FB2024_03 , released June 25, 2024
Allele: Dmel\dncRNAi.UAS.WIZ
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General Information
Symbol
Dmel\dncRNAi.UAS.WIZ
Species
D. melanogaster
Name
FlyBase ID
FBal0322917
Feature type
allele
Associated gene
Dmel\dnc
Associated Insertion(s)
Carried in Construct
P{UAS-dnc.RNAi.WIZ}
Also Known As
UAS-DncRNAi
Key Links
Transgenic product class
RNAi_reagent
(Scheunemann et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Scheunemann et al., 2012)
Mutagen
in vitro construct
(Scheunemann et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-dnc.RNAi.WIZ}
Cytology
Description

UAS regulatory sequences drive expression of an inverted repeat of a 580bp fragment of dnc transcript D (nucleotides 1617-2197) which targets five exons (exons 10-14 of transcript D) that are common to all dnc isoforms.

(Scheunemann et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
dnc
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Adult flies expressing dncdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4elav.PU show significant decrease in the amnestic cooling resistant memory component at 3 hr post-training in an olfactory memory assay compared to controls.

      (Scholz-Kornehl and Schwärzel, 2016)

      Expression of dncdsRNA.UAS.WIZ under the control of Scer\GAL4c739 in adults significantly reduces the anaesthesia-resistant component of short term (5 min) memory, compared to controls; expression under the control of Scer\GAL4NP0021 has no effect on the anaesthesia-resistant component of medium term (3 h) aversive olfactory memory, compared to controls; expression under the control of Scer\GAL44-59 significantly reduces long term (24 h after massed training) aversive olfactory memory, compared to controls.

      (Bouzaiane et al., 2015)

      Expression of dncdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4elav.PU results in defective short term memory, 3 min after olfactory conditioning, and defective anaethesia-resistant memory, but not defective anaesthesia-sensitive memory, when cold-amnestic treatment is applied 30 min before testing 3 h odor memory, as compared to controls.

      Expression of dncdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4GH298 or Scer\GAL4NP1227, but not Scer\GAL4Orco.PU, Scer\GAL4Mef2.247 or Scer\GAL4NP0225, significantly decreases short term memory performance 3 min after olfactory conditioning.

      Expression of dncdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4GH298, but not Scer\GAL4Mef2.247, results in defective anaethesia-resistant memory, but not defective anaesthesia-sensitive memory, when cold-amnestic treatment is applied 30 min before testing 3 h odor memory, as compared to controls.

      (Scheunemann et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhancer of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The reduced 3 hr olfactory memory characteristic for Dop2RΔ1 homozygous adults is not decreased further, and neither are any of its components (amnesia resistant or sensitive), by expression of dncdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4elav.PU in the mutant background.

      (Scholz-Kornehl and Schwärzel, 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      dncRNAi.UAS.WIZ
      dncRNAi
      (Scholz-Kornehl and Schwärzel, 2016)
      dncdsRNA.Scer\UAS.WIZ
      dncdsRNA.UAS.WIZ
      Name Synonyms
      Secondary FlyBase IDs
        References (6)