidate phosphatase activity (cDNA GH19076 with D812E amino acid exchange).

(Schmitt et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Lpin
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of LpinΔPAP.Scer\UAS fails to suppress the larval growth defects, fat body cell size defects, and inability to pupariate seen in larvae expressing LpinGD14004 and raptorHMS02306 under the control of Scer\GAL4Cg.PA.

      (Schmitt et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      LpinΔPAP.UAS/Scer\GAL4r4 fails to rescue Df(2R)Exel7095/Lpine00680

      (Schmitt et al., 2015)
      Comments

      Expression of LpinΔPAP.Scer\UAS under the control of Scer\GAL4r4 fails to rescue the defects in fat body cell morphology and lipid droplet formation seen in Lpine00680/Df(2R)Exel7095 larvae, and increases larval lethality seen in these flies.

      (Schmitt et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      LpinΔPAP.Scer\UAS
      LpinΔPAP.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)