FB2024_03 , released June 25, 2024
Allele: Dmel\dapmDeg.UASp.EGFP
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General Information
Symbol
Dmel\dapmDeg.UASp.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0323611
Feature type
allele
Associated gene
Dmel\dap
Associated Insertion(s)
Carried in Construct
P{UASp-EGFP-dap.mDeg}
Key Links
Transgenic product class
missense_variant
(Swanson et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Swanson et al., 2015)
Mutagen
in vitro construct
(Swanson et al., 2015)
Progenitor genotype
Carried in construct
P{UASp-EGFP-dap.mDeg}
Cytology
Description

UASp regulatory sequences drive expression of mutated dap in which all consensus PIP degron residues have been mutated. The protein is tagged at the N-terminal end with EGFP.

(Swanson et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
dap
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of dapmDeg.Scer\UAS.P\T.T:Avic\GFP-EGFP under the control of Scer\GAL4prd.PU does not interfere with the normal cell cycle program of embryonic epidermal cells Expression under the Scer\GAL4en.PU driver does not disrupt growth or proliferation of the imaginal wing disc cells either. Nevertheless, expression of dapmDeg.Scer\UAS.P\T.T:Avic\GFP-EGFP driven by Scer\GAL4αTub84B.PL is lethal with about 90% of the embryos even failing to hatch but has no effect on the proliferation or differentiation of central and peripheral nervous system. However, the embryos display reduced S-phase index in the midgut while the cell number is unchanged, suggesting a defect in the endocycle. The endocycle progression is also disturbed in the ovarian follicle cells expressing dapmDeg.Scer\UAS.P\T.T:Avic\GFP-EGFP using the Scer\GAL4c323 driver or in the third instar larval salivary gland cells using the Scer\GAL4ptc-559.1 driver (manifested by reduced number of cells in S-phase as well as decreased nuclear size).

      (Swanson et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      dapmDeg.Scer\UAS.P\T.T:Avic\GFP-EGFP
      dapmDeg.UASp.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)