UAS regulatory sequences drive expression of a mutated form of FIG4 that carries the amino acid replacement C450S. The protein is tagged at the N-terminal end with Tag:MYC.
Scer\GAL4Mef2.PU/FIG4C450S.UAS.Tag:MYC rescues FIG4Δ1
Expression of FIG4C450S.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Mef2.PU fully rescues the lysosome expansion phenotype in somatic muscles of FIG4Δ1 homozygous third instar larvae.