UAS regulatory sequences drive expression of LIM domains 2, 3 and 4 of Zasp52, tagged at the N-terminal end with a Tag:FLAG-Tag:StrepII-Tag:polyHis tag cassette.
Expression of Zasp52LIM234.Scer\UAS.FSH under the control of Scer\GAL4Mef2.PU, but not under the control of Scer\GAL4Hk-UH3, results in indirect flight muscle defects, as compared to controls.
Zasp52LIM234.UAS.FSH/Scer\GAL4Hk-UH3 fails to rescue Zasp52MI02988/Df(2R)BSC427
Expression of Zasp52LIM234.Scer\UAS.FSH under the control of Scer\GAL4Hk-UH3 fails to rescue the indirect flight muscles seen in Zasp52MI02988/Df(2R)BSC427 mutants.