A Drosophila codon-optimized Cas9 endonuclease fused to the Tag:NLS(Ude) nuclear localization signal is expressed under the control of UAS regulatory sequences (ORF cloned into P{10XUAS-IVS-Syn21-GFP-p10}, FBtp0073107).
abnormal developmental rate, with Scer\GAL4elav-C155, StimsgRNA.dual.snRNA:U6:96Ab
abnormal developmental rate, with Scer\GAL4nSyb.PS, StimsgRNA.dual.snRNA:U6:96Ab
abnormal developmental rate, with Scer\GAL4ple.PF, StimsgRNA.dual.snRNA:U6:96Ab
abnormal locomotor behavior | adult stage, with Scer\GAL4elav-C155, nAChRβ1U6.pCFD4
abnormal wound healing | adult stage, with RubiconTKO.GS04756, Scer\GAL4repo
decreased cell number | larval stage, with Scer\GAL4GMR31H09, ShmtTKO.GS04720
lethal | male, with Scer\GAL4elav-C155, StimsgRNA.dual.snRNA:U6:96Ab
partially lethal, with Scer\GAL4ple.TH-A, StimsgRNA.dual.snRNA:U6:96Ab
partially lethal - majority die, with Scer\GAL4elav-C155, StimsgRNA.dual.snRNA:U6:96Ab
partially lethal - majority die, with Scer\GAL4nSyb.PS, StimsgRNA.dual.snRNA:U6:96Ab
partially lethal - majority die, with Scer\GAL4ple.PF, StimsgRNA.dual.snRNA:U6:96Ab
adult marginal nerve wrapping glial cell, with RubiconTKO.GS04756, Scer\GAL4repo
embryonic/larval optic lobe | larval stage, with Scer\GAL4GMR31H09, ShmtTKO.GS04720
lamina | absent | larval stage, with Scer\GAL4GMR31H09, ShmtTKO.GS04720
marginal nerve, with RubiconTKO.GS04756, Scer\GAL4repo
medulla | larval stage, with Scer\GAL4GMR31H09, ShmtTKO.GS04720
optic lobe neuroepithelial cell | decreased number | larval stage, with Scer\GAL4GMR31H09, ShmtTKO.GS04720
Scer\GAL4elav-C155, StimUAS.cAa, Spyo\Cas9UAS.Tag:NLS(Ude) partially rescues StimsgRNA.dual.snRNA:U6:96Ab
Generated by Fillip Port and Simon Bullock, Medical Research Council, Cambridge.
Generated by Fillip Port and Simon Bullock, Medical Research Council, Cambridge.